Where do primers bind?

Flanking the target region on opposite DNA strands

What is required to perform a PCR?

Template DNA, DNA polymerase, free nucleotides, primers

What happens during denaturation?

Breaks hydrogen bonds and makes single strands

What happens during extension?

DNA polymerase extends the primers using free nucleotides

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Polymerase Chain Reaction (PCR)

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Basics of PCR to year 12 biology students

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Questions 16

Answers

1
What is PCR?
natural DNA replication
artificial DNA replication
identical to DNA replication
artificial DNA synthesis
2
At what temperature does DNA denature?
85°C
90°C
100°C
95°C
3
Where do primers bind?
Only downstream the target region
Flanking the target region on the same DNA strand
Only upstream the target region
Flanking the target region on opposite DNA strands
4
At what temperature does annealing occur?
95°C
45°C
55°C
73°C
5
What is the process called when primers bond to a section of DNA?
Addition
Tempering
Annealing
Extension
6
After each cycle of PCR, the amount of DNA is increased...
Expentially
By 2
By squaring the amount before
Exponentially
7
What is required to perform a PCR?
Template DNA, RNA polymerase, free nucleotides, primers
Template DNA, DNA polymerase, DNA helicase, free nucleotides
Template DNA, helicase, free nucleotides, primers
Template DNA, DNA polymerase, free nucleotides, primers
8
What happens during denaturation?
Breaks ionic bonds and makes a single strand
Denatures DNA into free nucleotides
Breaks hydrogen bonds and makes single strands
Joins hydrogen bonds and makes a single strand
9
In PCR, from which species is DNA polymerase isolated from?
Plasmodium falciparum
Thermus aquaticus
Lactobacillus acidophilus
Homo sapiens
10
Which of the following is not a use for PCR?
Infectious disease detection and identification
Treating diseases
Genetic testing for heritable conditions
Forensic DNA profiling
11
What quality of Thermus aquaticus makes it favourable for PCR?
Hydrophilic
Neutrophilic
Thermophilic
Thermophobic
12
What does PCR stand for?
polymerase chain reaction
polymerase cascade reaction
polymer chain reaction
polypeptide chain reaction
13
What happens during extension?
Double stranded section extended with free bases
Double stranded section is extended with free nucleotides
Double stranded section extended using DNA helicase
DNA polymerase extends the primers using free nucleotides
14
What is used in PCR to separate and bind DNA strands?
A cycle of heating and cooling
A cycle of boiling and evaporating
A cycle of heating and condensing
DNA helicase enzyme
15
What is the optimum temperature for Taq polymerase?
72°C
71°C
61°C
62°C
16
What do primers do?
Copy sections of DNA
Bind to a section of DNA
Extracts sections of DNA
Catalyse the reaction

Polymerase Chain Reaction (PCR) Flashcards | StudBud