What is the field of bioinformatics?

Compiling, searching & analyzing sets of DNA sequences

In a microarray experiment, what is the purpose of reverse transcriptase?

to make cDNA target molecules from different cells

If the negative control spot of a microarray gave a signal, which is a likely explanation?

all of these could be an explanation, the wash step was ineffective, the temperature of hybridization was too cold, the chip was prepared incorrectly

Why does the gene for actin beta make a good positive control for a microarray?

it is expressed in every living cell

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Microarray & Genomics Review

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Review for West Biotech exam on microarray technology #biotechnology

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Answers

1
What is the field of bioinformatics?
Creating artificial intelligence using DNA
Merging of biological organisms with mechanical parts
Compiling, searching & analyzing sets of DNA sequences
Using text messages to communicate with other life forms
2
Which process assembles DNA using an RNA template?
transcription
reverse transcription
hybridization
translation
3
The field of genomics uses all of the following except:
studying all the genes of an organism simultaneously
Studying a single gene
using model organisms
using computers to process huge amounts of data
4
Which of the following information is NOT part of a Gene’s Ontology?
molecular function of the protein
location of the gene
location of the protein
biological process of the protein
5
In a microarray experiment, what is the purpose of reverse transcriptase?
to speed up the process of hybridization of target to probes
to make cDNA target molecules from different cells
to make the DNA probes that will be stuck on the chip
to make RNA that is complementary to probe DNA on the chip
6
If the negative control spot of a microarray gave a signal, which is a likely explanation?
all of these could be an explanation
the wash step was ineffective
the temperature of hybridization was too cold
the chip was prepared incorrectly
7
Portions of messenger RNA that are removed before translation are called
promoters
exons
terminators
introns
8
The study of all the genes of an organism simultaneously is
gene simmons
genomics
genetics
geneaology
9
Why does the gene for actin beta make a good positive control for a microarray?
it is expressed in lung cells, but not plant cells
it is a catalyst for RNA transcription
it is expressed in every living cell
it is only active during fetal development
10
Which process assembles RNA using a DNA template?
reverse transcription
hybridization
transcription
translation

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