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BACE Practice Exam Review
BACE Practice Exam Review
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Review for the BACE Exam #crhs #bace #biotechnology #review
Flashcards
74 cards
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Questions
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Answers
1
What is agar?
Solid media on which bacteria grow
Solid matrix used for vertical gel electrophoresis
Liquid media for bacterial growth
Solid matrix used for DNA gel electrophoresis
2
How many ATP molecules are produced by glycolysis and fermentation?
4
22
2
1
3
A chemical's MSDS is also known as:
Medical Safety Data Sheet
Material Safety Data Sheet
Medical Solution Data Sheet
Material Safety Detail Sheet
4
Type of test used to measure the amount of total protein present
Concentration Assay
Activity Assay
Potency Assay
ELISA
5
Which department is in charge of testing a biopharmaceutical product to ensure safety/potency
Quality Assurance (QA)
Quality Control (QC)
Research & Development (R&D)
Manufacturing
6
How much more acidic is a pH reading of 4 versus a pH reading of 5?
10 times more acidic
10000 times more acidic
100 times more acidic
1 time more acidic
7
Organism with no nucleus or membrane bound organelles; its DNA is contained in the cytoplasm
Prokayotes
Plasmids
Eukaryotes
Biotechnology
8
What part of an amino acid differs from one to the other?
Carboxyl group
R group
Amino group
All of the above
9
Most ATP produced during aerobic respiration occurs during...
Chemiosmosis
The Krebs Cycle
Substrate-Level Phosphorylation
Glycolysis
10
What is a product of glycolysis?
Pyruvate
NADH
All of the above
ATP
11
When inserting a gene into a plasmid to use as a therapeutic protein, which would be step 1?
Transform the bacteria with the plasmid containing gene
Digest the amplified gene PCR product w/ restriction enzyme
Design primers to amplify the gene using PCR
Use ligase to "paste" the gene into the plasmid
12
Food & Drug Administration (FDA) regulations can be found in:
The U.S. Pharmacopeia
The Code of Federal Regulations
The U.S. Rule Guide
The Code of Federal Activities
13
A tentative statement about the world leading to deductions that can be tested.
Hypothesis
Theory
Law
14
When disposing of an acid what must it be treated as?
Non-harmful waste
Biohazard waste
Hazardous waste
None of the above.
15
Well-substantiated explanation of an aspect of the world: using facts, laws & tested hypotheses
Hypothesis
Law
Theory
16
Lipids provide which of the following functions for cells?
Transportation
Genetic code
Stored energy
Structure
17
The study of decision making as it applies to morals regarding advances in biotechnology is
The U.S. Rule Guide
quality assurance
biotechnolgy
bioethics
18
Most ATP in eurkaryotic cells is produced in the..
Mitochondria
Nucleus
Rough ER
Cytoplasm
19
What are the most common molecules found in cells?
DNA
Lipids
Carbohydrates
Proteins
20
In the steps of the pathway of launching a new biopharmaceutical drug, which step would be
last
FDA Approval
Research and Development
Sales and Marketing
Development of an idea for a product/Product Identification
21
Cells produced by mitosis are haploid
True
False
22
If a solution has a pH reading of 3, it is more
special
acidic
neutral
basic
23
Germinating or sprouting plants are actively undergoing mitosis
False
True
24
DNA is replicated on the leading and the lagging strands in exactly the same way.
true
false
25
A chemical used in an experiment.
Agar Medium
Just some stuff
Reagent
ATP
26
The order of amino acids on a polypeptide chain is encoded by:
DNA
Regulatory proteins
mRNA
rRNA
27
Autonomously replicating small circles of DNA present in some bacteria
Biotechnology
Genetic Engineering
Plasmids
Eukaryotes
28
In glycolysis, the activation of glucose is accomplished by what?
ATP
Oxygen
Coenzyme A
Carbon Dioxide
29
An aqueous solution and yields a hydrogen ion when ionized is
an acid
a base
molarity
a buffer
30
Type of test that demonstrates whether or not an enzyme or drug is having the desired effect
Concentration Assay
Potency Assay
ELISA
Activity Assay
31
In aerobic respiration carbohydrates are ultimately broken down into:
CO2, H2O, ATP
Acetyl-CoA, H2O, ATP
O2, H2O, ATP
Heat
32
Where does translation occur in prokaryotes?
in the nucleus
outside the cell
at the ribosomes
in the endoplasmic reticulum
33
Descriptive generalization of how some aspects of the world behaves under stated circumstances
Law
Theory
Hypothesis
34
If a solution has a pH reading of 7, it is...
basic
neutral
acidic
special
35
How are the proteins in fractions collected during column chromatography visualized?
Using agarose gel electrophoresis
Using a pH meter
Using polyacylamide gel electrophoresis
Using microscopy
36
Using the scientific method, which step would you normally perform
first
?
