LTA's

Gastrulation is primarily an example of

What is smaller?

I want to solubilize (without denaturing) a membrane protein. I should use

Confocal microscopy: scans the specimen with a focused laser to get 2 dimensional sections

In a gene, introns are kept while exons are removed

Most histone modifications are covalent modifications to synthesized histones. Why is this necessary for chromatin reg?

Ribosomes must bind to the 5’ cap before initiating translation

Which transmembrane protein does not have its N-terminus in the ER lumen?

Does Apaf1 bind bax?

Would putting an antibody that brings two RTKs together cause MEK to be phosphorylated?

Fos and Jun are

What is a good definition for protein domain?

Pex proteins function in the peroxisome

A has 2 hydrogen bonds with T while C has 3 hydrogen bonds with G

How might one determine where ubiquitin is located in the cell

Where is Cdc14 held “inactive”

Which of the following proteins are not used during DNA repair

Which proteins help the correct tRNA occupy the A site?

What type of electron microscopy submerges a sample into an electron dense liquid that shows up around it?

does cGMP cause sodium channels to open in the visual system?

Which of the following phospholipases are correctly paired with one of their products

The SRP binds nascent polypeptides with 15 hydrophobic a.a’s and locks the ribosome onto the translocon of ER membrane

SAMs are places of growth

Would introducing a mutation in Ras cause MEK to be phosphorylated?

What kind of protein adds phosphates to another protein or lipid?

Which of the following is NOT the role of molecular chaperones in the folding of cellular proteins?

The neural tube is ectoderm that will become the nervous system

Passing cells is what you do to start a new culture

siRNA’s can bind to the RITS complex

Spindle assembly checkpoint occurs after mitosis

The process by which the ribosome small subunit starts at the 5’ cal and find the start codon is called

I centrifuge a cell extract gently and take the Supernatant I centrifuge at high speed and take the pellet. I have

A retrograde vesicle that left the cis-golgi would… Choose all correct answers.

Which will have the most genes made inaccessible in heterochromatin?

Collagen genes are highly expressed. They are likely to be found in

During the process of nuclear import, a GEF works in the:

G alpha t is associated with cGMP phosphodiesterase

Which value tells you how well your mean represents the population?

Silencers decrease expression of a gene by packaging it tightly into heterochromatin

Transmembrane proteins

Do oats and other grain plants have cellulose in their cell walls

In a double helix

Is the geometry tetrahedron made by a lipid?

Which of the following are tyrosine kinases?

Which process recycles NADH when there is no oxygen?

What process is responsible for the formation of a thumb?

Oats and other grain plants do photosynthesis

Pectin is used to make honey

Which is not a valid part of protein trafficking?

What is not a storage option for metals in cell biology?

What process uses DNA ligase?

What protein works ahead of replication fork?

Which type of electron microscopy submerges a sample in an electron dense liquid that shows up around it?

FACS: you want to separate cells that are expression protein A

areas of sphingolipids and cholesterol that are more related and ordered than the fluid around membrane are?

What type of ground tissue is found in roots?

Which step in cytoskeleton polymerization is rate limiting?

Ruffle-like membrane protrusions are called?

Okazaki fragments are small fragments of DNA that eventually are located to form which of the following strands of DNA?

Which cellular structure contains unconventional myosin?

Dr. KB is

Where are skin stem cells found

Which vesicular coat is involved in going from the ER to the cis golgi?

PDI has nothing to do with peroxisomes

Which are phosphorylated by JAK’s?

Is Bad detected by Brdu?

PTSI/PTS2 signals sequence targeting proteins to peroxisomes

Which cell junctions are not associated with actin cytoskeleton?

Which of the following is not needed for PCR?

Phloem & xylem surround meristems

siRNA’s are one source of euchromatin

Retroviruses contain RNA

Pulse chase: you would use this to see where your protein was transported to in the cell

Fluorescence microscopy: uses a light microscope with a optical component to take advantage of the different lights

Which of the following factors recognizes the UAG, UAA, and UGA codons?

Is Bad found in the outer mitochondrial membrane?

SiRNA’s cause transcription repression through histone methylation

Why doesn’t Na go through a resting K channel?

Does the retina change shape in response to sound?

Laminin is a

Proteins to be degraded are modified by a single ubiquitin

Immortalized cells will always have contact inhibition

Confocal microscopy: has the ability to resolve cellular component as small as 2nm

Would activating RAF cause MEK to be phosphorylated?

Yeast 2 hybrid: you want to see if protein A interacts with protein B

Which is not a way to reduce out of focus light to fluorescence microscopy?

RNA cleavage involves mitochondria

Cell fractionation: you want to see where your protein localizes in a cell

Which of the following is seen when a single mRNA is simultaneously translated to form multiple copies of a polypeptide

siRNA’s cause transcription repression through histone methylation

In a double strand dna molecules one of the chains has CCCATTCTA. There are no C residues in other chain

Immortal cell lines are cells that can continue to proliferate in culture for an indefinite # of generations

PDI functions in the peroxisome

unreplicated DNA checkpoint happens at G2 M

GLUT proteins are glucose

Will statistical analysis tell us the reason an error occurred in measurements?

G alpha q is associated with adenylate cyclase

Phloem transports sucrose away from where it’s made

Lignin is not waterproof

What type of protein is APC?

What protein is recruited by Grb2 to the plasma membrane?

