Molecular Biology Basics

Question 1

True or false: DNA strands run parallel

Accepted Answer

False

Question 2

How is RNA synthesized during transcription?

Accepted Answer

5' to 3'

Question 3

What is the percentage of bases in complementary RNA?

Accepted Answer

5% U, 10% C, 12% G, 15% A

Question 4

What RNA processing steps are needed before export?

Accepted Answer

3' poly A tail added, 5' cap added, Introns removed, exons joined, All of these

Question 5

Which are products of transcription?

Accepted Answer

mRNA, rRNA, tRNA, snRNA, small RNAs

Question 6

What is different in prokaryotic vs eukaryotic transcription?

Accepted Answer

Both a and b, Primary RNA transcript is final mRNA in prokaryotes, Transcription and translation coupled in prokaryotes

Question 7

True or false: RNA has thymine

Accepted Answer

False

Question 8

What associates with RNA polymerase during RNA synthesis?

Accepted Answer

Nucleoside triphosphate

Question 9

Which molecules are nucleic acids?

Accepted Answer

RNA, DNA

Question 10

What is the RNA transcript for 3'-AGCTATA-5'?

Accepted Answer

3'-UCGAUAU-5'

Question 11

What are the roles of 5' cap and 3' poly A tail?

Accepted Answer

All of these, Protects RNA, Provides stability, Helps during translation

Question 12

Why are promoters AT-rich?

Accepted Answer

A-T bonds are weaker

Question 13

True or false: RNA transcript is same as template DNA

Accepted Answer

False

Question 14

What does RNA polymerase do in prokaryotes?

Accepted Answer

Elongates transcript, Forms RNA-DNA duplex, Separates DNA, All of these

Question 15

What is the complementary DNA strand for 5'-AGCTATA-3'?

Accepted Answer

3'-TCGATAT-5'

Question 16

What are the nucleotide percentages in bacterial DNA?

Accepted Answer

10% T, 40% G, 40% C

Question 17

What is the central dogma of molecular biology?

Accepted Answer

Both transcription and translation

Question 18

What is needed for eukaryotic transcription initiation?

Accepted Answer

DNA template with promoter, All of these, RNA polymerase, General transcription factors

Question 19

How do RNA nucleotides differ from DNA?

Accepted Answer

Both a and b, Uracil instead of thymine, Ribose instead of deoxyribose

Questions 19