How can you determine if something was heated to the proper temperature during sterilization?

Check the autoclave tape for temperature change.

All of the following symbols in some way represent:

The substance is a health hazard.

When streaking a plate, what would be a reason there would be no growth the following day?

All of the choices could be correct

A biosafety cabinent is on the left.

What should you do if you get NaHCO3 in your eyes?

Remove contacts, flush eyes with water for 15 min.

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BACE Review- 5 (Tool, Signs and Equipment)

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1
What is the chemical formula for the product listed?
NaHCO3
Na2CO4
NO2C3
It cannot be determined from this data sheet.
2
What does the symbol below represent?
Do not drink the water
Eye wash station
Safety Shower Location
Toxic or poisonous materials
3
which of the following is not used in the Gram staining
All of these are used
Gram iodine
Crystal violet
Counter stain
4
Used to see if a liquid is acidic or a basic
SOP
Spectrophometer
Balance
pH meter or litmus paper
5
How can you determine if something was heated to the proper temperature during sterilization?
Make sure it was in the autoclave for the required time.
Take the temperature of the substance after autoclaving.
Feel it with your hand to see if it is still hot hours after.
Check the autoclave tape for temperature change.
6
All of the following symbols in some way represent:
Something is on fire or will be soon.
The substance is an environmental hazard.
The substance is a health hazard.
This substance should never be used for anything.
7
What does the symbol below represent?
Do not enter
Harmful or irritant
Biohazard
No eating or drinking
8
Highly sensitive tool used to measure mass using a chamber to prevent the samples from being affected by air currents.
Spectrophotometer
Analytical Balance
PCR Machine
Balance
9
What is the pictured piece of lab equipment?
A hotplate stirrer
A vortex mixer
A plate reader
A balance or scale
10
Name the pictured lab tool.
Masking tape
Autoclave tape
Labeling tape
Petri dish tape
11
What is the 3rd step in the gram staining process?
Add iodine
Decolorize with alcohol
Rinse with water
Add safranin
12
What is the temperature reading on the thermometer below?
65°
90°
95°
85°
13
What is the shape of the red bacteria?
spirillus (spiral)
coccus (sphere)
bacillus (rod)
14
Directions on how to perform a procedure can be found in using the a(n)
Spectrophotmeter
Lab notebook
pH meter or litmus paper
SOP
15
What does this symbol represent?
Biohazard
Radioactive
Harmful or irritant
Radiation hazard
16
What is the purpose of Gram staining?
includes all of these
is used to classify bacteria
detects peptidoglycan in the cell walls of bacteria
makes bacteria visible under microscope
17
Name the pictured piece of lab equipment.
Macro pipette
Micropipette
Transfer pipette
Dropper pipette
18
Name the pictured piece of lab equipment.
Test tube holder
Spatula or scoopula
Foreceps
Test tube rack
19
Name the pictured piece of lab equipment.
Shaking water bath
Ice machine
Microcentrifuge
Thermocycler
20
Identify the meaning of the attached symbol
Fire Extinguisher Location
Defibrillator or AED Location
Hot surface
Toxic Waste
21
What is the shape of the blue bacteria?
Coccus (sphere)
Spirillus (spiral)
Bacillus (rod)
22
During gram staining, what do you use after Iodine?
Alcohol
Safrarin
Crystal Violet
Iodine
23
When placing plates streaked with E.coli, what is the proper way to place them in the incubator
Top side up (lid side up)
Upside down (bottom up)
24
Name the pictured piece of lab equipment.
Crucible
Centrifuge
Macro pipette
Graduated Cylinder
25
What does this symbol represent?
Corrosive
Radiation hazard
Flammable material
Biohazard
26
Used to mix small volume solutions
PCR machine
pH meter or litmus paper
Vortex mixer
Balance
27
When completing a serial dilution, which would be the LEAST concentrated sample?
The stock solution used to start the dillution
The last tube in the series
The first tube in the series after the stock solution
It is impossible to tell without seeing it
28
What does the symbol below represent?
Safety Shower Location
Toxic or poisonous materials
