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BACE Practice Exam..
BACE Practice Exam..
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Practice for BACE exam
Flashcards
100 cards
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Practice quiz
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Questions
100
Answers
1
A structural gene’s code is 5' TAC ATG CCC TTA ATC 3', what will the mRNA transcript be?
3' AUG UAC CCC AAU UAG 5'
3' ATG TAC GGG AAT TAG 5'
3' AUG UAC GGG AAU UAG 5'
3' AUG ATC GGG AAT UAG 5'
2
Match the compound to its corresponding bond type. CH4
Nonpolar Covalent
Ionic
Polar Covalent
3
Which of the following is true regarding the handling of biohazardous waste?
It does not need special labeling.
It does not require special handling.
must be segregated from other chemical and laboratory waste
4
Solve and round to the correct sig figs 12.00 / 6.0
2
2.00
0.50
2.0
5
Cytoplasmic, circular DNA containing only a few genes
Prokaryotic DNA
Eukaryotic DNA
Plasmid DNA
6
This lab safety signs mean :
Radiation
Biohazard
Toxic
Explosive
7
Participates in the production of commercial quantities of a product
Research and Development
Quality Control (QC)
Quality Assurance (QA)
Manufacturing
8
A solution with a pH of 3.0 is how many times more acidic than a solution with a pH of 6.0?
10,000
1000
10
100
9
Staining with Coomassie Blue
To coat the sample proteins with a negative charge/denature
To determine the relative size of the samples run on the gel
To facilitate loading samples into wells
To visualize proteins after PAGE
10
A restriction enzyme digestion reaction contains which of the following reagents?
DNA, Water, Restriction Enzyme
DNA, Water, Buffer, Restriction Enzyme
DNA, Water, Buffer, dNTPs
DNA, Water, dNTPs, Restriction Enzyme
11
Match the compound to its corresponding bond type. H2O
Polar Covalent
Ionic
Nonpolar Covalent
12
What is the best definition of the term “hypothesis” as it is used in science?
A assumption based ona verifiable law and can be proven true
A testable explanation for a scientific problem or question.
A scientific model.
A comprehensive set of ideas explaining a natural phenomenon
13
Which structure regulates the flow of molecules into and out of the cell?
Plasma membrane
Mitochondria
Nucleus
Endoplasmic reticulum
14
What is the purpose of using the quadrant method when streaking bacteria on agar plates?
To allow more bacteria to be spread across the plate
To provide four equal parts for the bacteria to grow
To dilute individal bacterial cels bcoming isolated colonies
All of the above
15
Example of polar covalent bond.
O2
H2
H2O
16
When using a microscope on the highest power objective, which focus knob should you use?
Coarse
Fine
17
In the U.S., cGMPs are regulated by which agency?
The U.S. Department of Agriculture (USDA)
The Environmental Protection Agency (EPA)
The Occupational Safety and Health Administration (OSHA)
The Food and Drug Administration (FDA)
18
How is the number 0.000052 correctly written in scientific notation?
5.2 x 10 exponent is 5
None of the above
0.000052
5.2 x 10 exponent is -5
19
Gram Staining Procedure - Counterstain
Safranin Stain
Crystal Violet
Gram's Iodine
20
The eyepiece of a microscope gives how many times magnification?
40x
10x
400x
20x
21
What parts of the microscope are parts D and B
Occular lens and focusing knob
Base and Stage
Stage and Base
Objective lens and focusing knob
22
Which of the following agencies is responsible for regulations pertaining to worker safety?
The Food and Drug Administration (FDA)
The Occupational Safety and Health Administration (OSHA)
The U.S. Department of Agriculture (USDA)
The Environmental Protection Agency (EPA)
23
Place the following step in the best description of the sequence of a PCR cycle. " 2 "
The primers hybridize to the target DNA
DNA polymerase extends the primers to make a copy of the DNA
The mixture is heated to denature the double stranded DNA.
24
Which microscope parts are used when carrying a microscope?
light source
stage
base
arm
25
Parts of Microscope
Fine Focus
Coarse focus
Base
Zoom
26
Circular, long DNA containing several thousand genes
Prokaryotic DNA
Eukaryotic DNA
Plasmid DNA
27
Which of the following serves as the decolorizer in a Gram stain?