The TATA box is only used for rRNA transcription

Catalase neutralizes peroxides

Which organelles are correctly paired with the signal sequences that sort proteins to go inside them?

G alpha s is associated with adenylate cyclase

Which of the following is NOT a DNA sequence

The plaque assay takes advantage of the lysogenic viral cell

Spindle assembly checking happens with metaphase and anaphase

If a protein is part of the citric acid cycle, which translocon would be used?

Which type of endocytosis is associated with transferrin

Does bad bind apaf-1?

Which are produced by the dark reactions of photosynthesis?

If the concentration of free monomers in solution is -, the critical concentration will grow

In cell culture, what is a "callus"?

All checkpoints have to be functioning in order for a cell to complete a cell cycle

There are two ways to regulate which genes are transcribed. Which term does not fit with the other 3?

MDR protein pumps drugs out of the cell

Phosphatidyl-inositol: phosphoglycerides

Central dogma

Which statement is NOT correct regarding electron microscopy?

FRAP: you want to see if a fluorescent tagged membrane protein can move

Proteins are synthesized from the N terminus to the C terminus by a ribosome

The ends of chromosomes are called what and are maintained by what enzyme?

What does BrdU stain?

Fluorescence microscopy: required two sets of filters

Palmitate (16:0) is a ____ with ____ carbons and _____ double bonds

All of the following proteins interact with exposed amino acids during protein folding in the ER except

What is not part of chromatin remodeling?

Nonpolar amino acids tend to be found in the interior of proteins

What is another name for a death receptor

FISH: you use a probe to see if there are multiple copies of a gene present

Nucelosapsid is a protein coat with DNA inside

Chromosome segregation checking happens after the exit from mitosis

Which of the following small GTPases are NOT involved in vesicle budding or docking?

Which of the following pairs of codons might you expect to be ready by the same tRNA as a result of wobble

Which statement regarding the marking of a protein by polyubiquitylation to signify degredation is true?

Which proteins are necessary for the establishment of the plasma membranes resting potential?

RNA pol 2 is responsible for transcribing mRNA

Which type of microscopy has the best resolution?

Which is not a place the DNA replication transcription or translation starts?

KB or Karam?

Does differential centrifugation separate organelles based on shape/size/mass?

RNA pol 1 is responsible for transcribing rRNA

Ca 2+ pump moves Ca 2+ into the ER and SR

DNA sequencing does not use DNA ligase

RNA cleavage involves mRNA, tRNA, occurs in prokaryotes and eukaryotes

“The actin was found in bundles extending the length of the cell” is an example of what kind of statement?

Do oats and other grain plants have angiosperms

FRET: you look for expression of a blue color to see if two proteins interact

The neural tube is just about the notochord, a rigid structure that provides it with support

A CDK is active when its phosphorylated on theronine 161 and deposphorylated on tyrosine 15

An envelope is a membrane under the capsid

Which type of endocytosis is associated with transferrin?

Bright field: employs a light microscope and requires that the samples be fixed and stained in order to reveal details

what does the TATA box recruit?

What kind of protein removes phosphates from another protein or lipid?

Myosin light chain binds the neck of myosin heavy chain and is activated by Ca

Which phospholipase would cut phosphatidylcholinr into choline and phosphatic acid?

FISH: you would use this if your protein of interested was a voltage gated channel

Which enzyme adds ubiquitin to the protein to be degraded?

Is Apaf1 a pro caspase?

Phloem is the inner layer of wood

DNA damage checkpoint happens at any point in cell cycle

Cholesterol: phosphoglyerides

Which lipid is not glycerol based?

Pseudogenes

siRNA’s cause translation repression through histone methylation

Collagen is usually found as euchromatin

Cholesterol is neither phosphoglycerides nor spingolipids

Pulse chase: you want to see where your protein localizes in a cell

An enlongating ribosome is bound to appropriate tRNA’s in both the A and P sites. What happens next?

Which statements are true regarding DNA replication?

Name one way to make bright field microscopy better

Scanning electron: required coating sample with a thin layer of a heavy metal

Unlike DNA, RNA uses a uracil base and a deoxyribose sugar

Pectin is used to make jelly

What process is normally used to turn Rb off?

RNA editing…

Histones are not often

Reaction enzymes are endonucleases; if they cut both strands at the same place, blunt ends result

Which of the following is not a DNA binding domain?

G alpha I is associated with adenylate cyclase

Which is not a stop codon?

Patch clamp: you would use this if your protein of interest was a voltage gated channel

Which proteins are in the ER?

In a gene, exons are kept while introns are removed

Which statement is true regarding tRNA’s?

Why would a transverse diffusion or flip flop of membrane phospholipids from one leaflet to the other be so rare?

If a protein has a zinc finger, it is most likely a

SiRNA’s can bind to the RITS complex

What stages of mitosis are correctly matched with what happens

All RNA polymerases transcribe the same genomic code

What makes cultures cells useful models to study human disease?

TAF recruits RNA pol to the TATA box

FACS: you look for expression of a blue color to see if two proteins interact

Plants have vacuoles instead of lysosomes

During the import of proteins into the nucleus the importin binds directly to

Xylem transports fluid down the plant

Phase contrast: uses a light microscope with a optical component to take advantage of light passing through

Which of the following protein kinases are downstream of a G-protein coupled receptor?