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cell fractionation and ultracentrifugation Flashcards | StudBud

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cell fractionation and ultracentrifugation

7 terms·StudBud Administration·12 plays

A biology quiz to make sure lower sixth students understand the basics of cell fractionation.

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Questions 7

Answers

1
why is a cold solution required
so the pH stays the same
so you don't burn yourself
to reduce osmosis
to reduce enzyme activity
2
why is an isotonic solution required
to separate the organelles properly
so the pH stays the same
to the organelles from being broken down
so the water potential stays the same
3
what is the independent variable of differential centrifugation?
the speed at which the organelles are spun
the density of the organelles
the type of cell used
the temperature at which the organelles are spun
4
what is the fluid on top on the pellet called?
isotonic
supernatant
homogenate
filtrate
5
what is the purpose of cell fractionation?
because biologists have nothing better to do
to break down cells
to study specific organelles
to break down tissues
6
what does the buffer solution do?
stabilize the pH
prevent organelles from denaturing
prevent organelles from shrinking
prevent organelles from bursting
7
what is the second step of cell fractionation?
cutting of tissues
centrifugation
homogenization
filtration of the suspension from the homogenate