Chapter 3 Exam 1 Review

Question 1

Isoelectric focusing is used to determine what about a protein

Accepted Answer

pH

Question 2

A conjugated protein has permanently chemical components in addition to amino acids

Accepted Answer

True

Question 3

Aromatic amino acids are capable of absorbing UV light, what is the wavelength range?

Accepted Answer

270-280 nm

Question 4

Only L configuration amino acids are used to make proteins in biological systems

Accepted Answer

True

Question 5

Peptides and proteins are formed when amino acids are joined together in CONDENSATION REACTIONS to form ______

Accepted Answer

peptide bonds

Question 6

Specific activity is the total amount of protein in a sample

Accepted Answer

False

Question 7

How can other amino acids other than the 20 covered in class be present in proteins?

Accepted Answer

Post translational modifications

Question 8

Which of the following is not a type of Protein?

Accepted Answer

Mitochondria

Question 9

Tandem Mass Spectrometry can help determine amino acid sequencing. Why is it useful?

Accepted Answer

Shows differences in protein sequences that indicate divergences

Question 10

Proline is NOT the only amino acid that does not have a +NH3 alpha carbon amino group

Accepted Answer

False

Question 11

If an animo acid has a non-amino acid part of a conjugated protein, it is called a

Accepted Answer

prosthetic group

Question 12

The isoelectric point (pl) is where the + and - charges of a Zwitterion are balanced and the net charge is ZERO

Accepted Answer

True

Question 13

What does SDS (an anionic detergent) do to a gel electrophoresis page

Accepted Answer

Denatures everything (everything -)

Question 14

At acidic pH, what happens to functional groups

Accepted Answer

They become protonated (+)

Question 15

To calculate the pl of Histidine, you have to subtract the pH of the +1 and -1 pH levels

Accepted Answer

False

Question 16

What of the following amino acids are Non-polar, aliphatic?

Accepted Answer

Methionine (M)

Question 17

Which of the following can column chromatography NOT be separated by

Accepted Answer

pH

Question 18

What amino acid is NOT chiral?

Accepted Answer

Glycine (G)

Question 19

What is the "weighted average" of an amino acid

Accepted Answer

110

Question 20

Gel electrophoresis separates based on affinity

Accepted Answer

False

Question 21

Cysteine is capable of disulfide bonds

Accepted Answer

True

Question 22

What pH range is Histidine primarily in the +2 charge state?

Accepted Answer

<1

Questions 22