stud
bud
Sign up
Log in
Home
Library
Explore
SAT
More
MI PCR Flashcards | StudBud
Explore
/
MI PCR
MI PCR
11 terms
·
StudBud Administration
·
501 plays
Imported from external platform
Flashcards
11 cards
F
Practice quiz
P
Host live
L
Questions
11
Answers
1
What color are a lady bugs spots?
black
orange
red
white
2
What allows the primer to anneal?
Lowering the temperature for 20-40 seconds
Denaturase
DNA polymerase
Increasing the temperature for 20-40 seconds
3
What is the purpose of Denaturation phase?
To heat the DNA and other products
To activate the DNA polymerase
To break the hydrogen bonds between the DNA strands and separate them.
To allow 20-30 seconds for the DNA to be copied
4
Which of these is NOT true about initialization?
It runs every 2-10 minutes
The purpose is to activate polymerase and denature contaminates
The components are heated to 94 degrees C
It is done once for 2-10 minutes
5
PCR
(select all that apply)
is sometimes called a molecular photocopier
allows for amplification of DNA sequence for a particular gene
is time consuming and expensive
Is used for detection of genetic diseases and more
6
Enzymes, like those in the human body, have ideal temperatures. What is an ideal temperature for taq polymerase?
74
64
84
94
7
After the primer establishes a starting point, what enzyme helps to build the DNA strand?
DNA elongase
DNA polymerase
DNA ligase
DNA primerase
8
How many times do the cycles repeat?
50-100
10-20
20-40
30-50
9
What enzyme is involved in the process?
DNA polymerase
Restriction enzymes
RNA polymerase
DNA ligase
10
If you start with one strand of DNA, how many copies will you have after 3 cycles?
8
16
4
2
11
What is the height of a basketball hoop in feet?
12
6
8
10
