Just as One-Eyed Mike (Ziv Zaifman) arrives in the Michigan metropolis of Great Lakes City, he reunites with his old friends from his first adventure, The R and S Gang and falls in love with Sid (Leah Mei Gold) as he battles his nefarious mad scientist foe (voiced by Nick Kroll) for the future of the animal kingdom with the help of a conqueror (voiced by Steve Buscemi) and three sharks (voiced by Barry Humphries, Eric Bana and Bruce Spence).