Why is amp necessary on second and third plate?

to confirm that the ampicillan is working

What does Bt produce?

a nasty protein that kills insects

Which plate in the transformation will glow? Why?

LB/amp/ara- cuz the ara makes it glow

Coomasie Blue is used to

stain protein gels and see bands

What is the point of making a standard curve?

to determine unknown concentrations

breaks down carbohydrates into sugars

All of these things are true of PCR EXCEPT

restriction enzymes must be added to break apart the strands

A monoclonal antibody is (check all that apply)

an antibody with the same variable region and many copies are made in lab, a protein

Why is buffer made when working with a protein and a spec 20?

to ensure the protein does not denature, to calibrate spec

True or False- PCR can make a few copies of a person's entire DNA sequence.

False- it copies a short section of a persons DNA many times

What does this graph have to do with biological molecules?

its tells you the lambda max of a molecule

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SPRING BIOTECH FINAL

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Questions 35

Answers

1
What is the graph below called?
absorption spectrum
standard solutions
bell curve
standard curve
2
Why is amp necessary on second and third plate?
to confirm plasmid was put into bacteria
to help bacteria grow
to ensure that bacteria will glow
to confirm that the ampicillan is working
3
What samples are used to make a standard curve?
solutions
known dilutions
unknown dilutions
dilutions
4
What does Bt produce?
a nasty protein that kills weeds
a nasty protein that kills insects
a nasty carbohydrate that kills plants
a nasty carbohydrate that kills insects
5
Which plate in the transformation will glow? Why?
LB/amp- cuz the amp on the plate
LB, cuz all things grow on it
LB/amp/ara- cuz the ara makes it glow
LB/amp/ara- cuz the amp makes it glow
6
PAGE separates _______ by _____ and _____.
protein, size, shape
DNA, size, shape
DNA, size, charge
protein, size, charge
7
The 3 steps of PCR in order are
denature, anneal,extend
copy, denature, extend
duplicate, extend, denature
extend, anneal, denature
8
Bees pollinating a flower is an example of
parasitism
mutualism
commensalism
predation
9
The enzyme responsible for PCR is
Bt
amylase
Ligase
Taq Polymerase
10
Coomasie Blue is used to
unwind proteins in solution
stain protein gels and see bands
give color to protein dilutions
stain DNA gels and see bands
11
What is the point of making a standard curve?
to determine known concentrations
to determine unknown concentrations
to use the spec alot
to make a lot of dilutions
12
If the DNA reads AGTCAGTC, the RNA will read
UCAGUCAG
TATGACA
TCAAGTCAG
TCAGACTAG
13
Amylase
breaks down lipids into fats
breaks down sugar into carbohydrates
breaks down carbohydrates into sugars
breaks down protein into amino acids
14
What enzyme (s) would they use to cut out the "blue" box of DNA? What is the the size?
EcoR1 and BAM, 200
EcoR1, 300
BAM, 200
EcoR1 and BAM, 250
15
An example of a GMO is
a bacteria that produces a nasty protein
A person with 2 different colored eyes
A tree that makes sweet apples
A banana with edible vaccine
16
The gene we amplified that tells us we have plant DNA is
Rubisco
35S
Bt
alu
17
The step of PCR that copies DNA is
anneal
denature
heat
extension
18
What is the lambda MIN for red?
400 nm
440 nm
620 nm
520nm
19
Parthenogenesis is
what wolbachia does to change an insect
how insects mate
a form of sexual reproduction
a form of asexual reproduction
20
All of these things are true of PCR EXCEPT
nucleotides must be added to make more DNA
it works by heating/cooling the DNA
restriction enzymes must be added to break apart the strands
primers must be added to attach to the sites to be copied
21
If you have a tiny amount of DNA and need to make more, you use this process to do it
digestion with restriction enzymes
polymerase chain reaction
centrifuging
electrophoresis
22
DNA should be loaded into the ________ end because DNA's charge is __________
negative, positive
negative, negative
positive, negative
positive, positive
23
How many copies of DNA are generated from 30 cycles of PCR?
thousands
millions
trillions
billions
24
A monoclonal antibody is (check all that apply)
an antibody with the same variable region and many copies are made in lab
a protein
an antibody with different variable regions and many copies are made in lab
made by the body
25
Wolbachia is a (n)
bacteria
insect
virus
human
26
The absorbance is .66 and the slope is y= .225x, what is the concentration of the unknown?
2.93 mg/ml
2.93 au
.1485 mg/ml
.34 mg/ml
27
Why is buffer made when working with a protein and a spec 20?
to make sure the enzyme keeps its shape and good pH
ensure the protein keeps its shape, to make sure spec works
to see colors of light more clearly in the spec
to ensure the protein does not denature, to calibrate spec
28
The part of the insect we tried to extract DNA from was the _______
thorax
head
abdomen
legs
29
Light absorbed means
it passes through a sample
it cannot be seen (visible)
it can be seen
it reflects light
30
The following are examples of amino acids
hemoglobin, amylase
lysine, glutamine
polymerase, cofactor
tris, monophosphate
31
True or False- PCR can make a few copies of a person's entire DNA sequence.
True- it copies a short section of a persons DNA many times
False- it copies a short section of a persons DNA many times
True- it copies a persons entire sequence
False- it copies some of a persons sequence
32
What does this graph have to do with biological molecules?
it tells you what colors of light are absorbed
it tells you the concentration of unknowns
it tells you how different wavelengths
its tells you the lambda max of a molecule
33
What is used in an ELISA to give a color change?
substrate
secondary antibody
primary antibody
enzyme
34
Symbiosis is the living of ___________ organisms in ________ place.
different, different
similar, different
different, the same
similar, the same
35
The central dogma biology states that information flows from
DNA to protein to RNA
RNA to protein to DNA
DNA is soooo cool
DNA to RNA to protein

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