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Manipulating genomes Flashcards | StudBud
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Manipulating genomes
Manipulating genomes
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OCR A level biology manipulating genomes
Flashcards
33 cards
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Practice quiz
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Questions
33
Answers
1
A plasmid that contains newly added gene is said to contain
recombinant DNA
regulated DNA
transgenic DNA
recognition DNA
2
Which is not true about DNA probes?
DNA probes are short single stranded sections of DNA
DNA probes are double stranded
DNA probes have a radioactive or fluorescent label
DNA probes are used to highlight specific base sequences
3
Which is the correct list of ingredients for PCR?
DNA ligase, primers, nucleotides,
DNA ligase, RNA polymerase, nucleotides
DNA ligase, primers, nucleotides, Taq polymerase,
Taq polymerase, primers, nucleotides,
4
All the genetic material an organism contains is called....
genotype
genome
gene
phenotype
5
What can we use to cut DNA into small fragments?
DNA polymerase
RNA polymerase
scalpel
Restriction endonucleases
6
The most important application of gene therapy is:
treating genetic disorders.
enhancing physical characteristics of human beings.
inserting extra genes into a host cell.
mutating DNA sequences.
7
What is the name given to the modified versions of the nucleotides used in DNA sequencing
terminator nucleotide
terminal nucleotide
final nucleotide
restriction nucleotide
8
What is the original DNA strand? Sanger Sequencing was used.
TAAGCGTTAGT
AUUCGCAAUGA
ATTCGCAATCA
TGATTGCGAAT
9
Which term describes the development of software and computing tools to analyse and store biological data?
computational biology
bioinformatics
10
cDNA production involves which enzyme?
RNA polymerase
reverse transcriptase
restriction endonuclease
DNA ligase
11
What is cDNA?
complementary DNA made from an mRNA template
copied DNA made from a tRNA template
complimentary DNA made from template DNA
RNA made from a DNA template
12
To which end of the electrophoresis gel does DNA move?
positive
negative
13
Which one of these is NOT used as a gene marker?
Antibiotic resistance
Plasmid flag
GFP
Enzymes
14
what do we use to make cDNA from mRNA?
reverse endonuclease
reverse polymerase
reverse protease
reverse transcriptase
15
DNA fingerprinting was developed by
Francis Crick
Henry Locard
Alec Jeffreys
James Watson
16
In the following DNA profile who is the father of the child (Ch)?
1
None
3
2
17
What is the Coding strand? Sanger Sequencing was used.
TGATTGCGAAT
AUUCGCAAUGA
TAAGCGTTAGT
ATTCGCAATCA
18
What is DNA sequencing?
a method for determining nucleotide sequence
the arrangement of DNA in a sequence
a sequence of DNA
the process of altering DNA
19
Which technique may be used to identify individuals or familial relationships?
PCR
DNA profiling
DNA fingerprinting
cloning
20
What do we use to remove/ isolate the desired gene in genetic engineering?
Restriction endonuclease
DNA polymerase
DNA ligase
DNA endonuclease
21
There are short sequences of DNA that is repeated many times (VNTRs), this is called...
virgin DNA
satellite DNA
terrestrial DNA
sky DNA
22
Before the southern blotting process the gel is immersed into alkali to...
break the sugar-phosphate backbone
dissolve the gel
break hydrogen bonds between strands
23
The reason that DNA flows through a gel electrophorisis when electricity is added is because
DNA is positively charged
DNA has no charge
DNA is negatively charged
24
What is Gene Therapy
process of ascertaining the sequence of nucleotides in DNA
the plantation of genes into cells to replce defective ones
25
The correct temperature sequence in a PCR cycle is...
55', 72', 95'
72', 55', 95'
95', 55', 72'
95', 72', 55'
26
What do bands on a DNA profile represent?
Chromosomes
Chromatids
DNA fragments
Genes
27
Gel Electorphoresis is used to:
Identify suspects
Transplant genes into plant cells
Identify paternity
Make copies of DNA
28
What information can genetic testing give?
Show whether a person is a carrier of a genetic disease
Display missing proteins that can lead to disease
Determine when a person will develop a certain disease
29
What does PCR stand for?
Polymerase chain reaction
Polymer channel reaction
Polymerase channel reaction
Polymer chain reaction
30
Non-coding regions in DNA are called...
introns
exons
31
PCR is used to...
Catch criminals by separating DNA strands
Create new genes
Amplify a DNA sample
Break DNA up into single bases
32
Would techniques could be used to profile crime scene DNA?
Gel Electrophoresis and Paternity Testing
Test Crossing and Pedigree Analysis
PCR and Gel Electrophoresis
Paternity Testing and PCR
33
Which process is a screening process to check embryos don't have an unwanted genetic disorder?
GIFT
IVF
PGD
ICSI
