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Culture techniques/Abx sensitivity Flashcards | StudBud
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Culture techniques/Abx sensitivity
Culture techniques/Abx sensitivity
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Questions
30
Answers
1
No hemolysis on a BAP plate is called:
Beta
Delta
Alpha
Gamma
2
What fact is NOT considered to be a significant contributor to the development of bacterial resistance?
Normal concentrations of antibiotics for half the duration
Low concentrations of antibiotics for longer durations
Higher concentrations of antibiotics
Abx the do not reach the site of infection in concentrations exceeding MIC
3
Colony counts indicate infection presence.
False
True
4
Tube caps can be set down during inoculation.
True
False
5
What is the purpose of colony counts?
To determine CFUs
To measure temperature
To check for motility
To assess color
6
Which method tests bacterial motility?
Colony count
Gram stain
Hanging drop prep
Sensitivity testing
7
What does MIC stand for?
Maximum Infection Count
Maximum Inhibitory Concentration
Minimum Infection Count
Minimum Inhibitory Concentration
8
What does 'R' indicate in sensitivity testing?
Receptive
Rare
Responsive
Resistant
9
What is the significance of a 0.5 McFarland suspension?
Standardized turbidity
Color measurement
Temperature control
Volume assessment
10
Which method is NOT used to check for bacterial motility?
TSI
SIM
Hanging drop prep
Wet prep
11
What is the purpose of the hanging drop prep?
To cool samples
To check for movement
To stain bacteria
To count colonies
12
Routine cultures are incubated for _____ hours before initial examination.
48
18
32
72
13
What is a key characteristic of colony morphology?
Color and size
Temperature and pressure
Sound and smell
Shape and weight
14
The mechanism of action of beta-lactams is interference with
Protein sythesis
Cell wall synthesis
DNA synthesis
The uptake of nutrients
15
Procedure that involves application of undiluted urine directly on a test plate:
Direct sensitivity test
Indirect sensitivity test
Bauer-Kirby method
Disk susceptibility method
16
Where/how should agar plates be placed in the incubator?
Upside down
Right side up
On the floor
In a drawer
17
What is the maximum amount of agar plates that you can stack in the incubator?
2
5
4
3
18
What is one purpose of flame inoculation?
To cool the loop
To sterilize the loop
To ignite the specimen
To heat the media
19
Gram + bacteria are generally more permeable to most common antibiotics due to lacking an outer membrane and LPS.
False
True
20
What is the role of nutrient broth in motility testing?
To sterilize equipment
To support growth
To enhance color
To cool samples
21
How does antibiotic resistance occur?
With more oxygen
By cooling down
Through mutation
By using more drugs
22
Antimicrobial disks should be placed not closer than ____ in between and from the edge to avoid overlapping the ZOI.
5mm-10mm
10mm-15mm
8mm-10mm
2mm-5mm
23
Which method increases carbon dioxide for bacteria?
Flame method
Candle jar method
Agar plate method
Incubator method
24
What does beta-lactamase do?
Increases drug effectiveness
Reduces bacterial size
Deactivates antibiotics
Enhances bacterial growth
25
What is measured when evaluating a culture and sensitivity test?
Zone of inhibition
Number of colonies produced
Concentration of antimicrobials that diffuse
Size of the colonies produced
26
What is NOT a colony characteristic?
Odor
Temperature
Elevation
Shape
27
The wet prep is better over the hanging drop prep because it doesn't dry out as easily.
True
False
28
The quadrant streak method is used to inoculate agar slant tubes.
False
True
29
Which method is used for sensitivity testing?
Candle Jar Method
Bauer-Kirby Disc Method
Colony Count Method
Gram Stain Method
30
How is the zone of inhibition measured?
In centimeters
In millimeters
In liters
In grams