Crystal violet
Alcohol
Gram safranin
Iodine
28
Correct Scientific notion for 1,230,000
1.23x106
123x104
1.23x10-6
12 x 105
29
Match the macromolecule to its example and associated functions.
Nucleic Acid: Cellulose; Energy Storage/Receptors
Nucleic Acid: Cholesterol; Membrane Structure/Energy Storage
Nucleic Acid: RNA; Information Storage and Transfer
Nucleic Acid: Enzymes; Structure/Receptors/Transport
30
Which features of bacterial plasmids can be used as a marker when growing bacterial cultures?
Antibiotic resistance gene
Origin of replication
Arabinose operon
Multiple cloning site
31
Gram positive, or gram negative?
Gram +ve
Gram -ve
32
Gram negative or gram positive?
gram negative (pink)
gram positive (purple)
33
In recombinant DNA technology, DNA ligase is used to rejoin which of the following bonds in DNA
Phosphodiester
Hydrogen
Nitrogen
Carbon
34
How does Coomassie Brilliant blue work in the Bradford assay?
Dye binds to protein and turns brown
Dye oxidizes protein and turns brown
Dye binds to protein and turns blue
Dye oxidizes proetin and turns blue
35
Why is plasmid DNA is purified using anion exchange column chromatography?
DNA has a natural affinity for most columns
Plasmid DNA is small enough to bind to the column
DNA is a positive charge, binds to the negative charge resin
DNA is a negative charge, binds to the positive charge resin
36
Which is a controlled document that contains instructions on how to perform a routine activity
Document Log
Batch Record
Test Record
Standard Operating Procedure
37
What is this called?
Plate reader
Centrifuge
Thermocycler
Spectrometer
38
Calculate the following and round to the correct sig figs: 0.93 + 1.8
2.73
3
2.7
2.730
39
If the total magnification of a light microscope was 500 x
Objective lens magnification = 490 x
Objective lens magnification = 50 x
Eyepiece lens magnification = 490 x
Eyepiece lens magnification = 50 x
40
Match the macromolecule to its example and associated functions.
Carbohydrates: Cellulose; Energy Storage/Receptors
Carbohydrate: Cholesterol; Membrane Structure/Energy Storage
Carbohydrates: Enzymes; Structure/Receptors/Transport
Carbohydrates: RNA; Information Storage and Transfer
41
Cocci are bacteria that are:
Round-Shaped
Flat-Shaped
Spore-Shaped
Rod-Shaped
42
In agarose gel electrophoresis, the negative electrode terminal is located:
At the top of the gel (closest to wells)
At the bottom of the gel (furthest from the wells)
43
When microorganisms (usually yeast) undergo fermentation, they obtain their energy by:
Aerobic Respiration
Anaerobic Respiration
Photosynthesis
Chemosynthesis
44
What is an example of an covalent bond?
AlF3
NaCl
H2O
CaO
45
Place the following step in the best description of the sequence of a PCR cycle. " 1 "
The mixture is heated to denature the double stranded DNA.
The primers hybridize to the target DNA
DNA polymerase extends the primers to make a copy of the DNA
46
Which of the following techniques should be used to amplify specific DNA sequences?
PCR
PAGE
ELISA
Bradford assay
47
How does DNA differ from one species to another?
The number of genes and non-coding regions
The directionality of the DNA strands
The type of nitrogenous bases the DNA contains
The way the nitrogenous bases pair together
48
When exposed to a foreign antigen, the normal human immune system response is to produce
Red blood cells
Hormones
Hemoglobin
Antibodies
49
Gram Staining Procedure - Dye
Gram's Iodine
Safranin Stain
Crystal Violet
50
Match the macromolecule to its example and associated functions.
Lipids: Enzymes; Structure/Receptors/Transport
Lipids: Cholesterol; Membrane Structure/Energy Storage
Lipids: RNA; Information Storage and Transfer
Lipids: Cellulose; Energy Storage/Receptors
51
Which of the following techniques destroys all microorganisms?
