Duplicate of Introductory Micro Lab Exam 1

Name of organism, student name, time of day and date are included when labeling a plate or a test tube.

Low power lens has smallest field of view

What gram reaction would you expect if you used the endospore decolorizer by accident?

The results shown here are the results of

This is an example of too much overlapping

Oil immersion lens has largest field of view

Heat used during the endospore staining procedure helped stain to penetrate the spore coat

Procedures that involve aseptic technique include:

Identify the morphology of this specimen

What color would you expect the endospores to be if you performed an acid-fast staining on endospore +ve bacteria

What is the Acid-fast reaction of the bacterium shown here?

Fine focus is used to focus when using the oil immersion lens

The ability to distinguish between objects

In order to obtain a pure bacterial culture, what is a quick and easy technique?

Not everything used in aseptic technique needs to be sterile

The loop should be flamed and then immediately used to pick up bacteria in order to avoid contamination

A false-negative Gram reaction might occur if

Course focus is used to focus when using the scanning lens

In pour plate method few colonies may grow on the surface.

Which of the following step is necessary to prevent rupturing of cells during smear preparation.

Wearing closed shoes is a part of aseptic technique in preparing for lab

550X total magnification is achieved with the

Which of the following conditions might result in a false-negative endospore staining result?

Bacterial smear first be allowed to air dry before any other step in smear preparation and staining?

A false-positive Gram reaction might occur if too much bacteria are on the slide

What gram reaction would you expect if you used the acid-fast decolorizer by accident?

Pour plate technique is often used to identify the bacteria quickly

What bacterial species was used in the acid-fast staining lab?

Condenser Focuses light on the specimen

You should always begin viewing a slide under low power or scanning because the field of view is larger

In pour plate method the embedded colonies -------

What is the most likely mistake made in the picture?

What is the morphology of the bacteria in the stain?

You have never used a parfocal microscope in this lab course.

What was used as a mordant for acid-fast staining?

Which of the following staining will reveal the cell wall composition?

The purpose of a pour plate is to count the bacteria.

The decolorizer for the Acid-fast staining is

Preparation for the pour plate method is time-consuming compared with the streak plate/and or spread plate technique.

Lysozyme is an example of a non-specific antimicrobial system that is most effective in killing this type of bacteria?

The following structure controls the amount of light on the specimen

When streaking several similar plates, it is not important to flame the loop between streaks.

What is the Gram reaction of the bacterium shown here?

Which of the following samples would you perform an acid-fast stain on?

Cerebral spinal fluid from a patient suspected of having meningitis will not have any organism

Which of the following is a part of aseptic technique in preparing for lab?

What cell wall material is indicated by this gram reaction?

400X total magnification is achieved with the High Power lens

Which of the following are medically important endospore producing organisms?

Basic stains have positive charge.

During the streak plate procedure, culture is taken

In light compound microscope the Scanning lens has the shortest working distance

Which of the following bacterium may demonstrate a false negative endospore result?

Aseptic technique means using practices and procedures to prevent contamination from pathogens.

Albumin is used in the lysozyme activity for this purpose?

A quick pass of the smear through heat will allow bacteria to adhere with the glass surface

What type of reaction is this?

Which of the following is used in negative staining procedure

The lens with the shortest working distance is oil immersion lens

Which of the following are basic stains

The stain used for this slide was acidic stain

The pour plate method requires less skill compared to streak plate method.

In performing Kirby-Bauer method of testing the zone sizes are looked up on a standardized chart to give a result of....

70X total magnification is achieved with the …

Because the specimen remains in focus when changing objectives

What is the Gram reaction of the bacterium shown here?

When streaking a plate, it is important to flame the loop between streaks.

Air drying of smear will increase the absorption of dye

This is coccus

Which of the following is correct?

The distance between the objective and the slide is called working distance

100X total magnification is achieved with the

In Endospore staining the endospore were stained pink

What is the most likely mistake that was made when preparing this steak plate?

The stain used for this slide was a basic stain