Organic growth factors

Are obtained from the environment and include vitamins and amino acids

Which of the following is a function performed by bacteria which would be vital to life on earth?

Decomposing organic material and recycling nutrients

Differential Interference Contrast Microscopes (DIC)

Produce colored, three dimensional images

The purpose of a mordant in the Gram stain is

To prevent the crystal violet from leaving the cells.

May contain dipicolinic acid (calcium dipicolinate)

What happens when a bacterial cell is placed in a solution containing 5% NaCl (hypertonic solution)?

Water will move out of the cell.

Poly Beta hydroxy butyric acid granules (PHB):

Answer 4 of 4 textEdit Answer 4 of 4 textAre used for storage

Plasmids or episomes of bacteria

Contain nonessential bits of information

Grow in oxygen concentrations less than those found in air

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Micro 2230 WEB: Module 1 Quiz practice quiz

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Micro 2230 WEB: Module 1 Quiz quiz

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Answers

1
You have isolated a cell from nose. It has Gram +ve cell wall & no visible nucleus. You can safely assume that the cell
is anaerobic
Is procaryotic
Has mitochondria
is a thermophile
2
The best use of a negative stain is to see the endospore
False
True
3
Aerotolerant aerobes do not use oxygen for growth but are not poisoned by it
True
False
4
Organic growth factors
Are obtained from the environment
Include vitamins and amino acids
Are obtained from the environment and include vitamins and amino acids
Include copper
5
The iris diaphragm of a microscope regulates light allowing for contrast
False
True
6
Fungi are Eucaryotic organisms
True
False
7
Osmosis involves Water, semipermeable membrane, and concentration gradient
False
True
8
The best use of a negative stain is to determine the cell shape
True
False
9
Bacteria use phosphorus to form cell membrane phospholipids
False
True
10
The eyepiece of a microscope in most cases magnifies 10X
False
True
11
Only unicellular organism cause infection
True
False
12
Which of the following is a function performed by bacteria which would be vital to life on earth?
Directly controlling insect populations
Directly providing humans with food
Causing disease
Decomposing organic material and recycling nutrients
13
You observe through a microscope at 1000X a cell that has no apparent nucleus. You conclude that it most likely:
Has a peptidoglycan cell wall
Moves by means of pseudopodia
Has a cellulose cell wall
Is part of a multicellular animal
14
A confocal microscope may be used along with a computer to construct three dimensional images
False
True
15
You stain Bacillus by malachite green with heat & then stainin with safranin Under microscope green structures you see
is cell wall
is Capsule
is Endospores
is lagella
16
Hydrogen peroxide (H2O2) Is broken down by enzyme catalase
True
False
17
You would use a complex medium to Isolate a "selection" of organisms from the environment
True
False
18
The resolution of a microscope can be improved by changing the:
Diaphragm
Wavelength of light
Fine adjustment
Condenser
19
Differential Interference Contrast Microscopes (DIC)
Use differences in wavelength to produce an image
Have a lower resolution that phase contrast microscopes
use fluorescent light to produce an image
Produce colored, three dimensional images
20
Gram's iodine iIs used as a mordant
False
True
21
All bacteria are unicellular
True
False
22
Germ theory was proposed by van Leeuwenhoek
False
True
23
The purpose of a mordant in the Gram stain is
To make the flagella visible.
To prevent the crystal violet from leaving the cells.
To remove the simple stain.
To make the bacterial cells larger.
24
Spores of bacteria
Are used for movement
Are used for storage
May contain dipicolinic acid (calcium dipicolinate)
Contain nonessential bits of information
25
Malachite green is never used in the endospore stain
False
True
26
What happens when a bacterial cell is placed in a solution containing 5% NaCl (hypertonic solution)?
Water will move out of the cell.
Water will move into the cell.
No change will result .
The cell will undergo osmotic lysis.
27
Clostridium are obligate aerobes
False
True
28
An organism with monotrichous flagellar arrangement has a single polar flagellum
True
False
29
Gram Iodine (Iodine) is used as mordant in Gram staining
True
False
30
Important in the early studies involved in spontaneous generation: (Redi's work)
Malachite green
Salvarsan
"Vital force"
Koch's postulates
31
Hansen's bacillus is not a scientific name
False
True
32
Phycology is a branch of microbiology dealing with:
Mycelium
Fungi
Algae
Protozoa
33
Poly Beta hydroxy butyric acid granules (PHB):
Allow movement
Answer 4 of 4 textEdit Answer 4 of 4 textAre used for storage
May contain dipicolinic acid
Allow the bacterium to survive adverse environmental conditions
34
Louis Pasteur disproved the theory of spontaneous generation?
True
False
35
Plasmids or episomes of bacteria
Are used for storage
Contain nonessential bits of information
Are used for movement
May contain didicolinic acid
36
The log phase of growth is the phase during which maximum growth occurs
False
True
37
Thioglycollate medium is used for culture of anaerobes
True
False
38
All Bacteria are Eukaryotic
False
True
39
Thioglycollate medium is used for culture of Psychrophiles
True
False
40
A negative stain is frequently used to stain Gram negative bacteria.
False
True
41
Acid fast stains allow identification of bacteria containing mycolic acid
True
False
42
An organism with amphitrichous flagellar arrangement has a single flagellum at each end of the cell
False
True
43
This microscope is used to see internal structures of cells in a natural state.
Electron microscope
Fluorescence microscope
Phase-contrast microscope
Compound light microscope
