What happens during extension?

DNA polymerase extends the primers using free nucleotides

What is required to perform a PCR?

Template DNA, DNA polymerase, free nucleotides, primers

What happens during denaturation?

Breaks hydrogen bonds and makes single strands

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IB DP Polymerase Chain Reaction (PCR)

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Basics of PCR to year 12 biology students

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Questions 14

Answers

1
At what temperature does DNA denature?
85°C
90°C
100°C
95°C
2
What is the process called when primers bond to a section of DNA?
Addition
Annealing
Tempering
Extension
3
What happens during extension?
Double stranded section is extended with free nucleotides
DNA polymerase extends the primers using free nucleotides
Double stranded section extended using DNA helicase
Double stranded section extended with free bases
4
What is used in PCR to separate and bind DNA strands?
DNA helicase enzyme
A cycle of heating and condensing
A cycle of boiling and evaporating
A cycle of heating and cooling
5
What is required to perform a PCR?
Template DNA, DNA polymerase, DNA helicase, free nucleotides
Template DNA, RNA polymerase, free nucleotides, primers
Template DNA, helicase, free nucleotides, primers
Template DNA, DNA polymerase, free nucleotides, primers
6
At what temperature does annealing occur?
95°C
45°C
55°C
73°C
7
What quality of Thermus aquaticus makes it favourable for PCR?
Thermophobic
Thermophilic
Neutrophilic
Hydrophilic
8
In PCR, from which species is DNA polymerase isolated from?
Lactobacillus acidophilus
Plasmodium falciparum
Homo sapiens
Thermus aquaticus
9
What is PCR?
identical to DNA replication
natural DNA replication
artificial DNA replication
artificial DNA synthesis
10
What do primers do?
Catalyse the reaction
Extracts sections of DNA
Copy sections of DNA
Bind to a section of DNA
11
After each cycle of PCR, the amount of DNA is increased...
Expentially
By 2
By squaring the amount before
Exponentially
12
Which of the following is not a use for PCR?
Forensic DNA profiling
Treating diseases
Infectious disease detection and identification
Genetic testing for heritable conditions
13
What happens during denaturation?
Joins hydrogen bonds and makes a single strand
Denatures DNA into free nucleotides
Breaks hydrogen bonds and makes single strands
Breaks ionic bonds and makes a single strand
14
What does PCR stand for?
polypeptide chain reaction
polymer chain reaction
polymerase cascade reaction
polymerase chain reaction

IB DP Polymerase Chain Reaction (PCR) Flashcards | StudBud