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cell fractionation and ultracentrifugation Flashcards | StudBud

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cell fractionation and ultracentrifugation

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A biology quiz to make sure lower sixth students understand the basics of cell fractionation.

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Questions 8

Answers

1
what is the second step of cell fractionation?
centrifugation
homogenization
cutting of tissues
filtration of the suspension from the homogenate
2
why is a cold solution required
so the pH stays the same
to reduce osmosis
to reduce enzyme activity
so you don't burn yourself
3
whose presentation was the best so far?
didn't pay attention to any of them, they were all boring
what kind of a question is this?
does it matter?
Melis', duh!
4
what does the buffer solution do?
prevent organelles from shrinking
prevent organelles from denaturing
prevent organelles from bursting
stabilize the pH
5
what is the fluid on top on the precipitate called?
supernatant
filtrate
homogenate
isotonic
6
what is the independent variable of differential centrifugation?
the temperature at which the organelles are spun
the amount of time organelles are spun for
the speed at which the organelles are spun
the type of cell used
7
why is an isotonic solution required
to separate the organelles properly
so the pH stays the same
so the water potential stays the same
to the organelles from being broken down
8
what is the purpose of cell fractionation?
because biologists have nothing better to do
to break down tissues
to break down cells
to study specific organelles