Hematology Test 1 Part 1

Most abundant to least abundant:

Thrombocytes stain which color because of Azure B

Reference range for platelets in blood is

Secondary granules to primary granules

EDTA works by binding ______ so platelets cannot initiate clotting.

Cells that have exited cell cycle and enter nondividing phase are said to be in quiescence

Plasma contains water, albumin/proteins, fats and ImG.

The longest phase of cell cycle and most variable stage

The reference range for RBC on the CBC is:

Less color in an RBCs are reflected in this test

Tight, dense and not being transcribed

The white parts seen in basophils are Golgi bodies

The composition of blood is 55% cellular and 45% plasma.

Hypertonic solutions cause cells to shrink or crenate

Romanowsky type stain used in hematology

Horseshoe or bean shaped nucleus, chromatin loose and lacy, grey/blue cytoplasm and may contain vacuoles

Lacey and actively being transcribed to rRNA

Coagulation leads to hemostasis

CBC stands for Computer Based Counts?

MCHC measures average hemoglobin content in RBC

What is hemoglobin?

%of each of the WBCs, WBC estimate, Platelet estimate/morph, RBC morph and WBC Morph are evaluated in a differential

Platelets are thrombocytes

More water on the inside of cell than outside; can cause cell to burst

Most common type of technique used for peripheral blood smear

Cyclin is the regulatory subunit of the active enzyme complex responsible for regulating cell cycle

S phase driven by cyclin A and B

The volume of blood in the average adults is?

What contains hemoglobin and transports O2 and CO2?

Lactoferrin, lysozyme, histamine and heparinase are all examples of primary granules.

3 cellular constituents of blood include:

WBC have nuclei

Cell cycle order

Cyclin D1-3 Drive G1 stage

G2 and M phase driven by

Which protein spans the entire surface of the membrane?

The point at G1 which the cell no longer need growth factors bc commitment has occurred is called

Nucleus has 2-3 loves, granules are large, uniform and pink to orange in color.

MCV,MCH and MCHC are all

RBCs have two proteins needed for the ability to circulate

Central Palor of RBC takes up

Ribosome rich are immature and stain dark blue with Wright stain

All mature RBCs do not have

The ______ is a section of flattened sacs arranged in parallel next to the nucleus

What is the center white area in the RBC called?

Hemostasis is the arrest of bleeding.

Differential can be manual or automated

What is the PREFERRED anticoagulant used in the hematology lab?

Co-factor that helps drive enzymatic activity to help CDK drive cell cycle

Azure B binds to anions in cells including

G0 is the

What is the inactive X chromosome in females that can be seen in a segmented neutrophil

Heparin is natural and neutralizes thrombin

Define differential

Peripheral blood smear is stained with Wright Stain

What are the 3 components of blood?

Blood collection is during which stage:

Sorta, modifies and packages ribosomes from RER

Eosinophils function in parasitic infections and counteract basophil allergic responses.

In Romanowsky stains: nucleus stains purple/blue and nucelolus stains pale blue

RBC, hematocrit and hemoglobin reference ranges can vary because

The presence of this best reflects the maturity of a cell

Ribosomes let us know the cell so actively manufacturing proteins and

The study of blood and blood forming organs

Protein synthesis, growth and phagocytosis are examples of the function of

When stained the ______ cell will let us know the maturity of the cell.

The number and type of cells seen and platelets seen are to determine if there is anything unordinary about the sample

DNA synthesis occurs during the M phase

The reference range for WBC on a CBC is 4.5-11 X 10^9/L

Known functions: “first responder”, phagocytosis, optimizations and is attracted to ImG decorated cell

On a CBC, what is not measured?

Contains DNA which regulates cell function

Apoptosis is orderly while necrosis is not

Plasma membrane loses ability to regulate cations or the rupture of cell membrane

SER produces ribosomes that synthesize large amounts of proteins

What is needed for clotting?

The lab makes a PBS(peripheral blood smear) and does a CBC with coagulated blood.

Which two organelles do not stain under Wright stain

A basic stain that will stain an acidic compounds;ribosomes attracted to acidic compounds

RBC will live for about 120 days

Self inflicted cell death program encoded in DNA of all cells

Most errors occur during the Post-analytical stage.

The main function of a basophil is

Which one is a leukocyte?

What is able to diapedese through tissue?

A CBC (complete blood count) is used for:

Romanowsky effect is due to cationic Azure(basic) B and an ionic Eosin(acidic)Y

Cytoplasm stain blue/purplish

Golgi look like a clear area next to nuclear envelope under a microscope

MCV measures

What are an example of primary granules seen in neutrophils?

Major basics proteins stain pink because they pickup

CD-kinases needed to regulate between cell stages

Nucleus split into 3-5 lobes with dense chromatin, granules present,

On a CBC, what measurement is the portion of blood containing RBCs?

Only found in immature metabolically active cells

Nuclear chromatin can be either

Basophil granules contain

What are the 4 active phases of the cell cycle?

This cell has granules that looks like purple black rocks and are very large.