What happens during extension?

DNA polymerase extends the primers using free nucleotides

What is required to perform a PCR?

Template DNA, DNA polymerase, free nucleotides, primers

Where do primers bind?

Flanking the target region on opposite DNA strands

What happens during denaturation?

Breaks hydrogen bonds and makes single strands

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Polymerase Chain Reaction (PCR)

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Basics of PCR to year 12 biology students

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Questions 16

Answers

1
What is the process called when primers bond to a section of DNA?
Extension
Addition
Annealing
Tempering
2
What happens during extension?
Double stranded section extended with free bases
DNA polymerase extends the primers using free nucleotides
Double stranded section extended using DNA helicase
Double stranded section is extended with free nucleotides
3
In PCR, from which species is DNA polymerase isolated from?
Homo sapiens
Lactobacillus acidophilus
Plasmodium falciparum
Thermus aquaticus
4
What is the optimum temperature for DNA polymerase?
62°C
71°C
72°C
61°C
5
Which of the following is not a use for PCR?
Infectious disease detection and identification
Treating diseases
Genetic testing for heritable conditions
Forensic DNA profiling
6
What does PCR stand for?
polymer chain reaction
polymerase cascade reaction
polypeptide chain reaction
polymerase chain reaction
7
What quality of Thermus aquaticus makes it favourable for PCR?
Thermophobic
Hydrophilic
Thermophilic
Neutrophilic
8
What is required to perform a PCR?
Template DNA, RNA polymerase, free nucleotides, primers
Template DNA, helicase, free nucleotides, primers
Template DNA, DNA polymerase, DNA helicase, free nucleotides
Template DNA, DNA polymerase, free nucleotides, primers
9
At what temperature does annealing occur?
73°C
45°C
95°C
55°C
10
Where do primers bind?
Flanking the target region on the same DNA strand
Only upstream the target region
Flanking the target region on opposite DNA strands
Only downstream the target region
11
What is PCR?
natural DNA replication
artificial DNA replication
artificial DNA synthesis
identical to DNA replication
12
What do primers do?
Extracts sections of DNA
Bind to a section of DNA
Catalyse the reaction
Copy sections of DNA
13
What happens during denaturation?
Breaks ionic bonds and makes a single strand
Joins hydrogen bonds and makes a single strand
Breaks hydrogen bonds and makes single strands
Denatures DNA into free nucleotides
14
At what temperature does DNA denature?
100°C
90°C
85°C
95°C
15
What is used in PCR to separate and bind DNA strands?
A cycle of heating and condensing
DNA helicase enzyme
A cycle of boiling and evaporating
A cycle of heating and cooling
16
After each cycle of PCR, the amount of DNA is increased...
Expentially
By 2
Exponentially
By squaring the amount before

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