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A Monday Quizz!

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Molecular Biology Basics!

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Questions 8

Answers

1
Telomeres
Are present at the end of bacterial chromosomes
get progressively longer in somatic cells as they age
Buffer the shortening at the 3'end of DNA during replication
Are present at the ends of eukaryotic chromosomes
2
A 12,000 bp linear DNA fragment is cut with Eco R1. About how many DNA fragments will result?
48
3
4
5
3
Restriction enzymes are
Exonucleases
Naturally occurring in many bacteria
Restricted to eukaryotes
Produced by bacteriophage
4
A rice genomic clone in pBluescript in E. coli will not produce a protein because
E. coli cannot process introns
Prokaryotes use a vastly different genetic code than rice
All of the above are true
It lacks a promoter
5
Bacteria protect their own DNA from the restriction enzymes they produce by
Inactivation restriction enzymes during replication
Methylating their DNA at recognition sites
Acetylating their DNA at the recognition site
Editing the recognition site from their DNA
6
Synthesis of oligonucleotides in vitro
Is prohibitively expensive
Is accomplished using recombinant polymerase enzymes
proceeds in the 5' to 3' direction
Uses nucleotides with the reactive groups protected
7
The prominent band on this gel is
Supercoiled plasmid
denatured plasmid
Relaxed circular plasmid
digested plasmid
8
The Lambda cut with HindIII in this gel was
Contains two fragments that have annealed via cos ends
The marker on this gel is not Lambda
heated
Denatured to prevent cos ends annealing