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Lab Practical #2 Review Session Flashcards | StudBud
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Lab Practical #2 Review Session
Lab Practical #2 Review Session
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Questions
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Answers
1
Antibiotics do not cause resistance, they
select
for
resistant organisms that already exist.
False
True
2
The
bla
gene in the pGLO plasmid codes for beta lactam synthesis.
False
True
3
Answer the question below:
O
A
AB
B
4
According to the Colilert result shown in this image, we can deduce that
E. coli
is present in the water sample tested.
True
False
5
Which is/are correct regarding disinfectants and antiseptics?
disinfectants are more harsh than antiseptics
antiseptics are used on body surfaces and living tissue
antiseptics are used to disinfect inanimate objects
disinfectants are used to disinfect inanimate objects
6
The MR portion of the MR-VP test...
indicates that an organism uses the mixed-acid fermentation pathway
will turn maroonish-brown if the result is positive
will turn red if the result is positive
indicates the organism uses the 2,3-butanediol fermentation pathway
7
The patient from the previous question would also benefit from a shot of Rhogam.
True
False
8
Coliforms are commonly used indicator organisms in water microbiology. They indicate the presence of _____ in water.
spores
fungi
fecal matter
dirt
9
The only way to be positive for the nitrate test is pink after zinc.
True
False
10
Given the choice, which is a better option for the health and safety of others?
washing hands with soap and water
using hand sanitizer
washing hands with soap and water, then following up with hand sanitizer
none of the above
11
What is the purpose of adding starter culture to sweet tea when making kombucha?
No good booch should go to waste!
The starter provides a boost of bacteria and yeast to get the culture going
The tea will taste too sweet without it, this adds a more tangy flavor
The starter lowers the pH of the tea, decreasing chance of contamination
12
Using the MTFM method, determine how many coliforms/100 mL are in the sample. Table is on pg 148. Sample undiluted.
not shown on this table
21
75
11
13
sanitization is the process of removing
all
living cells, spores, and viroids
True
False
14
Based on the results of this plate, the compound tested is
not
a carcinogen.
True
False
15
This plate of
Micrococcus luteus
was exposed to UV. Area B was covered with foil.
False
True
16
The white blood cell furthest to the left is a _____________.
lymphocyte
monocyte
eosinophil
neutrophil
basophil
17
To which component of the ELISA is a colorimetric enzyme attached?
Secondary antibody
Antigen
Patient sample
Chromagen
Primary antibody
18
In an indirect ELISA test, what are scientists attempting to detect?
antibodies in the patient's serum
antigen in the patient's serum
antigen in the patient's RBCs
anti-human antibodies
19
Why is the water at the WWTP churned as it is passed under UV light?
to ensure a homogenous mixture of grey-water
UV can't penetrate surfaces, so churning increases surface area exposed
to evaporate off the water and concentrate the coliforms
none of the above
20
Real-time PCR is
quantitative
whereas traditional PCR is
qualitative
.
True
False
21
Food ___________ is consumption of the microorganism, followed by its multiplication and invasion
toxicity
poisoning
intoxication
infection
22
What about now?
yes, it's glowing under UV light, so it's E. coli
still can't determine if it's E. coli
No, the yellow color of the media in the previous pic indicates a negative
23
Indole production is definitive for
E. coli
.
True
False
24
Organism B is capable of using citrate as its sole carbon source.
True
False
25
The MacConkey agar plate was streaked with an unknown organism. Could the organism be
E. coli
?
No, only Gram positives can grow on MacConkey and E. coli is Gram negative
Yes, this organism clearly ferments lactose (and so does E. coli!)
No, this organism is a non-fermenter and E. coli ferments lactose.
26
Is the organism inoculated on this spirit blue plate able to secrete lipase?
not enough info, iodine needs to be added to the plate before reading.
yes
no
27
Based on the color of this P/A broth, the water is potable.
False
True
28
Which plate would you expect to have
no growth
?
-pGLO, LB
-pGLO, LB/Amp
+pGLO, LB/Amp
+pGLO, LB/Amp/Ara
29
An organism will never be negative for both MR and VP in the MR-VP test, it has to be positive for one or the other.
False
True
30
Which component in the plasmid codes for the "light switch" to turn the GFP gene on or off?
the gfp gene
the bla gene
the ori
the araC gene
31
Which is matched incorrectly?
Martin's media- mold
TSA- "other bacteria" in soil
Martin's Media- lactic acid bacteria
Jensen's media- Streptomyces
MRS-BPB- lactic acid bacteria
32
This urea test result is...
definitive for Proteus mirabilis
negative
positive
definitive for Klebsiella pneumonaie
33
The theory behind the Ames Test is that exposure to a carcinogen will ___________.
allow Salmonella typhimurium to grow on media that lacks histidine
render Salmonella unable to grow on GSA
mutate the DNA of Salmonella typhimurium
knock out the fxn of the histidine synthesis gene in Salmonella typhimurium
34
The clear zones in the lawn of bacterial growth on this plate are called _________.
zones of inhibition
colonies
lysogenic phages
plaques
35
If the scientist forgot to add antigen to the well prior to adding patient serum, what would occur?
the antigen is IN the patient serum, so nothing is wrong
the enzyme would not convert the substrate properly
a false positive result
a false negative result
36
What is the goal of replica plating?
To look for colonies that are missing on a plate that lacks the amino acid
To identify prototrophs
To make a stamp of a plate w/ the amino acid onto a plate lacking it
To identify auxotrophs
37
SCOBY stands for Similar Cultures of Bacillus and Yersinia
True
False
38
According to the gel below, which sample(s) likely contain
Bacillus subtilis
?
sample 4
sample 3
sample 2
sample 1
39
Which of the following is/are example(s) of an antiseptic?
iodine
lysol
mouthwash
hand sanitizer
bleach
clorox
40
Indirect selection is also known as replica plating.
False
True
41
A person with AB blood has both the A and B antigen on their red blood cells
False
True
42
In the Kirby-Bauer method, the antibiotic with the largest zone of inhibition will always be the most effective one.
True
False
