The function of DNA ligase in the generation of recombinant DNA is to

join DNA fragments by the formation of phosphodiester bonds

Which is NOT a step in the process of creating a transgenic mouse with a nonfunctional gene for a protein of interest?

addition of an NLS to the gene sequence

Which of the following is NOT an application of recombinant DNA technology?

reducing the salt in environmental soil so that plants can grow

Complementary base pairing is important for

synthesizing cDNA molecules from mRNA templates

In gel electrophoresis of DNA fragments,

the fragments are separated on the basis of their sizes

Which of the following is required for a transgene to be expressed in a eukaryotic host?

transcription factor binding sites, enhancers, and a poly A-recognition seq

A DNA molecule complementary to an mRNA molecule

are the result of staggered cuts of DNA by restriction enzymes

cleave double-stranded DNA at specific palindromic sequences

forms hybrids with mRNA to prevent translation

Expression vectors are different from other vectors because they contain

regulatory regions that permit the cloned DNA to produce a gene product

can be used to make large amounts of protein in E. coli cells

Biotechnology may perhaps best be described as

collection of approaches to the exploitation of living systems- useful prod

is useful in introducing foreign DNA into plants

is used to identify and isolate DNA fragments

Which of the following statements about bacterial resistance genes is FALSE?

they are usually present in the bacterial large circular genome

DNA chip technologies can be used to

show transcriptional patterns in organism during diff times of development

a small, circular double-stranded DNA molecule that replicate autonomously

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BIO Chapter 13

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Questions 33

Answers

1
Restriction enzymes
do not cut the host bacterium's DNA
cleave DNA at sequence-specific sites
all of the above
are essential tools in molecular biology
2
The function of DNA ligase in the generation of recombinant DNA is to
replicate DNA
cut DNA
join DNA fragments noncovalently
join DNA fragments by the formation of phosphodiester bonds
3
Which is NOT a step in the process of creating a transgenic mouse with a nonfunctional gene for a protein of interest?
homologous recombination
inactivation of the gene by insertion of a reporter gene
addition of an NLS to the gene sequence
incorporation of an inactivated copy of the gene into a vector
4
Which of the following statements about typical useful recombinant gene vectors is FALSE?
they have several restriction sites
the contain reporter genes
they can reproduce independently
they are about 60,000 bp long
5
For a Ti plasmid to be propagated in a host plan cell, the vector needs
telomeres
an origin of replication
a, b, and c
centromeres
6
Which of the following is NOT an application of recombinant DNA technology?
reducing the salt in environmental soil so that plants can grow
creating bacteria that can accelerate the breakdown of wood chips and paper
creating vaccines for pathogens
making plants more resistant to insect larva
7
Gene expression can be inhibited by
a, b, and d
microRNA
knockout genes
antisense RNA
8
In recombinant DNA technology, __ may be used as a selectable marker or reporter gene
all of the above
tefr
lacZ
ampr
9
A potential disadvantage of recombinant DNA technology is that it
could make weeds herbicide-resistant
has the capacity to spread transgenes from crops to other species
all of the above
could create transgenic plants that kill beneficial insects
10
Recombinant DNA tech. mainly produces _ that r normally present in low amounts & are difficult to _; _ vectors are used
proteins; purify; expression
proteins; detect; plasmid
proteins; purify; plasmid
hormones; detect; plasmid
11
Complementary base pairing is important for
ligation reactions with blunt-end DNA molecules
synthesizing cDNA molecules from mRNA templates
hybridization between DNA and transcription factors
restriction endonucleases for cutting cell walls
12
__ is the production of pharmaceuticals in farm animals or plants
fishing
gene expression
manufacturing
Pharming
13
In gel electrophoresis of DNA fragments,
the fragments migrate towards the cathode (negative charge)
the fragments are separated on the basis of their sizes
large fragments migrate more quikly than small fragments
the fragments are separated based on their charge differences
14
RNAi
is more effective than anitsense RNA in inhibiting translation
is produced only by viruses
both a and d
requires other proteins to modify the RNAi
15
Vectors include all of the following except
bacteriophage
plant plasmids
bacterial plasmids
viruses
16
Which of the following is required for a transgene to be expressed in a eukaryotic host?
an inducible promoter and repressor proteins
a transcriptional termination site and a ribosome binding site
a DNA origin and an antibiotic resistance marker
transcription factor binding sites, enhancers, and a poly A-recognition seq
17
A cDNA clone is
mostly cytosine
a copy of the DNA identical to the nuclear gene
A DNA molecule complementary to an mRNA molecule
a copy of noncoding DNA
18
"sticky ends"
are produced by the action of all restriction enzymes
are the result of staggered cuts of DNA by restriction enzymes
have non-specific base sequences
form associations with complementary DNA that are very stable
19
Restriction enzymes
cut single-straded DNA
cut double-stranded DNA at any palindromic sequence
cleave double-stranded DNA at specific palindromic sequences
have been isolated from just a few species of microorganisms
20
Complementary RNA
forms hybrids with mRNA to prevent translation
blocks DNA replication
is sense RNA
inhibits transcription
21
For a prokaryotic vector to be propagated in a host bacterial cell, the vector needs
telomeres
centromeres
reporter genes
an origin of replication
22
Expression vectors are different from other vectors because they contain
telomeres
regulatory regions that permit the cloned DNA to produce a gene product
drug-resistance markers
DNA origins
23
Reporter genes include genes for
drug resistence
bioluminescence
both a and b
DNA origins
24
Expression vectors
are useful for analyzing RNA transcription patterns
can be used to make large amounts of protein in E. coli cells
are useful for isolating large amounts of DNA
are used to create SNPs for DNA microarrays
25
Biotechnology may perhaps best be described as
a branch of the science of molecular biology
collection of approaches to the exploitation of living systems- useful prod
an industry to make products useful to medicine
its own specific discipline
26
The Ti plasmid
is useful in introducing foreign DNA into plants
is of viral origin
replicates the host cells
is useful in introducing foreign DNA into yeasts
27
Gel electrophoresis
is used in allele-specific oligonucleotide hybridization
is required for PCR reactions
is used to identify and isolate DNA fragments
causes DNA to be pulled through gel toward the negative end of the field
28
Cloning a gene may involve
restriction endonucleases and ligase
tranformation or transfection
plasmids and bacteriophage
all of the above
29
Which of the following statements about bacterial resistance genes is FALSE?
they can confer antibiotic resistance to other prokaryotes
they are convenient selectable markers
they are important to medicine
they are usually present in the bacterial large circular genome
30
A host cell or organism that contains recombinant DNA is referred to as a __ cell or organism
selectable
transgenic
chimeric
transfected
31
DNA chip technologies can be used to
make transgenic plants
show transcriptional patterns in organism during diff times of development
predict an individual's likelihood of getting cancer
clone DNA
32
A plasmid
a small, circular double-stranded DNA molecule that replicate autonomously
is the bacterial genome
does not code for proteins
is only recombinant
33
The RNA in RNAi (RNA interference) is
single stranded
relatively stable in cells
capable of binding to specific mRNAs
both c and d

BIO Chapter 13 Flashcards | StudBud