Plan a valid experiment
State a scientific question based on information/observation
Develop a testable hyptothesis
Formulate a conclusion based on data and error analysis
37
Using the scientific method, which step would you normally perform
last
?
State a scientific question based on information/observation
Formulate a conclusion based on data and error analysis
Plan a valid experiment
Develop a testable hyptothesis
38
Parkinson's, diabetes & cystic fibrosis (CF) are examples of
Translation
Genetic disorders
Gene therapy
Recombinant DNA
39
What is the SI unit for temperature?
Kelvin
Celsius
Fahrenheit
Super Hot!!
40
The use of living systems or organisms to develop or make useful products
Recominant DNA
Genetic engineering
Biotechnology
Gene therapy
41
In the pathway of launching a new biopharmaceutical, which step would require clinical trials?
3
2
1
5
42
Type of test used to measure the amount of active ingredient in a drug.
ELISA
Concentration Assay
Potency Assay
Activity Assay
43
Which of the following is an example of a product easily found in nature?
Recombinant DNA
GMO
All of the above
Antibiotic
44
The separation of molecules on or through a stationary phase is
Potency Assay
Chromatography
Translation
Electrophoresis
45
Which is a monosaccharide commonly used as an energy source for cells in culture media?
Cellulose
Albumin
Starch
Glucose
46
A solution that resists changes in pH
a base
molarity
a buffer
an acid
47
Which department in a biopharmaceutical company looks to identify future products?
Research and Development
Sales and Marketing
Manufacturing
Quality Assurance
48
In the steps of the pathway of launching a new biopharmaceutical drug, which step would be
2nd?
FDA Approval
Research and Development
Sales and Marketing
Development of an idea for a product/Product Identification
49
The use of an electric field to separate DNA in agarose is called
Bacterial Transformation
Genetic Engineering
DNA Fission
Gel Electrophoresis
50
If you are to transfer 11 mL of a solution into a 15 mL cylinder. Which pipette will you use?
100 mL macropipette
10 ul micropipette
10 mL macropipette
100 ul micropipette
51
The measurement of the concentration of hydrogen ions in a sample.
molarity
pH
a buffer
an acid
52
If a solution has a pH reading of 9, it is more
acidic
basic
special
neutral
53
The various fragment lengths that are separated during electrophoresis are called
RLFPs
Lengths
None of the above
Segments
54
Most ATP in eukaryotic cells is produced in the
Cytoplasm
Rough endoplasmic reticulum
Mitochondria
Nucleus
55
In the steps of the pathway of launching a new biopharmaceutical drug which step would be
first
Research and Development
Sales and Marketing
Development of an idea for a product/Product Identification
FDA Approval
56
During meiosis, chromosomes may "cross-over" and shuffle their genetic information.
False
True
57
The deliberate linkage of DNA from different sources into one molecule
Gene therapy
Recominant DNA
Biotechnology
Genetic engineering
58
What is the purpose of SDS when used in SDS page electrophoresis?
Use as a loading dye
Conduct electricity
Stain the proteins for visualization
Denature the proteins & give them a negative charge
59
Viscous buffer used to track DNA or proteins during electrophoresis
ELISA
Batch Record
Loading dye
Standard Operating Procedures (SOP)
60
Meiosis is a type of cell division which increase the chromosome number by two
True
False
61
DNA replication occurs 3' to 5'
True
False
62
A document listing the detailed steps of key processes to help ensure consistent output.
Loading dye
ELISA
Standard Operating Procedures (SOP)
Batch Record
63
DNA polymerases are the enzymes that create DNA molecules by assembling nucleotides
true
false
64
Meiosis occurs in all sexually reproducing eukaryotes
True
False
65
The FDA requires biopharmaceutical companies to follow current good manufacturing practices.
True
False
66
DNA polymerases from the thermophiles are used in polymerase chain reation.
false
true
67
Which of the following is an mRNA codon?
Tyrosine
UAU
All of the above
ATA
68
Pyruvate is metabolized when no oxygen is present through the process of?
Krebs Cycle
Aerobic Respiration
Fermentation
Oxidative Phosphorylation
69
How can you determine the molecular weight of a substance?
Use a periodic table.
Look at the original bottle.
All of the above are viable options.
Look it up on the internet.
70
In a biotechnology lab, tap water is best used for:
All of the above
The final rinse when washing glassware
The first rinse when washing glassware
Making buffers
71
Organism containing a nucleus and other organelles enclosed within membranes
Eukaryotes
Prokayotes
Biotechnology
Plasmids
72
How do DNA molecules vary from one species to another?
The type of nitrogenous bases they contain
The way nitrogenous bases pair
The # of genes & non-coding regions
The directionality of the strand
73
The moles of solute per liter of solution is...
Acidity
Polarity
Molarity
Density
74
Using the scientific method, which step would you normally perform
second
?
Plan a valid experiment
Formulate a conclusion based on data and error analysis
State a scientific question based on information/observation
Develop a testable hyptothesis