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Medical Interventions Unit 4 Review Flashcards | StudBud
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Medical Interventions Unit 4 Review
Medical Interventions Unit 4 Review
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1
If your job was to create packing for the COVID vaccine that has to stay VERY cold, what profession might you have?
Chemical Engineer
Biomedical Engineer
Marketing Specialist
Materials Scientist
2
Using a pig to create a kidney for human transplantation is
Xenotransplantation
Tissue Engineering
Will never be possible
Animal Engineering
3
Insulin pumps were first available in the 1980s.
False
True
4
By the late 1970's, how was insulin produced?
Bacteria produced with recombinant DNA
Chemically manufactered
Donated by other humans
Harvested from pigs
5
This type of dialysis can be done at home while sleeping in patients with little other health issues
peritoneal dialysis
punctual dialysis
hemodialysis
frengal dialysis
6
Why did they use NaCl in the first well for all the patients?
It would allow us to recognize contamination
It is testing for HLA compatibility
It is testing for high sodium levels in the blood
It is a negative control
7
In the pGLO lab, we added calcium chloride and heat shocked the bacteria to get them to take up the plasmid.
This is translation
This is transformation
This is conjugation
This is transduction
8
In the early 1900's diabetes was diagnosed as sugar in the urine. How was it treated?
Insulin was transfused
Insulin injections were given
High carbohydrate diet
Patients were told to fast
9
Using 'bio inks' to 3D print organs is
xenotransplantation
tissue engineering
the work of materials science
impossible
10
What is the blood type for patient 5 (who has clotting in Anti-Rh well)
B+
O+
B-
A+
11
Why does the +pGLO LB/amp not glow?
There is too much ampicillin to allow translation to occur
It was not transformed
There is no AMP to stimulate the T7 promoter to turn on transcription
There is no IPTG to stimulate the T7 promoter to turn on transcription
12
John-44 yo, 3 kids, single, wealthy on list 5 years Angie- 19, poor, no kids, on list 1 year WHO GETS KIDNEY?
John because he has been on the list longer
John because he can pay for it.
John because he has kids
Angie because she is only 19
13
Which of these would NOT be useful in diagnosing End Stage Renal Disease?
Red Blood Cell Count
GFR (Glomerular Filtrate Rate)
Urinalysis
Liver Function Test
14
If you suspect kidney failure, what test might you order to confirm the diagnosis?
BUN (Blood Urea Nitrogen)
DNA sequencing
ELISA
Blood typing
15
In the GFP or PGlo lab, why did we grow the -pGLO on the LB/amp plate?
To prove that the bacteria didn't already glow.
To prove that the bacteria WERE resistant to ampicillin
To prove that the bacteria were NOT resistant to ampicillin
To prove that the bacteria needed ampicillin to grow
16
Person 1 can be a kidney donor for person 5.
False
True
17
This type of dialysis removes blood through a catheter, filters it and returns it to the body.
hemodialysis
catherized dialysis
peritoneal dialysis
external dialysis
18
Insulin was discovered in 1950.
False
True
19
If you want to isolate the pGLO protein, what is the first step?
Run vertical electrophoresis SDS page
Expose them to Calcium Chloride and heat shock
Place the colonies in a Chromotography column
Use a lysing agent and thaw cycles to remove GFP from cells
20
Which job might a Biomedical Engineer have in the manufacturing of human insulin?
Growing bacteria
Teaching doctors about the new product
Creating the plasmid
Purifying the protein
21
Person 5 can be a kidney donor for person 1.
False
True
22
What is the blood type for patient 1 (who has clotting in anti-A and Anti-Rh wells)
B-
A-
A+
B+
23
In the GFP lab, why did we grow -pGLO on LB?
To prove that the bacteria needed ampicillin to grow
To prove that the bacteria had no IPTG in it
To prove that the bacteria didn't already glow
To prove that the bacteria can undergo conjugation
24
Patients with diabetes might experience FREQUENT URINATION, VISION PROBLEMS and
Kidney failure
Insomnia
Depression
Liver Failure
25
What are chromatography columns used for?
Separate molecules like proteins from other molecules
Verify purity of product
Identify molecules like proteins
26
Which of these would make a patient INELIGIBLE for a kidney transplant?
Having diabetes
No insurance
Under 14 years of age
None of these
27
Sally- 2 year wait, lives 50 miles from donor, recovering drug addict Abel - 2 year wait, lives 1500 miles from donor
Sally because she lives closer
Abel because he is male.
Abel because Sally did drugs
Sally because she is female
28
What is SDS page used for?
Denaturing proteins
Verifying purity of proteins
Separating proteins
Identifying paternity
29
Which of these is a responsibility of the Organ Procurement and Transplantation Network
Test for compatibility of organs
Formulate organ allocation policies and regulations
Keep a list of people awaiting donors and analyze statistics
Transplant organs
