When measuring a specific amount of a substance, which pipette would you use?

The smallest that includes the amount needed.

Gram staining will stain purple if

bacteria have a thick cell wall made of peptidoglycan

All of the following symbols in some way represent:

The substance is a health hazard.

What is missing from the following graph?

All of the choices are missing and should be on the graph.

A biosafety cabinent is on the left.

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BACE Tools, Equipment and Symbols Review

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Questions 100

Answers

1
Name the pictured piece of lab equipment.
Test tube holder
Graduated Cylinder
Foreceps
Crucible tongs
2
In the following picture, which is the most concentrated test tube in the protein assay?
Tube 5
Tube 1
Tube 3
Tube 2
3
Is the substance flammable according to the SDS sheet?
No
Yes
4
Used to maintain records of experiments
Lab Notebook
Agarose or acrylamide
PCR Machine
Centrifuge
5
What is the shape of the red bacteria?
bacillus (rod)
spirillus (spiral)
coccus (sphere)
6
When measuring a specific amount of a substance, which pipette would you use?
The smallest that includes the amount needed.
The one that goes to the highest amount.
Whichever one is the closest - even if you have to split the amount.
The smallest one available.
7
What does the symbol below represent?
Hot surface
Oxidizer
Toxic or poisonous materials
Eye wash station
8
Gram staining will stain purple if
bacteria have no cell wall
bacteria have a thin cell wall made of peptidoglycan
bacteria two thick cell wall made of peptidoglycan
bacteria have a thick cell wall made of peptidoglycan
9
What does the symbol below represent?
Do not enter
No eating or drinking
Harmful or irritant
Biohazard
10
Type of bacteria.
Cocci
Basophil
Yeast
Bacilli
11
These bacteria are
Gram-positive
Gram-negative
12
Name the pictured piece of lab equipment.
Spatula or scoopula
Foreceps
Test tube rack
Test tube holder
13
Used to mix small volume solutions
Balance
Vortex mixer
PCR machine
pH meter or litmus paper
14
What does this symbol represent?
Radiation
Corrosive
Wash your hands
Harmful or irritant
15
Which of the following amplifies DNA by heating and cooling?
Autoclave
Vortex mixer
PCR machine
Balance
16
Used to measure the concentration of hydrogen ions in a sample.
Electrophoresis
Autoclave
Spectrophotometer
pH meter
17
What does the symbol below represent?
Eye wash station
Safety Shower Location
Do not drink the water
Toxic or poisonous materials
18
What shapes are not bacteria cells?
Bacillus
Helixium
Spirillum
Coccus
19
The lab equipment pictured is
an electrophoresis chamber
a mini microcentrifuge
a vortex mixer
a PCR machine
20
These bacteria are
Gram-positive
Gram-negative
21
When weighing a powder chemical, can you put any excess you scooped out back into the bottle?
Yes.
It depends on the chemical.
Absolutely not!
As long as you didn't contaminate it.
22
The lab equipment pictured is
a micropipette
a microcentrifuge tube
an Erlenmeyer flask
a graduated cylinder
23
What does the symbol below represent?
Electrical
High voltage
Flammable
Radiation
24
What does this symbol represent?
Hospital
Needs assistance
Help
First Aid
25
When trying to determine the density of a substance, what values do you need to calculate it?
volume and density
mass and weight
volume and height
mass and volume
26
All of the following symbols in some way represent:
Something is on fire or will be soon.
The substance is an environmental hazard.
The substance is a health hazard.
This substance should never be used for anything.
27
What is the shape of the green bacteria?
coccus (sphere)
spirillus (spiral)
bacillus (rod)
28
Highly sensitive tool used to measure mass using a chamber to prevent the samples from being affected by air currents.
PCR Machine
Spectrophotometer
Analytical Balance
Balance
29
When completing a serial dilution, which would be the LEAST concentrated sample?
It is impossible to tell without seeing it
The last tube in the series
The first tube in the series after the stock solution
The stock solution used to start the dillution
30
Gram staining is a way to identify bacteria
True
False
31
