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Gene Cloning, PCR, CRISPR Flashcards | StudBud
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Gene Cloning, PCR, CRISPR
Gene Cloning, PCR, CRISPR
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Answers
1
What are the pro(s) of gmo's?
Avoidance of pests/disease
Medicine (insulin)
Efficient food production
All of the options
2
The components of CRISPR CAS 9 are:
The bacterial DNA and the CAS 9 enzyme
The guide RNA and the CAS 9 protein
The recombinant DNA
3
Taq
Polymerase is used in PCR because
Human Polymerase doesn't do extension
Human Polymerase is too slow
Human Polymerase would denature
Human Polymerase would work just fine
4
What are the principal problems to use CRISPR ?
All of the above
Bioterrorism
Ethics
Off-target effects
5
In PCR, the Denaturing stage happens at
320 C
520 C
>900 C
640 C
6
What is a benefit of GMO crops?
Drought resistant
Added nutrients
All of the above
Pest and disease resistant
7
What enzyme joins the two 'sticky-ends' during gene cloning?
DNA bindase
DNA polymerase
DNA joinase
DNA ligase
8
What protein does CRISPR utilize?
Oxytocin
Hemoglobin
Cas9
Actin
9
Gene cloning uses _________ and needs ________.
plasmids; restriction enzymes
respiration; water
fat; energy
water; carbondioxide
10
What does CRISPR provide for our society ?
The ability to edit genes of an organism
none of the above
The ability to enhance the human mind
The ability to allow humans to fly
11
What does CRISPR stand for ?
Clustered regularly interspaced short parental repeats
Cellar regularly infected short palindrome repeats
Clustered regularly interspaced short palindromic repeats
none of the following
12
The three steps of PCR, in order, are
Annealing, Denaturing, Extension
Denaturing, Annealing, Extension
Denaturing, Annealing, Extrapolating
Disassembling, Annealing, Extension
13
In PCR, the solution is heated to
Separate complementary DNA strands.
Denature the enzymes.
Separate the RNA primers from the DNA strands.
Anneal complementary DNA strands.
14
What does PCR stand for?
Polymerase chain reaction
Polymer channel reaction
Polymer chain reaction
Polymerase channel reaction
15
PCR is used to...
Amplify a DNA sample
Catch criminals by separating DNA strands
Create new genes
Break DNA up into single bases
16
CRISPR is unique in that
It is a hoax
It changes the genome
it can bring the dead back to life
it is capable of cutting DNA strands
17
Which is NOT part of the process of gene cloning technique?
insert antibiotics into the vector
insert the plasmid into a vector
insert the gene into a plasmid
isolate the gene of interest
18
Can CRISPR cut through DNA ?
Yes
no
19
A gene is...
A segment of DNA that codes for something
An Allele or trait
All the DNA coded in a nucleus
RNA
20
Where was CRISPR discovered in the wild?
In bacteria as a defence against viruses
on the skin of an apple
And a human respiratory system
All of the above
21
Gene cloning is
The process of figuring out what genes are on or off in cells
Making multiple identical copies of DNA
When you make bacteria glow in the dark
When you use genes to clone an organism
22
PCR means Polymerase Chain Reaction
True
False
23
What is CRISPR?
Edits genomes
Erases genomes
Garden Gnomes
Artificial Intelligence
24
A GMO product is indistinguishable from the original.
True
False
25
GMO means
Genetically Modified Organism
Great Monday Off
Great Mean Organization
Genetically Made Organ