Sanitization
Cleansing
Sterilization
Disinfection
52
The observable traits of an organism are referred to as the:
Phenotype
Karyotype
Genotype
Merotype
53
GLP is a quality system intended to regulate compliance in preclinical laboratory studies
False
True
54
Scientific Notation: 0.05
5 x 102
.5 x 10-2
0.05 x 102
5 x 10-2
55
Your run a PCR reaction with products in size from 200-400 bp. Which strategie will give best results of the products?
high precent of agrose and high running voltage
high precent of agrose and low running voltage
low precent of agrose and high running voltage
low precent of agrose and low running voltage
56
Place the following step in the best description of the sequence of a PCR cycle. " 3 "
The mixture is heated to denature the double stranded DNA
The primers hybridize to the target DNA
DNA polymerase extends the primers to make a copy of the DNA
57
When labeling Petri plates, what information should be included on the plate?
The date/medium used
The name of the microorganism, or the sample being plated
All of the above
The initials/name of the technician performing the plating
58
Which of the following is the primary feature of chemically competent cells
They are less susceptible to infection.
They are more permeable to proteins.
They are less susceptible to lysis.
They are more permeable to DNA.
59
Why are biopharmaceuticals typically more difficult to manufacture
Biopharmaceuticals cannot be heat sterilized
Biopharmaceuticals typicaly require special handling/storage
All Of The Above
Biopharmaceuticals are more prone to contamination.
60
What parts of the microscope are parts D and B
Stage and Base
Objective lens and focusing knob
Occular lens and focusing knob
Base and Stage
61
Bacilli are bacteria that is rod-shape
True
False
62
Adding loading dye to protein samples
To facilitate loading samples into wells
To visualize proteins after PAGE
To determine the relative size of the samples run on the gel
To coat the sample proteins with a negative charge/denature
63
Which is a controlled document that contains instructions on how to manufacture a product
Test Record
Document Log
Batch Record
Standard Operating Procedure
64
which of the following is the best next step after streaking the plates with bacteria?
Place plates rightside up then confirm temperature is corect
Confirm temperature is correct then place plates upside-down
Confirm temperature is corect then place plates rightside up
Place plates upside-down then confirm temperature is correct
65
What does this lab safety sign mean?
Flammable Material
Wash Hands
Environmental Hazard
Corrosive Material
66
Solve and round to the correct sig figs. 72.5654 + 5.32
77.9
77.89
77.8854
77.88
67
The U.S. agency responsible for regulating biopharmaceuticals for use in humans is:
The U.S. Department of Pharmaceuticals (USDP)
The Environmental Protection Agency (EPA)
The Food and Drug Administration (FDA)
The U.S. Department of Agriculture (USDA)
68
What is 0.9857 rounded to three significant digits?
0.986
0.990
0.985
0.100
69
Example of ionic bond
NaCl
Fe
CO2
Cl2
70
DNA is __________ into RNA, which is ___________ into protein.
translated, transcribed
transcribed, transfected
transfected, translated
transcribed, translated
71
Match the macromolecule to its example and associated functions.
Proteins: RNA; Information Storage and Transfer
Proteins: Enzymes; Structure/Receptors/Transport
Proteins: Cholesterol; Membrane Structure/Energy Storage
Proteins: Cellulose; Energy Storage/Receptors
72
Bradford assay turns blue when bound to proteins. What does color intensity in the Bradford Assay signify?
More blue = more Bradford Assay
More blue = more proteins
More blue = more water
More blue = more plant matter
73
Works to identify new or improved products or processes
Research and Development
Quality Control (QC)
Quality Assurance (QA)
Manufacturing
74
Which is typically measured using a spectrophometer set at a wavelength of 600 nm?
The concentration of DNA in a sample
The concentration of bacteria in a suspension
The number of bacterial colonies on a plate of media
The number of DNA base pairs in a sample
75
Cellulose important raw material for textiles. Which of the following is a function of polysaccharides in the cell
Recognition of receptor proteins on other cells
none of the above
Transporting molecules throughout the cell
Providing structure and energy storage within cells
76
Adding SDS to the sample buffer and the PAGE running buffer
To facilitate loading samples into wells
To coat the sample proteins with a negative charge/denature
To determine the relative size of the samples run on the gel
To visualize proteins after PAGE
77
What is a disadvantage to using prokaryotes in the production of biopharmaceuticals?