When placing plates streaked with E.coli, what is the proper way to place them in the incubator
Upside down (bottom up)
Top side up (lid side up)
32
What is the measurement shown on this graduated cylinder?
34mL
38mL
40mL
42mL
33
What is missing from the following graph?
The main title.
The x axis is not labeled
All of the choices are missing and should be on the graph.
Units are not given for the x axis
34
What does the symbol below represent?
Do not drink the water
Safety Shower Location
Toxic or poisonous materials
Eye wash station
35
Main ingredient of a DNA gel and a protein gel?
PCR machine
Centrifuge
acrylamide; agarose
agarose; acrylamide
36
The three shapes of bacteria are...
rod, square, chair
rod, sphere, spiral
rod, circle, chair
37
The lab equipment pictured is
agar
an incubator
a petri dish
agarose
38
When measuring the weight of a substance, which of the following tools would you not use in the process?
graduated cylinder
scale
weighing boat
scoop/spatula
39
Name the pictured lab tool.
Petri dish tape
Masking tape
Autoclave tape
Labeling tape
40
Which is correct?
A fume hood is on the left.
A biosafety cabinent is on the left.
A biosafety cabinet is on the right.
These both serve the same purpose.
41
Name the pictured piece of lab equipment.
Graduated cylinder
Erlenmeyer Flask
Carboy
Media Bottle
42
What is the appropriate temperature for bacteria growth for an incubator?
60° C
23° C
37° C
92° C
43
What does this symbol represent?
No eating
No Eating or Drinking
Food Allowed
44
What does the symbol below represent?
Eye wash station
Toxic or poisonous materials
Safety Shower Location
Do not drink the water
45
Used to measure absorbance of light (concentration of a sample)
Lab notebook
Centrifuge
SOP
Spectrophotometer
46
Identify the meaning of the attached symbol
Fire Extinguisher Location
Defibrillator or AED Location
Hot surface
Toxic Waste
47
What does the symbol below represent?
High UV rays
Eye wash station
Face shield required
Goggles required
48
Name the pictured piece of lab equipment.
Micropipette
Dropper pipette
Macro pipette
Transfer pipette
49
What is the purpose of Gram staining?
is used to classify bacteria
includes all of these
detects peptidoglycan in the cell walls of bacteria
makes bacteria visible under microscope
50
What is the pictured piece of lab equipment?
A balance or scale
A vortex mixer
A plate reader
A hotplate stirrer
51
Name the pictured piece of lab equipment.
Pipette pumps
Serological pipettes/pipettes
Transfer pipettes
Micropipettes
52
When streaking a plate, what would be a reason there would be no growth the following day?
The wrong growing media was used
Incubator was at the wrong temperature (too hot)
Bacteria colony was dead/not concentrated enough
All of the choices could be correct
53
which of the following is not used in the Gram staining
Counter stain
Crystal violet
All of these are used
Gram iodine
54
The lab equipment pictured is
a vortex mixer
an electrophoresis chamber
a pcr machine
a microcentrifuge tube
55
What is the shape of the blue bacteria?
Spirillus (spiral)
Bacillus (rod)
Coccus (sphere)
56
What does this symbol represent?
Biohazard
Harmful or irritant
Radiation hazard
Radioactive
57
Name the pictured piece of lab equipment.
Graduated Cylinder
Test tube holder
Foreceps
Crucible tongs
58
Which will change pH faster when an acid is added?
Water
Buffer
59
What should you do if you get NaHCO₃ in your eyes?
Information not available.
Go out into the fresh air or apply oxygen to the eyes
Wash eyes out with soap and water
Remove contacts, flush eyes with water for 15 min.
60
In gel electrophoresis, DNA's charge is _________ and it runs toward the ________ side.
negative; postivie
neutral/negative
positive, negative
negative/neutral
61
Name the pictured piece of lab equipment.
Graduated cylinders
Erlenmeyer Flasks
Media Bottles
Carboys
62
Identify the meaning of the attached symbol
None of the choices are correct
Protective Clothing Required
Eye Protection Required
Biohazardous Waste
63
When completing a serial dillution what would the concentration be in the final tube?
1x105
1x104
1x10
1x101
64
Used to measure mass
pH meter or litmus paper
SOP
spectrophotometer
Balance
65
Name the pictured piece of lab equipment.
Transfer pipettes
