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Laboratory Safety, Equipment, and Microscopes Flashcards | StudBud
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Laboratory Safety, Equipment, and Microscopes
Laboratory Safety, Equipment, and Microscopes
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Questions
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Answers
1
Label the Microscope: What is #10
Arm
Aperture diaphram
Stage clips
Stage
2
Blood Collection: What tubes are used for Serum for blood chemistry
Green or tan
Red
Light Blue
Red/Grey tubes
3
Approximately what angle is the angled centrifuge at?
54
48
37
52
4
GOOD labs have/are
Well lit
Located separate from hospital operations (break room, lobby)
Constant room temperature
Large enough to fit equipment
5
Label the Microscope: What is #6
Stage clips
Stage
Aperture diaphram
Arm
6
Label the Microscope: What is #8
Fine Focus
Aperture Diaphragm
Coarse Focus
Light
7
When viewed through a microscope, the image..
Comes as it's portrayed
Is reversed
Is upside down
is upside down and reversed
8
Water baths/heat blocks are used for
Heat warming packs that can be placed on a subject
Reheating up samples
Warming up pets suffering from hypothermia
Keeping smaples in a constant temperture
9
In the image below: What is the Red section?
Plasma/Serum
Packed Red Cells
Wax Plug
Buffy Coat
10
Label the Microscope: What is #9
Ocular Tube
Aperture diaphram
Objective Lens
Eye Piece
11
What is true about the refractometer?
It will be completely blue when there's no sample on
It zooms into the sample
Reads light/dark interface
it measures in g/dL
12
What is true about incubators?
Need humidity
Keeps at a constant 37 degrees celsius
Designed to nurture and accelerate the growth of new organisms
Keeps bloody samples at room temp
13
What is OSHA?
Personal Protective Equipment
Bodily fluids, feces, skin scraping
Eliminate or reduce exposure to a chemical/hazard
Occupational Safety and Health Administration
14
Label the Microscope: What is #7
Fine Focus
Coarse Focus
Light
Aperture Diaphragm
15
Blood Collection: What tubes are used for Plasma for blood chemistry
Light Blue
Red/Grey tubes
Red
Green or tan
16
Once separated, the liquid portion on top is the sediment while the bottom solids are called supernatant. True or false?
False
True
17
What is PPE?
Eliminate or reduce exposure to a chemical/hazard
Occupational Safety and Health Administration
Personal Protective Equipment
Bodily fluids, feces, skin scraping
18
Blood Collection: Red tubes have what addictive?
No additive
Silicon Covered
Sodium Cirate
Gel Separator
19
What is a low power lens?
100x
4x
10x
40x
20
What is a Light microscope?
Electron microscope
Phase- contrast
Compound microscope
Dark field microscope
21
What is a scanning lens?
10x
40x
4x
100x
22
Blood Collection: What tubes are used for Hematology?
Red/Grey tubes
Lavender, royal blue, or tan
Green or tan
Grey
23
Blood Collection: Lavender, royal blue, or tan tubes have what addictive?
Gel Separator
Hepartin
EDTA
Clot Activator
24
Blood rockers are the same as a centrifuge, just separates at a slower pace. True or False?
False
True
25
Reagents are a substance or mixture use in a chemical reaction. True or false?
True
False
26
Palsma should make of _ of whole blood
45%
50%
55%
60%
27
Blood Collection: Red with grey tubes have what addictive?
Hepartin
EDTA
Clot Activator
Gel Separator
28
What is a biohazard?
viruses/bacteria that are carried in blood & can cause disease
Written program that helps prevent people from, hazardous chemicals
Substances that pose a threat to humans/animals
Contains info on potential hazards and how to work safely w/ chemicals
29
What is a Chemical Hygiene Plan?
Eliminate or reduce exposure to a chemical/hazard
viruses/bacteria that are carried in blood & can cause disease
Contains info on potential hazards and how to work safely w/ chemicals
Written program that helps prevent people from, hazardous chemicals
30
A refractometer measures
Gravity in urine
Liquid consistency
Total solids
Total proteins in blood
31
What do centrifuges do?
Measure concentration
Measure gravity
Measure total solids
Spin samples at high speeds
32
How do you care for a microscope when you're done with it?
lower stage
turn off light
Rotate nosepiece to low power objective lens
Cover microscope
33
What is a Microhematocrit tube used for?
Concentration levels
Measure PCV
34
You can break a centrifuge when..
only if the equipment is malfunctioning
you think the samples are ready
to put in more samples
to switch into another centrifuge
35
The substage condenser..
Opens and closes to control amount of light illuminating the object
Focus light from the light source on the object
Moves back and forth, and left to right
Adjusts focus
36
Which is true about Angled centrifuges?
It's also known as the swinging arm
Swings tubes out in a horizontal position
Has drilled in holes at a fixed angle
Spins at higher speeds
37
Label the Microscope: What is #12
Light
Fine Focus
Coarse Focus
Aperture Diaphragm
38
What is the difference between plasma and serum?
Serum is a liquid, plasma is not
Plasma has clotting factors
Serum has clotting factors
There is no difference
39
In the image below: What is the yellow section?
Plasma/Serum
Packed Red Cells
Wax Plug
Buffy Coat
40
What is are Engineering Controls?
