stud
bud
Sign up
Log in
Home
Library
Explore
SAT
More
BIOL 3200 Exam 1 Flashcards | StudBud
Explore
/
BIOL 3200 Exam 1
BIOL 3200 Exam 1
45 terms
·
StudBud Administration
·
576 plays
Imported from external platform
Flashcards
45 cards
F
Practice quiz
P
Host live
L
Questions
45
Answers
1
What is shown in this image?
sperm cells
ribosomes translating mRNA
Borrelia burgdorferi
anaphase
2
You discovered a live bacteria and want to know more about its internal structures. Which is the best microscope to use?
Darkfield Microscope
Brightfield Microscope
Phase Contrast Microscope
Scanning Electron Microscope
3
Scanning electron microscopy has higher resolution than Transmission electron microscopy
True
False
4
Dark field microscopy can resolve very thin objects like flagella or spirochetes.
True
False
5
Which of the following is a function of membrane proteins.
ion transport and energy storage
all of the above
structural support
detect environmental signals
6
Which of the following is NOT an exception to Koch's Postulates?
the activity of the immune system
many microbes will not grow on media
many microbes are nonpathogenic
7
Gram negative bacteria have a more complex cell wall than Gram positive bacteria.
True
False
8
Who created the three-domain model of life that we use today?
Herbert Copeland
Ernst Haeckel
Carl Woese
Frederick Griffith
9
What is the minimum size of a known microbial cell?
0.2 millimeter
0.2 micrometer
1.0 millimeter
1.0 centimeter
10
What is the definition of parfocal?
the ability to change objective lenses and remain in focus
the distance between the center the objective lens and its focus
the ability of how well we can see with a microscope
the magnification of ocular multiplied by that of the objective lens.
11
What is an example of a differential stain?
simple stain
Gram stain
safranin
methylene blue
12
Who developed the first vaccines based on attenuated strains?
Alexander Fleming
Rosalind Franklin
Louis Pasteur
Edward Jenner
13
What is the best explanation for a Gram-negative bacterium appearing purple after performing a Gram stain?
ethanol was left on too long
safranin was left on too long
ethanol was washed away too early
crystal violet was left on too long
14
Excitation wavelength < Emission wavelength in fluorescence microscopy.
False
True
15
What is number 6 in a Gram positive cell?
tetrapeptide bridge
sugar bond
pentaglycine bridge
peptide bond
16
What is the significance of having capsule in a bacterial cell?
it protects a cell from phagocytosis
it protects a cell from osmotic stress
it helps maintain cell shape
it looks pretty
17
Who build the first compound microscope?
Antoine van Leuuwenhoek
Francesco Redi
Robert Hooke
Edward Jenner
18
Who disproved spontaneous generation?
Louis Pasteur
Lazzaro Spallanzani
Francesco Redi
19
What is number 2 in a Gram positive cell?
D-Ala
DAP
L-Lys
20
Which of the following is NOT true?
each microbe has the capacity to reproduce its own kind
viruses can be 10x smaller than microbes
a microbe is a living organism that requires a microscope to be seen
bacteria and archaea are more closely related to each other than to eukarya
21
Immersion oil decreases the resolution of an image
False
True
22
What is the antigen portion of LPS?
O-polysaccharide side chain
oligosaccharide core
Lipid A
23
Why could Sergei Winogradsky NOT use Koch's Postulates to discover lithotrophs?
Lithotrophs do not result in disease
Koch's postulate is only used to observe extremophiles
The postulates were not published
Lithotrophs are classified as archaea and not bacteria
24
Viruses are measured in nm and bacteria are measured in μm
False
True
25
Which of the following is an exception to the definition of a microbe?
All of the above
Thiomargarita namibiensis
biofilms
viruses
26
Which molecule is the base of the tetrapeptide chain in peptidoglycan?
DAP
NAG
L-Lys
NAM
27
Which of the following is true about the Gram-positive cell walls?
It contains lipopolysaccharide
It is made up of three or more layers of peptidoglycan
It contains porins
It is ONLY made up of N-acetylmuramic acid
28
Who proposed Endosymbiotic Theory?
Lynn Margulis
Lady Mary Montagu
Lazzaro Spallanzani
Rosalind Franklin
29
Archaea have ether bonds and bacteria have ester bonds.
False
True
30
Polar or charged molecules can diffuse freely through the membrane via passive transport.
True
False
31
The numerical aperture of light that enters substance at θ=66° is 1.197. What is the substance?
water
air
ice
glass
32
What was Frederick Griffith's contribution to microbiology?
he used X-ray crystallography to determine structure of DNA
he discovered transformation in bacteria
he added Fungi as the fifth kingdom of eukaryotic microbes
he developed the ultracentrifuge
33
The resolution of a compound microscope is 0.2 μm
False
True
34
If you switch from low (10X) to high (100X) magnification objective lens, the light cone __, and N.A. becomes?
narrows; higher
widens; lower
widens; higher
narrows; lower
35
What kind of microscopy was used to generate this image?
SEM
TEM
Darkfield
Flourescent
36
What is the best definition for bioremediation?
use bacteria to clone and produce insulin
exploits differences in refractive indices
altering genetic sequences
clean human wastes and pollutants
37
Which of the following statements is correct about a patient who died from endotoxin?
Antibiotic treatment led to peptidoglycan destruction
Bacillus anthracis may have contributed to the patient's death
The patient had antibiotic treatment against Gram-negative bacteria
The methicillin-resistant S. aureus contributed to the death of the patient
38
What is the counterstain for a Gram stain?
safranin
methylene blue
crystal violet
carbol fuschin
39
What did Carl Woese use to distinguish Archaea and Bacteria?
tRNA
28S mRNA
16S rRNA
16S DNA
40
What is the largest magnification possible for a microscope that you use in lab?
400x
100x
1000x
10x
41
What is the definition of refraction?
the bending of light as it enters a substance that increases its speed
the bending of light as it enters a substance that decreases its speed
42
The first vaccination was performed by ____, and the first antibiotic was discovered by _____.
Louis Pasteur; Edward Jenner
Edward Jenner; Alexander Fleming
Louis Pasteur; Alexander Fleming
Lady Montagu; Louis Pasteur
43
The resolution of the human retina is about 150 μm.
False
True
44
What is the definition of numerical aperature?
the ability of a substance to bend light
the ability of how well we can see with a microscope
the smallest distance by you can tell apart two objects
the ability of a lens to gather light
45
Which of the following is evidence for the Endosymbiotic Theory?
Chloroplasts have circular DNA
mitochondria possess 16s rDNA and rRNA
both Red and Yellow
the nucleus is surrounded by a nuclear membrane
