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Lab Exam 4 (Labs 15, 17-18, 20) Flashcards | StudBud
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Lab Exam 4 (Labs 15, 17-18, 20)
Lab Exam 4 (Labs 15, 17-18, 20)
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Practice for Lab Exam 4 - summer 2020
Flashcards
44 cards
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Practice quiz
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Host live
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Questions
44
Answers
1
Will
E. coli
WITH pGLO be able to grow on LB/Amp plates?
No
Yes
2
Which of the circles is a positive result for coagulase (in Staphylococcus aureus)?
6
1
3
The "E" in ELISA represents a key component of the test, the ____________.
Every
Ease
Essential
Enzyme
4
Which of the following is a DNA gyrase inhibitor?
Ciprofloxacin
Penicillin
Tetracycline
Azithromycin
5
Does this picture represent a direct or indirect immunological test?
Indirect
Direct
6
Which of the following is a 30s Protein Synthesis inhibitor?
Tetracycline
Azithromycin
Penicillin
Ciprofloxacin
7
Method of controlling microbes used on body surfaces that kills all but endospores.
Disinfection
Antisepsis
Sterilization
Microbial death
8
Which type of immunological test is shown in the photo?
Direct ELISA
Passive Agglutination
Hemagglutination
Indirect ELISA
9
Which of the following chemical agents of control is a disinfectant?
3% Hydrogen Peroxide
Mercurochrome
70% Isopropanol
Bleach
10
What is this test used to identify?
HIV
Group B Strep
Group A Strep
Staphylococcus aureus
11
The LB/amp (DNA-) and LB/amp (DNA+) plates are used to
determine if the bacteria is able to glow.
determine if the original bacteria is sensitive to amp.
determine if the bacteria is viable.
determine if the transformed bacteria is sensitive to amp.
12
Method of controlling microbes that causes all microbes, including spores to be killed.
Antisepsis
Sterilization
Disinfection
Microbial death
13
Which of the following is a 50s Protein Synthesis inhibitor?
Azithromycin
Tetracycline
Ciprofloxacin
Penicillin
14
How did we get our pGLO plasmid
INTO
our
E. coli
bacteria?
Conjugation
Transformation
Transduction
Conformation
15
What bacteria did we use in the pGLO lab?
Diplococcus
E.coli
Salmonella
Streptococcus
16
Which of the following is NOT a condition that must be standardized in the Kirby Bauer test?
Concentration of Bacteria
Temperature and time
Concentration of Agent
Light
17
In an indirect ELISA, what is put into the well second?
Known antigen
Known antibody
Primary Antibody from Patient serum
Secondary Enzyme Linked Antibody
18
In an indirect ELISA, what is put into the well first?
Secondary Enzyme Linked Antibody
Primary Antibody from Patient serum
Known antibody
Known antigen
19
The role of the arabinose in the pGlo lab was to:
kill E. coli
turn on the gene for GFP
provide antibiotic resistance
turn off the gene for GFP
20
The LB/amp (DNA+) and LB/amp/ara (DNA+) plates are used to _________________________.
determine if the bacteria is viable.
determine if the original bacteria is sensitive to amp
determine if the transformed bacteria is sensitive to amp
determine the effect of ara on the transformed bacteria
21
Does this picture represent a direct or indirect immunological test?
Indirect
Direct
22
What type of bacteria grow on the LB/amp DNA + Plate?
bacteria which are resistant to amp
bacteria which are sensitive to amp
bacteria which are sensitive to ara
bacteria which are resistant to ara
23
Which type of immunological test is shown in the photo?
Indirect ELISA
Passive Agglutination
Hemagglutination
Direct ELISA
24
In an indirect ELISA, what is put into the well third?
Substrate
Known antigen
Secondary Enzyme Linked Antibody
Primary Antibody from Patient serum
25
PGLO is
all of the answers
a plasmid
has a gene for GFP
is DNA
26
The role of the arabinose in the pGlo lab was to:
turn off the gene for GFP
provide antibiotic resistance
kill E. coli
turn on the gene for GFP
27
What was the role of ampicillin the pGlo lab?
to kill E. coli without the plasmid
to turn on the gene for GFP
to turn off the gene for GFP
to glow green
28
Which of the following is a halogen?
70% Isopropanol
Bleach
Lysol multipurpose cleaner
100% Ethanol
29
The LB (DNA-) and LB/amp (DNA-) plates are used to
determine if the original bacteria is sensitive to amp.
determine if the bacteria is viable.
determine if the bacteria is able to glow.
determine if the original bacteria is sensitive to ara.
30
In an indirect ELISA, what is put into the well fourth?
Known antigen
Substrate
Secondary Enzyme Linked Antibody
Primary Antibody from Patient serum
31
Which of the following chemical agents of control is an antiseptic?
100% Ethanol
Bleach
Lysol multipurpose cleaner
70% Isopropanol
32
The LB (DNA-) and LB/amp (DNA-) plates are used to _________________________.
determine if the bacteria is viable.
determine if the original bacteria is sensitive to ara
determine if the original bacteria is sensitive to amp
determine if the bacteria is able to glow
33
What type of bacteria grow on the LB/amp DNA + Plate?
bacteria which are sensitive to ara
bacteria which are resistant to ara
Bacteria which are resistant to amp
bacteria which are sensitive to amp
34
Which of the following is a Cell Wall inhibitor?
Tetracycline
Penicillin
Ciprofloxacin
Azithromycin
35
Will
E. coli
WITH pGLO be able to grow on LB/Amp/Ara plates?
No
Yes
36
Which of the following is a surfactant?
Bleach
Lysol multipurpose cleaner
100% Ethanol
70% Isopropanol
37
The LB/amp (DNA+) and LB/amp/ara (DNA+) plates are used to _________________________.
determine if the transformed bacteria is sensitive to amp.
determine if the bacteria is viable.
determine the effects of arabinose on transformed bacteria.
determine if the original bacteria is sensitive to amp.
38
If E. coli produced a 20 mm Zone of inhibition around Penicillin, the bacteria is _________.
Resistant
Intermediate
Sensitive
39
Method of controlling microbes used on inanimate surfaces that kills all but endospores.
Sterilization
Disinfection
Microbial death
Antisepsis
40
Which type of immunological test is shown in the photo?
Direct ELISA
Hemagglutination
Indirect ELISA
Passive Agglutination
41
The LB/amp (DNA-) and LB/amp (DNA+) plates are used to _________________________.
determine the effect of ara on the transformed bacteria
determine if the transformed bacteria is sensitive to amp
determine if the bacteria is viable.
determine if the original bacteria is sensitive to amp
42
Will
E. coli
WITH pGLO be able to grow on LB plates?
Yes
No
43
If Staphylococcus produced a 20 mm Zone of inhibition around Penicillin, the bacteria is _____.
Resistant
Intermediate
Sensitive
44
What is this test used to identify?
Group B Strep
Group A Strep
Staphylococcus aureus
HIV