44
An organism contained peptidoglycan & dipicolinic acid (calcium dipicolinate).What was the genus of organism
Staphylococcus
Escherichia
Bacillus
Treponema
45
A laboratory designed to study an extremely deadly pathogen would be designated BSL4
True
False
46
Microaerophiles
Are poisoned by free oxygen
Grow in oxygen concentrations less than those found in air
Require free oxygen to live
Do not use oxygen but are not poisoned by it
47
The most probable number represents a statistical estimation of the number of bacteria in a sample
True
False
48
Placing cells in an hypertonic solution would result in shrinking (plasmolysis) of the cells?
False
True
49
Where are phospholipids most likely found in a procaryotic cell?
Flagellae
Around organelles
Plasma membrane
Ribosomes
50
Acid alcohol is used as a decolorizer
False
True
51
A high concentration of sodium chloride (salt) in a medium would allow growth of Psychrophiles
False
True
52
Bacteria use nitrogen to form Amino groups of proteins
True
False
53
Mycology is a branch of microbiology dealing with Fungi
True
False
54
Saprophytes obtain nourishment from dead organic material
False
True
55
41 colonies were found on a plate prepared from a 1/100 dilution. How many organisms were present in the original sample
410
41000
4100
41
56
Scanned probe microscopes uses probes to examine the surface of a specimen without altering it
True
False
57
Immersion oil has the same refractive index as glass and reduces light scattering
False
True
58
A high concentration of sodium chloride (salt) in a medium would allow growth of Halophiles
False
True
59
The genus Bacillus contains organisms with Gram positive rod shaped cells
True
False
60
Bacteria use sulfur to form
Vitamins such as thiamine and biotin
Amino groups of proteins
Carboxly groups of proteins
Cell membrane phospholipids
61
Placing cells in an hypotonic solution would result in __________ of the cells?
lysis
binary fission
Swelling
Shrinking
62
Carbol fuchsin:
Is used as a decolorizer
Is used as a counterstain
Is used in the spore stain
Is used in the acid fast stain
63
Who observed cells in plant material?
Hooke
van Leeuwenhoek
Koch
Pasteur
64
Yeast and Protozoa are not Eukaryotes
True
False
65
You would use a chemically defined medium to grow an organism with exacting growth requirements
False
True
66
A green zone surrounding a colony grown on blood agar would be termed _______ hemolysis.
Beta hemolysis
Answer 1 of 3 textEdit Answer 1 of 3 textAlpha hemolysis
Gamma hemolysis
None of these
67
Inorganic trace elements needed by microbes Include iron, copper molybendenum & zinc & often serve as enzyme cofactors.
False
True
68
The lag phase of growth Is the phase in which the bacterium adjusts to a new environment after transfer
True
False
69
Place the steps of the Gram stain in the correct order: 1-Alcohol-acetone; 2-Crystal violet; 3-Safranin; 4-Iodine.
2-4-1-3
4-3-2-1
1-3-2-4
1-2-3-4
70
Most bacteria possess cell wall as well as plasma membrane
True
False
71
Superoxide free radicals:
Are formed by organisms using free O2 as final electron acceptor making H20
None of these
Are transient reactive molecules formed in cytoplasm by ionizing radiation
Are produced by superoxide dismutase
72
Axial filaments function in:
Resistance to phagocytosis
Energy matabolism
finding a mate
Movement
73
Scanning Acoustic Microscopes:
Uses probes to examine the surface of a specimen without altering it
Could be used to study biofilms
produces colored flat images
Uses fluorochromes and a laser
74
Obligate aerobes require free oxygen to live
False
True
75
What is the total magnification of a chloroplast viewed with a 10x ocular lens and a 45x objective lens?
450x
45x
100x
10x
76
Where would you most likely find a psychrophile?
In blood
in the ocean
in sick patient
A hor Spring
77
You would use a differential medium to Destinguish between Escherichia coli and Enterobacter aerogenes
True
False
78
A green zone surrounding a colony grown on blood agar would be termed Alpha hemolysis.
False
True
79
Jenner developed vaccination
False
True
80
A change in pH in a culture medium often slows microbial growth. This pH change is often due to:
Failure of organisms to excrete large amounts of pH
Accumulation of waste products
Lack of vitamins
Answer 4 of 4 textEdit Answer 4 of 4 textInadequate sanitation facilities
81
West Nile encephalitis is spread to humans by mosquitoes
False
True
82
Hydroxyl radicals are a transient highly reactive substance formed in cytoplasm by ionizing radiation
True
False
83
Autotrophs derive energy from Carbon dioxide
False
True
84
Singlet oxygen is an extremely reactive high energy form of oxygen
False
True
85
Chemoheterotrophs derive energy from Carbohydrates and other organic materials
True
False
86
Which of the following is a scientific name?
Shingles
Group A streptococcus
Streptococcus pyogenes
Streptococcus pyogenes and Strep
87
Streak plate method is a technique which relies on the fact that one cell theoretically forms one colony
True
False
88
Luis Pasteur is famous for introducing "Aseptic Technique"
True
False
89
The Scientist Ehrlich is related with Salvarsan
True
False
90
Mycobacterium leprae is not a scientific name
True
False
91
The most probable number gives a statistical estimation of the number of organisms in a sample.
False
True
92
Leprosy bacterium is a scientific name
False
True
93
The condenser of a microscope focuses light on the specimen
False
True
94
You would use a selective medium to Isolate Staphylococci from salami
True
False
95
Obligate anaerobes are poisoned by free oxygen
False
True
96
You would use a candle jar to isolate a capnophile
False
True
97
Koch invented first microscope
True
False
98
Nigrosin is used in the negative stain
False
True
99
Alexander Fleming Discovered penicillin
True
False
100
Paul Erlich used salvarsan to treat syphilis
False
True

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