Micro 101 UNIT 3

Phenol is used in ______ acid fast staining method.

What agar uses crystal violet to inhibit gram positive bacteria?

What should you use to inoculate a slide?

A benefit of using commercial identification systems is?

What is an advantage of disk diffusion testing?

This will stain clear gram negative bacteria pink or red.

Bacteria have nutritional needs found in media. they are either fastidious or non-fastidious.

Ziehl-Neelsen and Kinyoun are two methods of which staining type?

Due to a higher concentration of phenol in the Knyoun acid fast stain _____ is not required.

Under what atmospheric conditions will facultatively anaerobic bacteria grow?

Fluorochrome used to detect mycobacterial cells.

Which of the following are commonly used fluorochromes used in fluorescent microscopy.

What are the reagents in the Gram stain application process?

Which of the following are acid fast staining methods?

Once stained, the smear is examined using the _____ objective.

After_____, organisms that are gram negative are cleared of crystal violet.

Electron beam passed through objects to view internal structures.

When clinical material is gram stained, the slide is evaluated for the presence of________.

Why is Bacitracin is no longer widely used?

Phenylethyl Alcohol agar inhibits which bacteria?

How is Resolution achieved using the 1000x objective in light microscopy?

What two methods can be used to fix a specimen to a slide?

Thioglycollate broth is a non-selective broth for the recovery of?

The use of a microscope to magnify objects too small to see with the naked eye defines.

___________passes electron beams through objects, allowing visualization of internal structures

Light does not pass directly through the specimen but hit the specimen at an oblique angle.

microorganisms taken from a solid media to make a smear should be ______?

The lens closest to the specimen is known as an?

In the Gram stain process, which stain is called the primary stain?

The highest power objective is?

Micoorganisms with long chain fatty (mycolic) acids are able to resist _____.

How is contrast achieved in light microscopy?

The PYR test uses what reagent to produce a bright red color?

Which of the following is used to emulsify microorganisms from a solid media.

Fluorescent microscopy uses what to fluoresce?

A positive catalase test produces what reaction?

The lysis of red blood cells gives which media its color?

Which of the following describes a principle of bacterial cultivation?

What are the three key components of microscopy?

___________ are used to record the metabolic profile of a bacterium in tests such as the API.

Why is a Standardized inoculum used?

Phase-contrast microscopy is used to identify which types of organisms?

Phase contrast microscopy is used to identify medically important fungi grown in culture.

___________ tests if an organism can grow in the presence of a single nutrient/carbon source

A population of microorganisms cultivated in a medium of nutrients.

Size, pigmentation, shape and odor are all exaples of?

What is the purpose of a 420C incubator?

The objective lens combined with the 100X objective magnifies objects _______X its normal size.

_____________identifies a bacteria's ability to produce the enzyme tryptophanase

Bacteria nutritional needs are usually classified as_____ and ________.

Crystal violet performs what function in a gram stain?

In the Gram stain process, which stain is called the counterstain?

The objective lens combined with the 40X objective magnifies objects _______X its normal size.

All are modes of action of antibacterial agent EXCEPT?

Fluorochrome used to detect fungal elements cells.

With electron microscopy, total magnification can reach _____ normal size.

Carbolfuchsin is the primary stain in which staining method?

Add powder to a beaker of distilled water, place it on a burner to prepare_____________

Specifically designed for a subset of bacteria whose cell walls contain long chain fatty acids.

Light pass through the specimen and are partially deflected by different densities of the cells

A _____________ is used for rapid preliminary organism identification by direct visualization

For staining microorganisms grown in culture, which of the following can be used

Two common ways to affix microorganism to a slide is by______.

When is confluent bacterial growth on a plate used?

_________ is used to enhance the growth of a particular bacterial pathogen

What reactions are recorded from a gram stain?

Which of the following is an application of microscopy in diagnostic microbiology?

Contain a thick peptidoglycan layer with numerous teichoic acid cross linkages.

Fluorochrome used to detect bacteria.

Consist of a thin layer of peptidoglycan.

Basic and straight-forward nutritional needs.

A light microscope performs?

Kinyoun acid fast staining method is also known as the _____ method.

________________utilization is an important factor in bacterial identification.

Liquid media is known as what?

The cultivation and/or testing of microorganisms within laboratory vessels.

The extent to which detail in the magnified object is maintained.

What is the primary stain used for the microscopic examination of bacteria?

The cultivation and/or testing of microorganisms within living organisms.

In the Gram stain process, which stain bonds alkaline dye to the bacteria cell wall?

The initial test used for differentiating between groups of Gram Negative bacteria

Red blood cells and white blood cells stain pink in a smear.

Interruption/disturbance of one or more steps essential for effective antimicrobial action.

How is magnification achieved in light microscopy?

Strict anaerobes will not tolerate___________.

Define genotype.

Campylobacterspecies uses special media for recovery known as?

Classic acid fast staining method is called?

Acid fast bacteria do not gram stain well because they have______________.

In the Gram stain process, which stain is called mordant?

Once a smear is made the microorganisms are allowed to dry and is affixed.

To successfully kill a microorganism, the _______ form of the antimicrobial agent must be used.

How many hours of incubation do most conventional identification schemes require?

Dark-field microscopy is used to identify which organisms?

Resistance resulting from normal genetic, structural, or physiologic state of a microorganism

Dark field microscopy is used to detect Spirochetes.

How are broth dilutions measured when testing organisms with antimicrobial agents?

A simple method of making an alternative CO2 incubator is?

The objective lens combined with the 10X objective magnifies objects _______X its normal size.

Generally specimen samples are placed on the slide using which of the following.

Electron beam scans the surface and provides a three dimensional view.

Uses electrons instead of light to view specimens.

Media containing nutrients used to support the growth of bacteria in the laboratory.

Have very specific, complex growth requirements.

Shigella and salmonella can be recovered on which agar?