Clin Path Lecture 9

Percentage depends on the number of mature RBCs present as well as the number of reticulocytes present so it can

Splenic sequestration: splenomegaly, anesthesia, heparin treatment in horses

About 3-4 days are required for increased synthesis and release of reticulocytes from bone marrow

Duration of anemia is about ___ for strong reticulocyte response

Lack of blood reticulocyte response to anemia

RETIC-HGB - reticulocyte hemoglobin; average amount of hemoglobin in a single reticulocyte (depends on size + Hb conc.)

Increased polychromasia in stained blood film; absolute reticulocytosis, bone marrow evaluation (esp. horses)

Comparable to the CHr measured in the Advia 120 and Advia 2120; MCH (CH) is average amount of Hb in a single erythrocyte

A week or more is required for maturation (total disappearance of ribosomes) of punctate reticulocytes in blood

Differential diagnosis of Anemia:

Reticulocyte indices (retic MCV)/retic Hb content can help diagnose iron limited erythropoiesis _ indices are abnormal

___ reticulocytes in the blood of cats mature to punctate reticulocytes in a day or less

Physical findings of Anemia:

MCV and RDW may be increased with intense erythroid regeneration in horses

Methemoglobin is always obvious in venous blood samples; marked lipemia; agglutination

Slight, rapid ^ in blood reticulocytes (stress retics) may occur in response to severe anemia due to premature release

Absorption of colostrum; short fetal erythrocyte life spans; increased cardiovascular volume growth

Anemic animals with __ (increased numbers of reticulocytes/uL blood) are classified as having a regenerative anemia

Cause of the anemia:

Hereditary stomatocytosis in dogs; increassed osmotic fragility in Abyssinian and Somali cats

A relative (raw) reticulocyte count is the percentage of all erythrocytes that reticulocytes

What stain is being used to view these reticulocytes?

What type of anemia is this?

What type of anemia is this?

Anemias without a reticulocyte response are called ____ anemias

___ reticulocytes in dogs circulate for about a day before maturing into erythrocytes

Routine erythrocyte indices (MCV, MCH, MCHC) change ___ in association with iron deficiency

Ruminants and horses do not normally have reticulocytes in blood

Expansion of vascular space: pregnancy (not horse), postnatal growth (anemia of the neonate); overhydration

Degree of anemia:

True iron deficiency; iron-limited erythropoiesis (inflammation, portosystemic shunt); breed associated microcytosis

Classification of anemia of regenerative or nonregenerative:

Usually less than __ reticulocytes in healthy, nonanemic dogs; 0-0.5% aggregate + 1-10% punctate in healthy cats

What is indicated by the Hematocrit tube?

What is this indicative of?

Anemia is a diagnosis

The presence of __ indicates that the anemia was caused by either hemorrhage or increased erythrocyte destruction

Counting the numbers of reticulocytes in blood is critical for assessing anemia in domestic animals, except the horse

History for anemia:

Punctate as well as aggregate reticulocytes

What is indicated by the Hematocrit tube?

Low blood erythropoietin: cord blood transfusion, increased arterial pO2 and 2,3-DGP; poss. iron deficiency, esp. pigs

What stain is being used to view these reticulocytes?

What is indicated by the Hematocrit tube?

Reticulocytes may be counted in horse __ aspirates

Absolute reticulocyte counts may also be determined directly by flow cytometry in some electronic cell counters

Classification of anemia via pathophysiologic mechanisms:

Lack of ___ after 3-4 days of anemia indicated a disorder with decreased erythrocyte production

Hemolytic anemia with marked reticulocytosis; swollen erythrocytes

Sluggish response to hemorrhagic anemia

Classification of anemia with Erthrocyte indices: