Clinical Chemistry 1 Lecture

MECHANICAL OR AUTOMATIC PIPETS consist of : Air displacement pipet,Positive displacement pipet and Bacteriologic pipet

TYPE II REAGENT WATER-is acceptable for preparation of reagents and quality control materials

Manufacturing chemicals or solutions that do not require higher grades of purity

Air displacement pipet - It relies on piston for suction creation to draw the sample into a disposable tip.

It is prepared by using deionizer (anion or cation) and it is free from mineral salts; removed by ion exchangedprocesses

Absence of etched rings; liquid is allowed to drain by gravity

Dispenser/dilutor pipet - It obtains liquid from a common reservoir and dispensed it repeatedly.

ALL OF THE FOLLOWING IS TRANSFER PIPET EXCEPT

Purity is usually delivered by measurement of melting point or boiling point.

International Union of Pure and Applied Chemistry (IUPAC) has established the criteria for primary standard

Type I reagent water-Used for test methods requiring minimum interferences; used immediately after production

Filtration is the first step before processes are performed in reagent grade water preparation.

It delivers exact amount it holds into a container

is the science that deals with the elements, their compounds and the chemical structure and interactions of matter.

w/ graduations to the tip; blowout

Processes involved in the preparation of reagent grade water: distillation, ion exchange, Boiling,Stiring

All of this are types of pipet except

Clinical Laboratory Standards Institute (CLSI)and College of American Pathologist (CAP)have given three grades of water

Class A pipets do not require recalibration

Ultrapure is use in:Chromatography, AAS, Immunoassays

for non-viscous fluid; self-draining

UNIT - Defines the physical quantity/dimension of the result

Is a highly purified chemical that can be measured directly to produce a substance of exact knownconcentration.

Air displacement pipet-It operates by moving the piston in the pipet tip or barrel, much like a hydermic syring.

w/o graduations to the tip; calibrated between 2 marks; self- draining

It is approved for human consumptionbut may not be applicable for laboratory analysis

Is the condensate collected from steam, and created when water is boiled and vaporized

Reagent grade or analytical -Highly purified and has passed thee specifications of purity

Plastic pipet tips are madeprimarily of polypropylene

Comes from the greek word “kline”,

Tests for water purity: microbiological content, pH, resistivity, chemical oxygen demand (COD), ammonia, ions and metals

Is a substance of lower purity whose concentration is determined by comparison with a primary standard

This type of glassware is good for photosensitive analytes

It has a continuous etched rings on top of the pipet; exact volume is obtained when the last drop is blown out

transfer fluid w/o consideration of a specific volume

0.1% phenol red solution in distilled water used to compare the reproducibility of brands of pipet tips

Purity of chemical has been assured by National Bureau of Standards (NBS)

It holds the particular volume but does not dispense the exact volume

Taring refers to the

for viscous fluid; with etched ring

▪ Gravimetric and spectrometric methods - used to verify pipetted volume accuracy and precision

Certified by the College of American Pathologist (CAP)

Reagent grade or analytical - Highly purified and has passed thee specifications of purity

Ultrapure - These type of reagents have been put through additional purification steps

TYPE II REAGENT WATER-For washing glasswares

Distilled water is the calibrating medium for TD pipettes while mercury is for TC pipettes.

PIPET CLASSIFICATION CONSIST OF TWO:

Pipet should be held in a horizontal position with thetips against the side of thereceptacle when in use