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Clin Path 2: Microbiology Flashcards | StudBud
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Clin Path 2: Microbiology
Clin Path 2: Microbiology
44 terms
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chapter 37-chapter 44 in Sirois
Flashcards
44 cards
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Practice quiz
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Host live
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Questions
44
Answers
1
Which procedure involves the application of undiluted urine directly to the culture and sensitivity test plate?
Direct sensitivity test
Standardized disk sensitivity method
Bauer-Kirby method
Indirect sensitivity test
2
What term describes an organism with growth that requires high levels of carbon dioxide
obligate aerobe
microaerophilic
capnophilic
facultative anaerobes
3
Which is the preferyured culture media used for the growth of fungi and yeasts
Sabouraud agar
TSI
McFarland
New methylene blue
4
Which method is the preferred method when testing for dermatophytes
Make an impression smear of moist underside of scab and examine at 40x mag
Pluck some hairs from the periphery of the lesions and perform a culture
Make a smear of a skin scraping and examine it at 40x magnification
Hold a Wood's lamp over animal and check the skin and coat for fluorescene
5
What structures comprise most fungi
Hyphae
Fruiting bodies
Mycelia
Ascospores
6
Which technique is designed to isolate pure cultures of bacteria?
Slant S streak
Candle jar method
Stab streak
Quadrant streak method
7
Routine cultures are incubated for _____ hours before initial examination
48
72
18
30
8
What is the mordant component in most Gram staining kits
Crystal violet
Iodine
Fuchsin
Safranin
9
#s of colony-forming U on a culture plate of a sampe of urine collected by cysto are signicant when more than ___ U/mL
10
1000
500
100
10
What is the perferred culture media for performing the sensitivity test?
Bauer-kirby
EMB agar
Mueller-Hinton
Blood agar
11
What organisms are primarily identified with the use of acidfast stains
viruses
anaerobes
Pseudomonas
Mycobacterium
12
How or when are mircobiology samples best collected?
Aseptically
Before physical examination
At necropsy
After treatment has started
13
Which term describes the "chinese letter" pattern arrangement of some bacteria
Palisades
Cocci
Bacilli
Pleomorphic
14
What is present when a catalase test is identified as having a postive result
No color change
Clumping on the slide
Bubbles on the slide
Red color at the top of the tube
15
For which test is hydrogen peroxide used as the reagent
Oxidase
Catalase
Coagulase
Indole
16
What term describes an organism with growth that is enhanced in presence of low oxygen tension
capnophilic
microaerophilic
facultative anaerobes
obligate aerobe
17
What is the approximate perferred age of a bacterial culture that is to be subjected to endospore staining?
8
24
48
12
18
Which is a term that can be used to describe the form of a colony on a plate?
Rhizoid
Alpha
Mucoid
Convex
19
The production of which component is detected using Kovac's reagent?
Coagulase
Oxidase
Indole
Catalase
20
What method is used for collection of bacterial samples from hollow organs
swabbing
punch biopsy
imprint
aspiration
21
What method is used to inoculate agar slant tubes?
Candle jar method
Quadrant streak method
Butt stab streak
Slant S streak
22
What term describes an organism with growth that is inhibited in the absence of oxygen
capnophilic
obligate aerobe
facultative anaerobes
microareophilic
23
Which test requires the use of a microscope slide with a concave area?
Wet mount
Hanging drop motility test
SIM test
Kovac's
24
Which procedure involves inoculating the culture and sensitivity test plate with colonies from a pure culture?
Bauer-Kirby
Indirect sensitivity
Standardized disk susceptibility
Direct sensitivity
25
Which organisms are primarily identified with fluroscent stains
Anaerobic bacteria
Mycobacterium
Pseudomonas
Viruses
26
What is the primary stain component is most Gram staining kits
fuchsin
safranin
iodine
crystal violet
27
What type of hemolysis is exhibited by pathogenic bacteria on blood agar
alpha
beta
gamma
no
28
Which stain is primarily used when Gram stain results are ambiguous?
Giemsa
Gram stain
Carbol fuchsin
Ziehl-Neelson
29
Which type of culture media contains additives that allow certain microorganisms to grow while stopping growth of others
selective
differential
enriched
fastidious
30
Which stain is primarily used to detect spirochetes and rickettsiae?
Carbol fuchsin
Giemsa
Gram Stain
Ziehl-Neelsem
31
What is the counterstain component for most Gram staining kits
Iodine
Saftanin
Fuchsin
Crystal violet
32
Which of the following is an example of an area that is likely to contain a single organism
ears
fistulous tracts
urine collected by cystocentesis
fecal samples
33
Which media is recommended for culture and sensitivity testing
Mueller-Hinton
chocolate agar
blood agar
MacConkey
34
Which procedure is performed when Gram stain results are ambiguous
Acid-fast
KOH
Ziehl-Neelsen
DMSO
35
What is the most common cause of diagnostic failure with microbiologic specimens ?
Before physical examination
At necropsy
After treatment has started
Specimen contamination during collection
36
What is the purpose of the candle jar?
Monitor humidity
Sterilize the jar
Increase Carbon Dioxide levels
Create anaerobic conditions
37
What is measured when evaluating a culture and sensitivity test?
Number of colonies produced
Size of the colonies produced
Zone of inhibition
Concentration of antimicrobials that diffuse
38
Which is a useful stain for the evaluation of fungal organisms
Ziehl-Neelsen stain
Endospore stain
Lactophenol cotton blue
Gram stain
39
which term describes rod-shaped bacteria
pleomorphic
bacili
palisades
cocci
40
in which category are most pathogenis bacteria found
psychrophiles
mesophiles
thermophiles
dolicophiles
41
Which type of culture media contains additives that detect certain biochemical reactions of the bacteria
fastidious
selective
enriched
differential
42
What is the first step in collecting samples for dermatophyte testing
Brushing the hair with a toothbrush
Plucking a few skin scales
Cleaning the area to minimize surface contamination
Plucking some hairs
43
How would results be recorded if the milk/reagent mixture in the CMT thickens immediately, with some gel formation
Weak positve
Negative
Distinct postive
Trace
44
What is the preferred location for collection of culture specimens when Microsporum or Trichophyton is suspected
the center of the lesion
the discharge that is draining from the lesion
anywhere on the open surface of the lesion
the periphery of the lesion