Eye wash station
Do not drink the water
29
The lab equipment pictured is
a graduated cylinder
an Erlenmeyer flask
a micropipette
a microcentrifuge tube
30
Used to maintain records of experiments
Agarose or acrylamide
PCR Machine
Lab Notebook
Centrifuge
31
Main ingredient of a DNA gel and a protein gel?
acrylamide; agarose
Centrifuge
PCR machine
agarose; acrylamide
32
When streaking a plate, what would be a reason there would be no growth the following day?
Bacteria colony was dead/not concentrated enough
Incubator was at the wrong temperature (too hot)
The wrong growing media was used
All of the choices could be correct
33
The lab equipment pictured is
agar
agarose
an incubator
a petri dish
34
What does this symbol represent?
No eating
Food Allowed
No Eating or Drinking
35
Name the pictured piece of lab equipment.
Foreceps
Test tube holder
Graduated Cylinder
Crucible tongs
36
What is the pictured piece of lab equipment?
A hotplate
A balance or scale
A hotplate stirrer
A vortex mixer
37
Which will change pH faster when an acid is added?
Water
Buffer
38
What is the name of the chemical this (M)SDS sheet is for?
Sodium Bicarbonate
It cannot be determined from this data sheet.
Sodium Chloride
Sodium Hydroxide
39
What does the symbol below represent?
Oxidizer
Toxic or poisonous materials
Eye wash station
Hot surface
40
Identify the meaning of the attached symbol
Eye Protection Required
Fire Extinguisher Location
Emergency Exit
Toxic Waste
41
These bacteria are
Gram-positive
Gram-negative
42
The lab equipment pictured is
a micropipette
a graduated cylinder
a beaker
an Erlenmeyer flask
43
What does this symbol represent?
Needs assistance
Hospital
Help
First Aid
44
What would you refer to when disposing of a chemical in the lab?
The SDS
The internet (on a reputable site)
All of the choices are correct
The bottle
45
Which is Gram Negative
Left: Gram Negative
Right: Gram Negative
46
Which is correct?
A fume hood is on the left.
A biosafety cabinent is on the left.
A biosafety cabinet is on the right.
These both serve the same purpose.
47
In the following picture, which is the most concentrated test tube in the protein assay?
Tube 1
Tube 3
Tube 5
Tube 2
48
What does the symbol below represent?
Flammable
High voltage
Radiation
Electrical
49
Name the pictured piece of lab equipment.
Ice machine
Autoclave
Shaking water bath
Thermocycler
50
What is the appropriate temperature for bacteria growth for an incubator?
60° C
23° C
37° C
92° C
51
What should you do if you get NaHCO₃ in your eyes?
Remove contacts, flush eyes with water for 15 min.
Wash eyes out with soap and water
Go out into the fresh air or apply oxygen to the eyes
Information not available.
52
What does the symbol below represent?
Do not drink the water
Safety Shower Location
Eye wash station
Toxic or poisonous materials
53
These bacteria are
Gram-positive
Gram-negative
54
Type of bacteria.
Bacilli
Basophil
Yeast
Cocci
55
What does this symbol mean?
Eye protection
Goggles required
Eye wash station
Splash hazard
56
What shapes are not bacteria cells?
Helixium
Spirillum
Bacillus
Coccus
57
Separates substances based on density
Agarose or acrylamide
Centrifuge
Autoclave
PCR Machine
58
The lab equipment pictured is
a micropipette
a microcentrifuge tube
a graduated cylinder
a macropipette
59
What biomolecule does the Bradford assay test for?
Antibodies
Protein
DNA
Sugar
60
Name the pictured piece of lab equipment.
Carboy
Crucible
Mortor and pestle
Evaporating dish
61
Name the pictured piece of lab equipment.
Crucible tongs
Foreceps
Graduated Cylinder
Test tube holder
62
Gram staining will stain purple if
bacteria have no cell wall
bacteria have a thin cell wall made of peptidoglycan
bacteria two thick cell wall made of peptidoglycan
bacteria have a thick cell wall made of peptidoglycan
63
What does the symbol below represent?
Face shield required
Eye wash station
Goggles required
High UV rays
64
The lab equipment pictured is
a micropipette
a graduated cylinder
an Erlenmeyer flask
a beaker
65
Gram staining is a way to identify bacteria
False
True
66
Is the substance flammable according to the SDS sheet?
Yes
No
67
Type of bacteria
Sperm
Yeast
Rods
Cocci
68
Identify the meaning of the attached symbol
Protective Clothing Required
