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Microbiology
Microbiology
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1
An organism with growth that is enhanced in the presence of low oxygen tension is described as:
Obligate aerobe
Microaerophilic
Capnophilic
Facultative anaerobes
2
The technique that is designed to isolate pure cultures of bacteria is the:
Stab streak
Slant S streak
Candle jar method
Quadrant streak method
3
Tubes are used to hold media that are
liquid
semisolid.
neither semisolid nor liquid
both semisolid and liquid.
4
MAC is used to differentiate Staphyloccoccus aureus from others with this specific change
Red Color Change
Yellow Color Change
Blue Color Change
Green Color Change
5
Culture and sensitivity tests are evaluated by measuring the:
Size of the colonies produced
Number of colonies produced
Concentration of antimicrobials that diffuse
Zone of inhibition
6
Which type of media allow bactera to differentiate into groups by biochemical reactions?
Differentiate
Selective
7
The culture media that is most often used for the growth of fungi and yeasts is:
McFarland
New methylene blue
TSI
Sabouraud agar
8
The primary stain component for most Gram staining kits is:
Fuchsin
Iodine
Crystal violet
Safranin
9
What does MAC Stand for?
MaCaronkey
Maclaroy
Macoy
Maconkey
10
Fluorescent stains are primarily used for the identification of:
Anaerobic bacteria
Mycobacterium
Pseudomonas
Viruses
11
Agar slant tubes are usually inoculated with the:
Slant S streak
Quadrant streak method
Butt stab streak
Candle jar method
12
Inoculating the culture and sensitivity test plate with colonies from a pure culture is referred to as the ___ method
Bauer-Kirby
Standardized disk susceptibility
Indirect sensitivity
Direct sensitivity
13
The counterstain component for most Gram staining kits is:
Crystal violet
Fuchsin
Iodine
Safranin
14
The culture media that is most often used for the growth of fungi and yeasts is:
Sabouraud agar
McFarland
New methylene blue
TSI
15
The purpose of the candle jar is to:
Create anaerobic conditions
Increase carbon dioxide levels
Monitor humidity
Sterilize the jar
16
The mordant component for most Gram staining kits is:
Fuchsin
Iodine
Safranin
Crystal violet
17
Rod-shaped bacteria are described as:
Palisades
Bacilli
Pleomorphic
Cocci
18
The most common cause of diagnostic failure with microbiologic specimens is:
Specimen contamination during collection
A delay in getting samples to the laboratory
Concurrent antibiotic administration
An insufficient sample
19
Type of culture media that contains additives that allow certain microorganisms to grow while inhibiting the growth of
Enriched
Selective
Fastidious
Differential
20
An example of an area that is likely to contain a single organism is:
Fecal samples
Ears
Fistulous tracts
Urine collected by cystocentesis
21
The stain that is primarily used to detect Mycobacterium and Nocardia species is:
Ziehl-Neelsen
Gram stain
Giemsa
Carbol fuchsin
22
Terms that describe the form of a colony on a plate include:
Convex
Alpha
Mucoid
Rhizoid
23
MacConkey agar is an example of
a differential and selective medium.
a general nutritive medium.
a fungal medium.
a differential medium.
24
When Gram stain results are ambiguous, the __________ procedure is performed.
DMSO
Acid-fast
Ziehl-Neelsen
KOH
25
What is the commonly used to grow bacteria in a lab?
Cell Cultures
Soup
Culture Media
Gelatin
26
The most important laboratory procedure for microbiologic diagnosis is:
inoculation of blood agar.
serologic testing.
antimicrobial susceptibility testing.
direct microscopic examination of the specimen
27
An organism with growth that requires high levels of carbon dioxide is described as:
Obligate aerobe
Capnophilic
Facultative anaerobes
Microaerophilic
28
Pathogenic bacteria exhibit __________ hemolysis on blood agar
Beta-
No
Gamma-
Alpha-
29
Most pathogenic bacteria are:
Dolicophiles
Mesophiles
Thermophiles
Psychrophiles
30
The stain that is primarily used to detect spirochetes and rickettsiae is:
Giemsa
Carbol fuchsin
Gram stain
Ziehl-Neelsen
31
The first step in collecting samples for dermatophyte testing is:
Plucking a few skin scales
Brushing the hair with a toothbrush
Plucking some hairs
Cleaning the area to minimize surface contamination
32
The application of undiluted urine directly to the culture and sensitivity test plate is referred to as the:
Direct sensitivity test
Indirect sensitivity test
Standardized disk susceptibility method
Bauer-Kirby method
33
Routine cultures are incubated for _____ hours before initial examination.
72
18
48
30
34
Which media contain antibacterial substances thus, only allowing specific bacteria to grow?
Selective
Differentiate
35
Which media is recommended for culture and sensitivity testing?
Chocolate agar
Mueller-Hinton
MacConkey
Blood agar
36
The preferred method for the testing for dermatophytes is to:
Make a smear of a skin scraping and examine it at 40× magnification
Hold a Wood's lamp over the animal/check the skin and coat for fluoresc
Pluck hairs from periphery of the lesions and perform a culture
Make an impression smear of the moist underside of a scab and examine it
37
A type of culture media that contains additives that detect certain biochemical reactions of the bacteria is:
Fastidious
Selective
Differential
Enriched
38
Before shipping samples to an outside laboratory, samples should be
left to dry out so they won’t survive.
frozen
held for a few days before shipment
placed in a secure, leakproof container and labeled properly.
39
Bacterial samples from hollow organs are usually collected by:
Aspiration
Punch biopsy
Swabbing
Imprint
40
A useful stain for the evaluation of fungal organisms is:
Gram stain
Ziehl-Neelsen stain
Lactophenol cotton blue
Endospore stain
41
For best results, microbiology samples must be collected:
Aseptically
At necropsy
Before physical examination
After treatment has started
42
Acid-fast stains are used primarily for the identification of __________.
Anaerobes
Mycobacterium
Pseudomonas
Viruses
43
The preferred culture media for performing the sensitivity test is
EMB agar
Blood agar
Mueller-Hinton
Bauer-Kirby
44
A streptococcal colony with a clear zone of hemolysis on a blood agar plate is
beta-hemolytic.
hemolytic
gamma (non-) hemolytic
alpha-hemolytic.
45
A useful stain for the evaluation of fungal organisms is:
Lactophenol cotton blue
Ziehl-Neelsen stain
Endospore stain
Gram stain
46
An organism with growth that is inhibited in the absence of oxygen is described as:
Facultative Anaerobes
Capnophilic
Obligate Aerobe
Microaerophilic
47
MAC is selective for gram + Bacteria
true
false
48
Culture specimens from Microsporum or Trichophyton should be collected from:
Anywhere on the open surface of the lesion
The periphery of the lesion
The discharge that is draining from the lesion
The center of the lesion
49
Transport media is used to keep microbes alive but do not encourage growth
true
false