Chapter 9: Biotechnology and DNA Technology

What are self-replicating DNA molecules used to transport foreign DNA into a cell?

What can be used to observe amplified DNA by separating the products by size and comparing it to other DNA samples?

Which of the following is true of recombinant DNA technology?

RecDNA is possible because DNA is DNA is DNA.

Saccharomyces cerevisiae is easily grown and has a larger genome than bacteria.

Which of the following is not an ethical and safety concern of using DNA technology?

The "sticky end" of DNA, the complementary DNA end, is produced by restriction enzymes on the DNA fragment to be cloned.

Which of the following are agricultural applications of biotechnology.

Which of the following is true about gene therapy?

The insertion or modification of genes to produce a desired protein is called gene therapy.

Which is false of the Human Genome Project?

What is the enzyme that sticks the two sticky ends of DNA fragments together?

PCR of stool samples could be used to confirm Norovirus infection, a common cause of acute gastroenteritis.

Which of the following pairings is correct?

E. Coli expresses eukaryotic genes easily.

Nonpathogenic viruses carrying genes for a pathogen's antigens are used for DNA vaccines.

These are made from pathogen proteins in genetically modified yeasts

E. Coli is used to make insulin.

After transformation, the bacteria will either be grown to produce gene product or DNA isolation will occur.

The analysis of data involving biotechnology and human sequencing has produced a new field called Bioinformatics.

During PCR denaturation, the high temperatures will separate the DNA strand.

What do restriction enzymes do?

What organisms can be used to make gene products?

The Human Proteome Project is the mapping of all human proteins and will revolutionize our understanding of biology.

PCR, polymerase chain reactions, are used for...

Which of the following are advantages to using E. Coli to make a gene product?

What must be added to PCR before cycling begins? (if DNA sample is already in the tube)

What are the steps of PCR in order?

Which happens during PCR annealing step?

Why are the disadvantages to using mammalian cells to make gene products?

Which of the following are examples of products made from biotechnology?

Plant cells express eukaryotic genes easily, are easily grown, and are low-cost, making them good for gene production.

Biotechnology is the use of microbes, cells, or cell components to make a desired product.

What is transformation as it relates to recDNA?

How can DNA be inserted into a cell?