Dropper pipettes
Serological pipettes/pipettes
Micropipettes
66
Name the pictured piece of lab equipment.
Centrifuge
Graduated Cylinder
Crucible
Macro pipette
67
What does the symbol below represent?
Hot surface
First Aid Station
Oxidizer
Toxic or poisonous materials
68
What does this symbol mean?
Goggles required
Eye wash station
Eye protection
Splash hazard
69
During gram staining, what do you use after Iodine?
Iodine
Alcohol
Safrarin
Crystal Violet
70
What is the 3rd step in the gram staining process?
Rinse with water
Decolorize with alcohol
Add iodine
Add safranin
71
Name the pictured piece of lab equipment.
Thermocycler
Microcentrifuge
Ice machine
PCR Machine
72
What is the name of the chemical this (M)SDS sheet is for?
Sodium Chloride
Sodium Hydroxide
Sodium Bicarbonate
It cannot be determined from this data sheet.
73
Separates substances based on density
Agarose or acrylamide
PCR Machine
Centrifuge
Autoclave
74
What is the temperature reading on the thermometer below?
90°
85°
65°
95°
75
Used to sterilize solutions, trash, supplies often with steam, heat & pressure
Vortex mixer
Autoclave
PCR machine
SOP
76
What does the symbol below represent?
Flammable material
Fire blanket
High voltage
Corrosive
77
The lab equipment pictured is
a beaker
an Erlenmeyer flask
a micropipette
a graduated cylinder
78
What does the symbol below represent?
Harmful or irritant
Gloves required
Hot surface
Goggles required
79
Name the pictured piece of lab equipment.
Standard incubator
Shaking incubator
Plate reader
Autoclave
80
Name the pictured piece of lab equipment.
Mortor and pestle
Evaporating dish
Carboy
Crucible
81
Which is Gram Negative
Left: Gram Negative
Right: Gram Negative
82
What biomolecule does the Bradford assay test for?
Antibodies
Sugar
DNA
Protein
83
How can you determine if something was heated to the proper temperature during sterilization?
Make sure it was in the autoclave for the required time.
Check the autoclave tape for temperature change.
Feel it with your hand to see if it is still hot hours after.
Take the temperature of the substance after autoclaving.
84
What does this symbol represent?
Corrosive
Radiation hazard
Flammable material
Biohazard
85
Identify the meaning of the attached symbol
Fire Extinguisher Location
Emergency Exit
Toxic Waste
Eye Protection Required
86
Used to see if a liquid is acidic or a basic
Balance
pH meter or litmus paper
Spectrophometer
SOP
87
When completing a serial dilution, which would be the MOST concentrated sample?
It is impossible to tell without seeing it
The stock solution used to start the dillution
The first tube in the series after the stock solution
The last tube in the series
88
Name the pictured piece of lab equipment.
Ice machine
Shaking water bath
Thermocycler
Microcentrifuge
89
Directions on how to perform a procedure can be found in using the a(n)
Spectrophotmeter
Lab notebook
pH meter or litmus paper
SOP
90
Type of bacteria
Yeast
Rods
Sperm
Cocci
91
The lab equipment pictured is
a macropipette
a micropipette
a microcentrifuge tube
a graduated cylinder
92
What would you refer to when disposing of a chemical in the lab?
The SDS
The internet (on a reputable site)
The bottle
All of the choices are correct
93
What is the chemical formula for the product listed?
NO2C3
Na2CO4
NaHCO3
It cannot be determined from this data sheet.
94
The lab equipment pictured is
a micropipette
a transfer pipette
a graduated cylinder
a microcentrifuge tube
95
Name the pictured piece of lab equipment.
Erlenmeyer Flask
Spatula or scoopula
Bunsen burner
Carboy
96
Name the pictured piece of lab equipment.
Shaking water bath
Ice machine
Thermocycler
Autoclave
97
The lab equipment pictured is
an Erlenmeyer flask
a micropipette
a graduated cylinder
a beaker
98
Name the pictured piece of lab equipment.
Mortor and pestle
Evaporating dish
Crucible
Carboy
99
What is the pictured piece of lab equipment?
A balance or scale
A hotplate
A hotplate stirrer
A vortex mixer
100
How would you determine if you need to wear gloves with a particular chemical?
Don't wear them and make sure to wash your hands afterwards.
Look it up on the SDS worksheet
Take a guess and hope you are right.
Look in your lab notebook.

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