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Cell fractionation and ultracentrifugation Flashcards | StudBud

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Cell fractionation and ultracentrifugation

11 terms·StudBud Administration·44 plays

A biology quiz to make sure lower sixth students understand the basics of cell fractionation.

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Questions 11

Answers

1
what is the purpose of cell fractionation?
to break down tissues
to break down cells
to study specific organelles
because biologists have nothing better to do
2
why is a cold solution required
so you don't burn yourself
to prevent osmosis
so the pH stays the same
to reduce enzyme activity
3
what is the fluid on top on the sediment called?
filtrate
homogenate
supernatant
isotonic
4
why is an isotonic solution required
so the cell shrinks
to separate the organelles properly
to prevent organelles bursting
so the pH stays the same
5
what is the independent variable of differential centrifugation?
the speed at which the organelles are spun
the type of cell used
the temperature at which the organelles are spun
the amount of time organelles are spun for
6
Which organelle is found in the first pellet?
chloroplasts
mitochondria
golgi body
nuclei
7
To separate the mitochondria you need to spin the supernatent at a slower speed than before
True
False
8
The cells are broken up using a homogeniser (blender)
True
False
9
Why is the homogenate filtered?
To separate the organelles
To maintain the pH
To remove unbroken cells
To remove the nuclei
10
what does the buffer solution do?
prevents cells from denaturing
stops osmosis
prevent organelles from bursting
maintains the pH
11
A centrifuge separates mixtures based on their
colour
charge
magnetism
density