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BACE Practice Exam Review
BACE Practice Exam Review
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Review for the BACE Exam #crhs #bace #biotechnology #review
Flashcards
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Questions
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Answers
1
What is the SI unit for temperature?
Celsius
Fahrenheit
Erlenmeyer
Kelvin
2
Organism with no nucleus or membrane bound organelles; its DNA is contained in the cytoplasm
Plasmids
Prokayotes
Eukaryotes
Biotechnology
3
An aqueous solution and yields a hydrogen ion when ionized is
a buffer
a base
an acid
molarity
4
The order of amino acids on a polypeptide chain is encoded by:
mRNA
DNA
Regulatory proteins
rRNA
5
Using the scientific method, which step would you normally perform
first
?
Develop a testable hyptothesis
State a scientific question based on information/observation
Plan a valid experiment
Formulate a conclusion based on data and error analysis
6
In the steps of the pathway of launching a new biopharmaceutical drug, which step would be
last
Development of an idea for a product/Product Identification
Research and Development
FDA Approval
Sales and Marketing
7
How are the proteins in fractions collected during column chromatography visualized?
Using agarose gel electrophoresis
Using polyacylamide gel electrophoresis
Using a pH meter
Using microscopy
8
If a solution has a pH reading of 7, it is...
special
acidic
basic
neutral
9
Well-substantiated explanation of an aspect of the world: using facts, laws & tested hypotheses
Hypothesis
Theory
Law
10
How much more acidic is a pH reading of 4 versus a pH reading of 5?
1 time more acidic
10 times more acidic
10000 times more acidic
100 times more acidic
11
DNA replication occurs 3' to 5'
True
False
12
In aerobic respiration carbohydrates are ultimately broken down into:
Acetyl-CoA, H2O, ATP
Heat
CO2, H2O, ATP
O2, H2O, ATP
13
How many ATP are produced by glycolysis and fermentation?
2
4
38
36
14
Meiosis occurs in all sexually reproducing eukaryotes
True
False
15
Food & Drug Administration (FDA) regulations can be found in:
The Code of Federal Regulations
The U.S. Rule Guide
The U.S. Pharmacopeia
The Code of Federal Activities
16
Cells produced by mitosis are haploid
False
True
17
The two strands of DNA that constitute the double helix are joined by
ionic bonding
covalent bonding
hydrophobic interactions
hydrogen bonding
18
DNA polymerases are the enzymes that create DNA molecules by assembling nucleotides
false
true
19
Using the scientific method, which step would you normally perform
last
?
Formulate a conclusion based on data and error analysis
Develop a testable hyptothesis
Plan a valid experiment
State a scientific question based on information/observation
20
Organism containing a nucleus and other organelles enclosed within membranes
Plasmids
Biotechnology
Prokayotes
Eukaryotes
21
Type of test used to measure the amount of total protein present
Concentration Assay
ELISA
Activity Assay
Potency Assay
22
DNA polymerases from the thermophiles are used in polymerase chain reation.
false
true
23
Which department in a biopharmaceutical company is looks to identify future products?
Manufacturing
Quality Assurance
Sales and Marketing
Research and Development
24
How can you determine the molecular weight of a substance?
Use a periodic table.
All of the above are viable options.
Look at the original bottle.
Look it up on the internet.
25
Type of test that demonstrates whether or not an enzyme or drug is having the desired effect
Concentration Assay
Potency Assay
ELISA
Activity Assay
26
Which of the following is an mRNA codon?
Tyrosine
UAU
ATA
All of the above
27
A document listing the detailed steps of key processes to help ensure consistent output.
Loading dye
Standard Operating Procedures (SOP)
Batch Record
ELISA
28
Lipids provide which of the following functions for cells?
Genetic code
Structure
Stored energy
Transportation
29
Descriptive generalization of how some aspects of the world behaves under stated circumstances
Theory
Hypothesis
Law
30
DNA is replicated on the leading and the lagging strands in exactly the same way.
false
true
31
The deliberate linkage of DNA from different sources into one molecule
Recominant DNA
Biotechnology
Genetic engineering
Gene therapy
32
Autonomously replicating small circles of DNA present in some bacteria
Eukaryotes
Genetic Engineering
Biotechnology
Plasmids
33
What is a product of glycolysis?
All of the choices are correct
NADH
Pyruvate
ATP
34
In the steps of the pathway of launching a new biopharmaceutical drug, which step would be
2nd?
Sales and Marketing
Development of an idea for a product/Product Identification
Research and Development
FDA Approval
35
When inserting a gene into a plasmid to use as a therapeutic protein, which would be step 1?
Use ligase to "paste" the gene into the plasmid
Transform the bacteria with the plasmid containing gene
Digest the amplified gene PCR product w/ restriction enzyme
Design primers to amplify the gene using PCR
36
In the steps of the pathway of launching a new biopharmaceutical drug which step would be
first
Research and Development
Development of an idea for a product/Product Identification
Sales and Marketing
FDA Approval
37
The separation of molecules, on or thought, a stationary phase is called:
Translation
Chromatography
Electrophoresis
Transcription
38
Which department is in charge of testing a biopharmaceutical product to ensure safety/potency
Manufacturing
Research & Development (R&D)
Quality Control (QC)
Quality Assurance (QA)
39
If a solution has a pH reading of 3, it is more
basic
neutral
special
acidic
40
Which of the following are
NOT
correctly paired?