Prokaryotes cannot fold and assemble more complicated eukaryotic proteins
none of these
Prokaryotes cannot accept mammalian DNA
Prokaryotic cultures are more easily contaminated than eukaryotic cultures.
78
A single base pair change in a gene’s DNA sequence can affect shape/function of the protein.
True
False
79
Which department is responsible for document control/regulatory compliance
Quality Control (QC)
Quality Assurance (QA)
Marketing
Biomanufacturing
80
SDS-PAGE Steps
Stain gel and analysis, Prep Protein, Load samples, electrophoresis,
Load samples, electrophoresis, Prep Protein Stain gel & analyze
Electrophoresis, Stain gel & analyze, Prep Protein, Load samples,
Prep Protein, Load samples, electrophoresis, Stain gel & analyze
81
Why must technicians practice aseptic technique when culturing cells?
To ensure they incubate their cells at proper temperature
To ensure they're growing pure cultures of the desired cells
To ensure they're usin the right media to grow desired cells
To ensure they add the correct supplements to their media
82
4.51 * 2.3 (round to the correct sig figs)
10.4
10.3
10.
10
83
Ensure that all government regulations are followed, working closely with all departments
Research and Development
Quality Control (QC)
Manufacturing
Quality Assurance (QA)
84
Select the option that represents the correct order of assembly in the cell:
Polypeptide, Amino Acid, Protein
Protein, Amino Acid Polypeptide
Amino Acid, Protein, Polypeptide
Amino Acid, Polypeptide, Protein
85
“Bioethics” is best described as:
The study of biology as it relates to changes in society
moral decisions tht must be made bc advances in biology/tech
The evolution of better adapted organisms
The part of the brain responsible for making moral decisions
86
Which is an example of a non-polar covalent bond?
CN
H2O
F2
HCl
87
Gram Staining Procedure - Decolorizer
95% Ethyl alcohol/acetone
Safranin Stain
1:10 Bleach/Water Solution
88
Which of the following is the study of the full complement of DNA in a cell?
Metabolomics
Genomics
Transcriptomics
Proteomics
89
How are monoclonal antibodies made?
Antigens are integrated with antibodies
B lymphocytes and tumour cells fused
Antibodies are cloned
Antigens attach to T cells
90
Which type of ELISA determine the concentration of a specific antigen in a sample
Qualitative ELISA
Either will work
Quantitative ELISA
91
Linear, long pieces of DNA with many thousands of genes, contained within a nucleus
Eukaryotic DNA
Plasmid DNA
Prokaryotic DNA
92
Match the compound to its corresponding bond type. NaCI
Nonpolar Covalent
Polar Covalent
Ionic
93
The three-dimensional structure of a protein is largely determined by the amino acid sequence.
False
True
94
Why is
E. coli
usually incubated at 37°C?
LB agar is most stable at 37°C
The maximum growth rate for *E. coli* occurs at 37°C.
E. coli only grows at 37°C.
95
Conducts testing on raw materials, in-process samples, and finished products.
Manufacturing
Research and Development
Quality Control (QC)
Quality Assurance (QA)
96
Adding a molecular weight standard to an empty well
To visualize proteins after PAGE
To facilitate loading samples into wells
To determine the relative size of the samples run on the gel
To coat the sample proteins with a negative charge/denature
97
Which of the following is the study of the entire set of proteins of a cell or organism?
Metabolomics
Proteomics
Transcriptomics
Genomics
98
True/False: In gel electrophoresis for smaller DNA fragments you have to use more agrose/higher voltage
false
true
99
Spirilla
Disease transmitted through touching
Bacteria that cause HPV and HBV
Spiral or corkscrew-shaped bacteria that cause syphilis
Cause only Lyme disease
100
A Bradford assay measures...
concentration of protein in a sample
purity of Cin8 elution
nucleic acid content
concentration of Cin8