Biohazardous Waste
Eye Protection Required
None of the choices are correct
69
The lab equipment pictured is
a microcentrifuge tube
a micropipette
a graduated cylinder
a transfer pipette
70
What does the symbol below represent?
Oxidizer
First Aid Station
Toxic or poisonous materials
Hot surface
71
Used to measure absorbance of light (concentration of a sample)
SOP
Centrifuge
Spectrophotometer
Lab notebook
72
What is the measurement shown on this graduated cylinder?
42mL
34mL
40mL
38mL
73
The three shapes of bacteria are...
rod, square, chair
rod, sphere, spiral
rod, circle, chair
74
Name the pictured piece of lab equipment.
Spatula or scoopula
Erlenmeyer Flask
Carboy
Bunsen burner
75
In gel electrophoresis, DNA's charge is _________ and it runs toward the ________ side.
positive, negative
negative; postivie
neutral/negative
negative/neutral
76
When measuring a specific amount of a substance, which pipette would you use?
Whichever one is the closest - even if you have to split the amount.
The smallest that includes the amount needed.
The one that goes to the highest amount.
The smallest one available.
77
Name the pictured piece of lab equipment.
Thermocycler
Ice machine
Microcentrifuge
PCR Machine
78
What does the symbol below represent?
Gloves required
Harmful or irritant
Goggles required
Hot surface
79
When trying to determine the density of a substance, what values do you need to calculate it?
volume and density
mass and weight
mass and volume
volume and height
80
Name the pictured piece of lab equipment.
Transfer pipettes
Pipette pumps
Serological pipettes/pipettes
Micropipettes
81
When completing a serial dilution, which would be the MOST concentrated sample?
The last tube in the series
The stock solution used to start the dillution
It is impossible to tell without seeing it
The first tube in the series after the stock solution
82
Name the pictured piece of lab equipment.
Crucible
Evaporating dish
Carboy
Mortor and pestle
83
What does this symbol represent?
Wash your hands
Radiation
Corrosive
Harmful or irritant
84
How would you determine if you need to wear gloves with a particular chemical?
Take a guess and hope you are right.
Look it up on the SDS worksheet
Look in your lab notebook.
Don't wear them and make sure to wash your hands afterwards.
85
Name the pictured piece of lab equipment.
Erlenmeyer Flask
Carboy
Graduated cylinder
Media Bottle
86
The lab equipment pictured is
a pcr machine
a vortex mixer
a microcentrifuge tube
an electrophoresis chamber
87
When completing a serial dillution what would the concentration be in the final tube?
1x101
1x105
1x104
1x10
88
Used to measure mass
pH meter or litmus paper
spectrophotometer
SOP
Balance
89
When measuring the weight of a substance, which of the following tools would you not use in the process?
scale
weighing boat
scoop/spatula
graduated cylinder
90
The lab equipment pictured is
a mini microcentrifuge
a PCR machine
a vortex mixer
an electrophoresis chamber
91
When weighing a powder chemical, can you put any excess you scooped out back into the bottle?
Absolutely not!
It depends on the chemical.
Yes.
As long as you didn't contaminate it.
92
Name the pictured piece of lab equipment.
Dropper pipettes
Transfer pipettes
Serological pipettes/pipettes
Micropipettes
93
Which of the following amplifies DNA by heating and cooling?
PCR machine
Vortex mixer
Balance
Autoclave
94
Used to sterilize solutions, trash, supplies often with steam, heat & pressure
Autoclave
PCR machine
SOP
Vortex mixer
95
Name the pictured piece of lab equipment.
Graduated cylinders
Erlenmeyer Flasks
Media Bottles
Carboys
96
Used to measure the concentration of hydrogen ions in a sample.
Spectrophotometer
Electrophoresis
Autoclave
pH meter
97
What is missing from the following graph?
All of the choices are missing and should be on the graph.
The x axis is not labeled
Units are not given for the x axis
The main title.
98
What does the symbol below represent?
Fire blanket
High voltage
Flammable material
Corrosive
99
What is the shape of the green bacteria?
bacillus (rod)
spirillus (spiral)
coccus (sphere)
100
Name the pictured piece of lab equipment.
Autoclave
Standard incubator
Shaking incubator
Plate reader

BACE Review- 5 (Tool, Signs and Equipment) Flashcards | StudBud