What is a DNA/mRNA vaccine?

A vaccine using genetic material

Crops with inserted foreign genes

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Molecular Biology Basics

27 termsStudBud Administration

Explore the fundamental concepts and techniques in molecular biology with this quiz

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Questions 27

Answers

1
True or false: Insulin is produced by recombinant E. coli.
True
False
2
What is the role of fluorescent dyes in gel electrophoresis?
To amplify DNA
To visualize DNA
To separate DNA
To cut DNA
3
What is the role of Taq polymerase in PCR?
To separate DNA
To visualize DNA
To add nucleotides
To cut DNA
4
In gel electrophoresis, which fragments move faster?
All fragments
Larger fragments
Equal size fragments
Smaller fragments
5
What is a common concern about GM crops?
Human health safety
Cost of production
Plant growth rate
Soil quality
6
What is the main use of RT-qPCR?
To amplify DNA
To cut DNA
To visualize proteins
To measure RNA levels
7
What does PCR stand for?
Polymerase cycle reaction
Protein chain reaction
Polymerase cloning reaction
Polymerase chain reaction
8
What is a DNA/mRNA vaccine?
A live virus vaccine
A protein-based vaccine
A vaccine using genetic material
A traditional vaccine
9
What is electroporation used for?
To introduce DNA into cells
To visualize DNA
To amplify DNA
To cut DNA
10
What does homologous recombination do?
Amplifies DNA
Inactivates genes
Cuts DNA
Introduces new sequences
11
What does CRISPR/Cas9 do?
Cuts and alters DNA
Amplifies DNA
Visualizes DNA
Creates RNA
12
What is recombinant DNA?
DNA from two sources
DNA from bacteria
DNA from one source
DNA from viruses
13
What is the significance of sticky ends in DNA?
They visualize DNA
They cut DNA
They help in ligation
They amplify DNA
14
Which polymerase is heat-stable?
Taq polymerase
RNA polymerase
DNA polymerase I
Reverse transcriptase
15
What do stem cells do?
Amplify DNA
Create proteins
Cut DNA
Regenerate tissues
16
What is the main goal of genome editing?
To modify genes precisely
To amplify DNA
To visualize DNA
To cut proteins
17
What is a knockout mouse?
A mouse with a disabled gene
A mouse with no genes
A mouse with modified proteins
A mouse with extra genes
18
What is a transgenic organism?
An organism with foreign genes
An organism with mutations
An organism with no genes
An organism with altered proteins
19
What is the first step in PCR?
Extension
Cooling
Annealing
Denaturation
20
What is the purpose of a guide RNA in CRISPR?
To direct Cas9 to DNA
To visualize RNA
To amplify DNA
To cut DNA
21
What are sticky ends?
Single-stranded overhangs
Double-stranded ends
Circular DNA pieces
DNA fragments
22
What is the purpose of gel electrophoresis?
To separate DNA fragments
To visualize RNA
To cut DNA
To amplify DNA
23
RT-qPCR measures levels of what?
RNA
DNA
Cells
Proteins
24
What is a successful application of stem cell therapy?
Gene editing
Protein production
Bone marrow transplant
DNA amplification
25
Which enzyme cuts DNA at specific sites?
Restriction endonuclease
Taq polymerase
Gel electrophoresis
DNA polymerase
26
What guides Cas9 to DNA?
Restriction enzyme
DNA polymerase
Taq polymerase
Guide RNA
27
What are GM crops?
Crops with extra nutrients
Crops with altered proteins
Crops with no genes
Crops with inserted foreign genes

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