STEM Investigatory Project

Ensure that the plant material HAS moisture and dirt.

Which of the following must be required for PLANT IDENTIFICATION

What is the OVERALL AIM of this study? What do you want to evaluate, prove. demonstrate, analyze, test, investigate?

Arrange the plant specimens DIAGONALLY

What are the specific things that will be ACCOMPLISHED in this project?

The variable that CHANGES IN RESPONSE (responding variable).

We MUST NOT stress the experimental animals [ lucky them (⁠╥⁠﹏⁠╥⁠) ]

The intermediate PRODUCTS OF METABOLIC REACTIONS catalyzed by various enzymes that naturally occur within cells.

The BRINE SHRIMP LETHALITY BIOASSAY is widely used in the evaluation of toxicity of heavy metals, pesticides, medicines

SAPONINS found in beans interfere with the replication of cell DNA,

High toxicity test of BIOACTIVE CHEMICALS. Based on the killing ability of test compounds on a simple zoological shrimp

UNDERLYING: the reasoning or principle that underlies or explains something, or a statement setting out this reasoning

The phytochemical ALLICIN FROM GARLIC has anti-bacterial properties.

What will be the EXPECTED POSITIVE RESULTS after the conduct of this study?

Work is done on a/an CLOSED bench or a biosafety hood

ISOFLAVONES, found in SOY, imitate human estrogens and help to reduce menopausal symptoms and osteoporosis.

Indigenous or exotic microorganisms causing SERIOUS OR LETHAL diseases through respiratory transmission (Disease control

It is very important to understand that this form of waste remediation uses TOXIC CHEMICALS

Compounds produced by an organism that are NOT REQUIRED FOR PRIMARY METABOLIC processes.

measure of the SOLVENT EFFICIENCY to extract specific components from the original plant material

Where did the IDENTIFIED problem or issue began? HOW SERIOUS is the identified problem?

INDOLES, which are found in CABBAGES, stimulate enzymes that make the estrogen less effective

The process of using ORGANISMS TO NEUTRALIZE or remove contamination from waste

The laboratory work is supervised by an individual with general training in BIOTECHNOLOGY.

What was WRONG, incomplete or weak about the prior efforts?

VERTEBRATE ORGANISMS that are of growing interest for preclinical drug discovery applications

performed by implanting a membrane containing the compound of interest on the CHICK EMBRYO chorioallantoic membrane

The subsequent incubation period ranges from 1-3 DAYS.

What METHODS WILL YOU USE in order to address your research problems?

Always COLLECT SAMPLES in an area with sufficient plant material.

An enclosed, VENTILATED LABORATORY workspace for safely working with materials contaminated.

the EXTRACTION of an essence or active ingredient from a substance by boiling

Defined and characterized strains of microorganisms that pose little to NO THREAT OF INFECTION in healthy adults

Associated with MILD HUMAN DISEASES with moderate potential hazards (Centers for Disease Control and Prevention).

If death was the result of a experimental procedure, the study MUST BE TERMINATED.

Tumbler's dark color attracts the FEMALE MOSQUITO the moist soaked lawanit paddle serves as a conducive site Deposit egg

A gross, but simple explanation of bioremediation is the use of MAGGOTS in wound care control

The variable that is purposely CHANGED OR MANIPULATED

Procedures PERFORMED ON LIVING ANIMALS for purposes of research into basic biology and diseases

Why is this PROJECT IMPORTANT? What will be the contribution and innovative application of this research project?

What are the HAZARDS/RISKS factors involved in the study?

The INSTITUTIONAL ANIMAL OVERSIGHT review and approval body for all animal studies at a Regulated Research Institution

LABEL the dry specimens

...AND THEIR CONTRIBUTION TO RESEARCH.

...THE EXPERIMENTAL PROTOCOL to minimize pain or distress to the animals

The number of DEAD LARVAE counted after six days from installation is taken as an index to the effectiveness of the trap

EXTRACTION, SCREENING and IDENTIFICATION of the medicinally active substances found in plants.

A/an AUTOCLAVE should be readily available for decontaminating waste materials.

QUICK, technically SIMPLE, and INEXPENSIVE. A major drawback is that it is labor intensive due to the large no. of eggs

Have generally the shared BIOLOGICAL PURPOSES across all species.

The treatment groups or trials that receive all of the SAME CONDITIONS

The ___ can be a drug, a biochemical substance or organic sample.

What is the SPECIFIC AND TESTABLE assumption that the research data will be expected to support?

receives the exact treatment as the experimental group except it DOES NOT RECEIVE THE CHANGE of the independent variable

DECONTAMINATION can be achieved by treating with chemical disinfectants or by steam autoclaving.

Which of the following are required for Project Background Research?

The plant parts were BOILED in a specified volume of water for a definite time. It

The maximum permissible weight loss or growth retardation (compared to controls) of any experimental animal is 20%

...VERTEBRATE ANIMALS WITH INVERTEBRATES, lower life forms, tissue/cell cultures whenever possible.

An investigative procedure in laboratory medicine, pharmacology, environmental biology and molecular biology for measuri

The OL-TRAP is a simple device made up of a black plastic tumbler, lawanit paddle and mosquito pellets.

(A) one variable can be tested / (B) it is clear how the changes will be measured / (C) it includes a prediction

Phytochemicals have ANTIOXIDANT ACTIVITY and protect our cells against oxidative damage and reduce the risk of cancer

Zebrafish embryos develop most of the major organ systems present in mammals in >1 week

...THE NUMBER OF ANIMALS without compromising statistical validity

The following are examples of VERTEBRATE ANIMALS EXCEPT:

Which of the following is INCORRECT about Phytochemicals?

The test has been used widely to study TOXICITY OF MANY PESTICIDES

an efficient test for chemical screening and IN SITU MONITORING for toxicity of environmental contaminants

Some phytochemicals BIND PHYSICALLY TO CELL WALLS thereby preventing the adhesion of pathogens to human cell walls

SYNTHESIZED BY THE CELL because they are indispensable for their growth

.....depending on the compound, after which time ANGIOGENESIS can be quantified via image analysis

Usually SYNTHESIZED IN PLANTS for particular needs

___ is not crucial for normal living cells in normal GROWTH, DEVELOPMENT, AND REPRODUCTION.

What will be the MATERIALS, EQUIPMENT, apparatuses, chemicals and test organisms that will be used in the study?

The SEPARATION of medicinally active portions of plant tissues

Dangerous microorganisms posing high risk infections which are OFTEN FATAL and without treatment or vaccines