AST - micro ii

Question 1

Lowest drug concentration that inhibits visible growth of organism

Accepted Answer

MIC

Question 2

Which of the following drugs should serum antimicbroal levels be monitored?

Accepted Answer

chloramphenicol, aminoglycosides, vancomycin

Question 3

T/F the Proteus swarm is accounted for when determing disk diffusion results

Accepted Answer

FALSE

Question 4

Extended spectrum beta lactasmase uses cefotaxime and ceftazimbe, it works with

Accepted Answer

beta-lactasmase in staphylocuus, beta-lactasmase in H. influenza, beta-lactasmase in E. coli

Question 5

T/F there is a linearish relationship bewteen broth dilutions and disk difsion with the succes of treatment

Accepted Answer

TRUE

Question 6

putting animicrobial agents in semi-liquid agar where you can innoculate mutliple patients on the same plate describes

Accepted Answer

agar dilution

Question 7

lowerst concentration of drug that kills 99.9% of bacteria tests

Accepted Answer

MBC

Question 8

T/F amingoglycoside screen tests the syngery of ampicillin an gentamcin

Accepted Answer

TRUE

Question 9

What are the disadvantages of microdilution?

Accepted Answer

less choice of drugs, skipped wells, expensive

Question 10

what is true about the D test

Accepted Answer

shows induced clindamycin resistant

Question 11

Microscan automation system

Accepted Answer

takes 16-48 hours

Question 12

T/F aminoglycodye screen is the test for MRSA

Accepted Answer

FALSE

Question 13

T/F patient results can be released if there is an out of range QC

Accepted Answer

FALSE

Question 14

What should be done to monitor patients reviecing antimicrobials with a narrow toxic therapeutic range

Accepted Answer

biological assay for serum antimicobials, chromatogrpahic assay for serum antimicobials, immunoassay for serum antimicobials

Question 15

What is true about modified carbapenem inactivation methods?

Accepted Answer

if susceptibily e. coli grows w/disk organism inactives carabpenem, suspect organism in water with meropenem; then disk onto lawn of e. coli

Question 16

How long does kirby-bauer have to be incubated?

Accepted Answer

18-24 hours

Question 17

infection due to bacteria may be treated with dose of antimicrobal agent for thay infection

Accepted Answer

susceptible

Question 18

postive ESBL tests indicates resistance for

Accepted Answer

cephalosporin, penicillin, aztreoname

Question 19

This provides guidance for empiric treatment and guidance of resistance patterns seen in a particular hosptial

Accepted Answer

antibiogram

Question 20

What do colonies inside the zone of inhibit of a disk diffusion indicated?

Accepted Answer

plated with mixed colonies, hetero-resistant strain present

Question 21

staphlococcus, s. pneumonia, beta strep that are susceptible to clinfamycin and resistant to erythocyin must undergo

Accepted Answer

d test

Question 22

What is false about standardization for antimirobial testing

Accepted Answer

normally use blod agar

Question 23

automated ASTmicrodilution with a flurormeter measures

Accepted Answer

hydrolysis of a flurogenic substrate

Question 24

What is true about Kibry-Bauer disks?

Accepted Answer

the concentration of the antiboitic on the disk is known, use dissetants with storage to avoid mositure build up

Question 25

What is the difference between microbroth and macrobroth dilution methods?

Accepted Answer

microbroth can look at multiple antimicrobials at once

Question 26

What is true about quality control?

Accepted Answer

Qc samples done the same way a patient sample should be, use ATCC strains, performed weekly if IQCP developed

Question 27

What is true about the Etest

Accepted Answer

MIC is POINT where growth stops, is a gradient diffusion along the plated strip

Question 28

Chromogen cephalosporin is used to detect

Accepted Answer

beta-lactasmase in staphylocuus, beta-lactasmase in H. influenza

Question 29

What is the result of having agar depth of 2mm?

Accepted Answer

larger zone of inhibtion

Question 30

bacteria testes falls into range in which the level of anticibriol needed cannot be achiebed with normal dose

Accepted Answer

intermediate

Question 31

This method evaulates the effectivness of antimicrobial in a patient

Accepted Answer

schilchter serum test

Question 32

What is true about the serum bactericidal test?

Accepted Answer

if trough titer > 1:32 and peak is > 1:64, antimicrobial is effective, have to keep paitent organism isolate on file, collect specimen at trough stage and peak stage

Question 33

T/F any drug can be cleared to be used to treat any type of infection at any type of site

Accepted Answer

FALSE

Question 34

The Vitek automation system

Accepted Answer

takes 8-24 hours, cannot be read manually, generate MIC based on an algorthirm (not a true MIC)

Question 35

automated AST on microbdilution with a photometer measures

Accepted Answer

turbidity

Question 36

What is true about ESBL?

Accepted Answer

tests to see if the bacteria has a beta-lactamase enzyme, uses disks with and without a beta-lactam inhibitor, (+) for enzyme >5mm increase of disk w/ inhibitor compared to disk w/o

Question 37

These infections shouls be monitored with serum bactericidial test

Accepted Answer

endocarditis, osteomyeltesis, bacterimia with GNB

Question 38

bacteria test not inhibited by drug concentration with normal doage

Accepted Answer

resistant

Question 39

Which of the following methods detect MIC?

Accepted Answer

Etest, microtube broth dilution, agar dilution

Question 40

Oxacillin agar screen, PBP2a, chromagar, and PCR can be used to detect

Accepted Answer

MRSA

Question 41

T/F aminoglycodye screen is the test for VRE

Accepted Answer

TRUE

Question 42

What is true about carbanp?

Accepted Answer

hydrolysis method cause a color change

Question 43

T/F oxacillin disk is used to screen for penicillin resistant Strep pneumoniase

Accepted Answer

TRUE

Question 44

Which method not standardized for anaerobes?

Accepted Answer

Kirby-Bauer

Question 45

Which method is goo for fastidios orgnaims that don’t grow well in broth systems

Accepted Answer

Etest

Question 46

What is true about the Kirby-Bauer method?

Accepted Answer

can test up to 12 drugs per plate, Have inoculate the MH plate within 15 min of making suspesion, have to add the disks with 15 min of innoculating plate

Question 47

T/F Disk diffions tests are usually read through the back of the plates except for blood agar

Accepted Answer

TRUE

Question 48

T/F MHA with blood is used for S. pneumonia and Streps with kibry-bauer

Accepted Answer

TRUE

AST - micro ii

Questions 48

AST - micro ii

Questions 48