Contains info on potential hazards and how to work safely w/ chemicals
Bodily fluids, feces, skin scraping
Eliminate or reduce exposure to a chemical/hazard
SDS
41
Blood Collection: Grey tubes have what addictive?
No additive
Potassium Oxalate
Sodium Citrate
Sodium Fluoride
42
Label the Microscope: What is #11
Arm
Stage
Aperture diaphram
Stage clips
43
Blood Collection: light blue tubes have what addictive?
Sodium Cirate
EDTA
Gel Separator
Silicon Covered
44
In the photo below: what part is #3
Calibration Bolt
Eye piece (Diopter Adjustment Ring)
Cover
Refractive Prism
45
Label the Microscope: What is #4
Aperture diaphram
Eye Piece
Ocular Tube
Objective Lens
46
In the photo below: what part is #4
Eye piece (Diopter Adjustment Ring)
Optical System Piping
Cover
Calibration Bolt
47
A large buffy coat can mean
Infection
Not enough amount of sample
Not spun fully
Clotting disorder
48
In the photo below: what part is #6
Eye piece (Diopter Adjustment Ring)
Optical System Piping
Calibration Bolt
Cover
49
Binocular heads are used...
only for teaching
Nearly for all routine labs
Single use
To examine a subject closer
50
What is the typical magnification in the ocular of a compound light microscope?
x4
x10
x40
x100
51
The Aperture diaphragm..
Focus light from the light source on the object
Opens and closes to control amount of light illuminating the object
Adjusts focus
Moves back and forth, and left to right
52
All labs should have what present?
Sink
Electrical supply
Storage
Internet access
53
Blood Collection: which tubes have anticoagulant factors?
Grey
Light Blue
Green or Tan
Lavender, royal blue, or tan
54
Which is true about Horizontal head centrifuges?
It's also known as the swinging arm
Swings tubes out in a horizontal position
Spins at higher speeds
Has drilled in holes at a fixed angle
55
What is true about a CHO
a Chemical Hygiene Officer
OSHA requires one person to be the CHO
A person who is responsible for implementing the CHP
56
What is a Bloodborn Pathogen?
Written program that helps prevent people from, hazardous chemicals
Substances that pose a threat to humans/animals
viruses/bacteria that are carried in blood & can cause disease
Contains info on potential hazards and how to work safely w/ chemicals
57
Blood Collection: What tubes are used for Coagulation Studies
Light Blue
Green or tan
Red/Grey tubes
Red
58
What happens if a centrifuge is ran for too long or too fast?
May rupture cells or destroy features of the cells in the sediment
Nothing
Samples get remixed
The centrifuge can burn out
59
Less plasma means
dehydrated
low white blood cells
low proteins
anemic
60
How do you use a refractometer?
Just look into it
Look into it in a dark room
Put a flash of light on it
Look up at a light sorce
61
What are two types of test tubes we use?
Microhematocrit Tubes
Centrifuge Tubes
Conical Tubes
Capillary Tubes
62
In the image below: What is the middle bottom section?
Plasma/Serum
Buffy Coat
Wax Plug
Packed Red Cells
63
What is the total magnification of a microscope wit x10 ocular and 40x objective?
40x
4x
10x
400x
64
In the photo below: what part is #5
Calibration Bolt
Cover
Optical System Piping
Eye piece (Diopter Adjustment Ring)
65
Label the Microscope: What is #1
Aperture diaphram
Objective Lens
Eye Piece
Ocular Tube
66
In the photo below: what part is #2
Refractive Prism
Eye piece (Diopter Adjustment Ring)
Calibration Bolt
Cover
67
Blood Collection: Green or tan tubes have what addictive?
EDTA
Clot Activator
Gel Separator
Hepartin
68
Automatic analyzers can be used for
Coagulation
Clinical chmistry
Hematology
Electrolytes
69
The stage..
Adjusts focus
Opens and closes to control amount of light illuminating the object
Moves back and forth, and left to right
Focus light from the light source on the object
70
In the image below: What is the middle white section?
Buffy Coat
Plasma/Serum
Packed Red Cells
Wax Plug
71
Blood Collection: What tubes are used for Glucose and coagulation testing?
Red/Grey tubes
Lavender, royal blue, or tan
Grey
Green or tan
72
What is true about a SDS
Only accessible to employees who come into contact with hazardous chemicals
Is accessible at all times to all employees
It is only accessible when an emergency occurs
A memory card containing a patient's files
73
Compound Light Microscope...
generates image by using a combination of lenses
generates image by using light reflection
74
In regards to PPE, all labs should have
Lab coats
Gloves
Eyewear
Ear plugs
75
What MINIMAL equipment does a lab need?
Refractometer
Microhematocrit Centrifuge
Clinical Centrifuge
Microscope
76
What is Zoonoses?
Occupational Safety and Health Administration
Leptospirosis, Brucellosis, Babesiosis- parasitic infection RBC's
Personal Protective Equipment
Transmittable agents from animals to human
77
What is a high dry lens?
40x
10x
4x
100x
78
What is a oil immersion lens?
4x
100x
40x
10x
79
What is a Safety Data Sheet?
Contains info on potential hazards and how to work safely w/ chemicals
Eliminate or reduce exposure to a chemical/hazard
SDS
Bodily fluids, feces, skin scraping
80
Microhematocrit hold type types of tubes
Conical Tubes
All test Tubes
Microhematocrit tubes
Capillary tubes