Nucleic Acids: Maltose
Proteins - Enzymes
Lipid - Cholesterol
Carbohydrate - Cellulose
41
In molecular biology, DNA is _________ into RNA, which is __________ into protein.
translated, transcribed
transcribed, translated
transfected, translated
transducted, transcribed
42
What is the purpose of SDS when used in SDS page electrophoresis?
Stain the proteins for visualization
Denature the proteins & give them a negative charge
Use as a loading dye
Conduct electricity
43
During meiosis, chromosomes may "cross-over" and shuffle their genetic information.
False
True
44
Most ATP in eurkaryotic cells is produced in the..
Cytoplasm
Rough ER
Nucleus
Mitochondria
45
In a biotechnology lab, tap water is best used for:
The final rinse when washing glassware
All of the above
The first rinse when washing glassware
Making buffers
46
If you are to transfer 11 mL of a solution into a 15 mL cylinder. Which pipette will you use?
10 ul micropipette
100 mL macropipette
100 ul micropipette
10 mL macropipette
47
Using the scientific method, which step would you normally perform
second
?
Develop a testable hyptothesis
Plan a valid experiment
Formulate a conclusion based on data and error analysis
State a scientific question based on information/observation
48
The final electron acceptor in aerobic respiration is...
Oxygen
Water
Carbon Dioxide
NAD+
49
A chemicals MSDS is also known as:
Medical Safety Data Sheet
Medical Solution Data Sheet
Material Safety Detail Sheet
Material Safety Data Sheet
50
If a solution has a pH reading of 9, it is more
basic
neutral
acidic
special
51
Where does translation occur in prokaryotes?
in the endoplasmic reticulum
in the nucleus
outside the cell
at the ribosomes
52
A solution that resists changes in pH
an acid
molarity
a base
a buffer
53
Most ATP in eukaryotic cells is produced in the
Cytoplasm
Mitochondria
Rough endoplasmic reticulum
Nucleus
54
What is agar?
Solid media on which bacteria grow
Solid matrix used for DNA gel electrophoresis
Liquid media for bacterial growth
Solid matrix used for vertical gel electrophoresis
55
The FDA requires biopharmaceutical companies to follow current good manufacturing practices.
True
False
56
Meiosis is a type of cell division which increase the chromosome number by two
True
False
57
What part of an amino acid differs from one to the other?
Amino group
R group
Carboxyl group
All of the above
58
In a cell, the correct order of assembly is:
Amino acid, protein, polypeptide
Protein, amino acid, polypeptide
Polypeptide, amino acid, protein
Amino acid, polypeptide, protein
59
In glycolysis, the activation of glucose is accomplished by what?
ATP
Carbon Dioxide
Coenzyme A
Oxygen
60
What type of bond is H
2
O?
Polar covalent
Ionic
Nonpolar covalent
None of the choices are correct
61
Which type of DNA have linear, long pieces with many thousands of genes located in a nucleus?
Eukaryotic
Plasmid
Prokaryotic
Archaea
62
The use of living systems or organisms to develop or make useful products
Recominant DNA
Gene therapy
Genetic engineering
Biotechnology
63
The study of decision making as it applies to morals regarding advances in biotechnology is
biotechnolgy
The U.S. Rule Guide
quality assurance
bioethics
64
A tentative statement about the world leading to deductions that can be tested.
Law
Theory
Hypothesis
65
Germinating or sprouting plants are actively undergoing mitosis
False
True
66
How do DNA molecules vary from one species to another?
The way nitrogenous bases pair
The # of genes & non-coding regions
The directionality of the strand
The type of nitrogenous bases they contain
67
Parkinson's, diabetes & cystic fibrosis (CF) are examples of
Gene therapy
Recombinant DNA
Genetic disorders
Translation
68
Most ATP produced during aerobic respiration occurs during...
Chemiosmosis
The Krebs Cycle
Substrate-Level Phosphorylation
Glycolysis
69
Pyruvate is metabolized when no oxygen is present through the process of?
Oxidative Phosphorylation
Kerbs Cycle
Aerobic Respiration
Fermentation
70
Which is a monosaccharide commonly used as an energy source for cells in culture media?
Starch
Albumin
Cellulose
Glucose
71
The measurement of the concentration of hydrogen ions in a sample.
pH
an acid
a buffer
molarity
72
Type of test used to measure the amount of active ingredient in a drug.
Activity Assay
ELISA
Concentration Assay
Potency Assay
73
When disposing of an acid what must it be treated as?
None of the above.
Hazardous waste
Biohazard waste
Non-harmful waste
74
Viscous buffer used to track DNA or proteins during electrophoresis
Loading dye
Standard Operating Procedures (SOP)
Batch Record
ELISA
75
In the pathway of launching a new biopharmaceutical, which step would require clinical trials?
5
3
2
1
