Micro Lab Exam 1

The following chemical is used as decolorizer in Gram staining

Which reagent is added to the wells during the second step of Indirect ELISA test

A false-negative Gram reaction might occur if the bacteria is too old

What was used as a mordant for acid-fast staining?

In Gram staining decolorizer will

To focus light on the specimen

Which of the following lens has the smallest field of view

The correct order for Gram staining is Primary stain, Decolorizer, Mordant, Counter stain

At the following stage you for the first time observe a difference between Gram +ve & Gram -Ve

What is the Gram reaction of the bacterium shown here?

You should always begin viewing a slide under low power or scanning because…

Wearing closed shoes is a part of aseptic technique.

Bacillus and Clostridium are medically important endospores forming bacteria

To obtain better results it is important to take culture several times

The ability to distinguish between objects is called

In the Lab experiment, Serratia marcesens was resistant to the effects of UV radiation

Pour plate technique is not a quick and easy technique to obtain pure bacterial culture

The bacteria showing here is stained with

UV light is mainly used to disinfect air and surfaces

Scanning lens has the shortest working distance

Light repair of UV damage consists of both endonuclease and pyrimidine dimerase activity

Which of the following samples would you not perform an acid-fast stain on?

Coarse focus is used to focus light on the specimen

Lysozyme kills Gram positive bacteria

What cell wall material is reacts in acid-fast reaction

In acid-fast negative staining result the bacteria will stain

Oil immersion lens has the longest working distance

What gram reaction would you expect if you used the endospore decolorizer by accident?

Coarse focus is used to focus when using the oil immersion lens

The results shown here (arrow) are the results of

You may get a false-negative endospore staining result if

The media used to test for antibiotic sensitivity is called

To perform a direct simple stain one would use a

Which technique is used for counting the # of colony-forming bacteria present in liquid culture

What is the Gram reaction of the bacterium shown here?

Which of the following methods of control is/are bactericidal?

What would be the last reagent added to Indirect Elisa test

which of the following is not used in the Gram staining

Salt tolerant bacteria would be expected to be found

The antibiotic penicillin is most effective against

Long time exposure at a long distance from the bacteria will have highest killing

500X total magnification is achieved with the

Fine focus is used to

If you do not quickly pass the smear through heat

Albumin is used in the lysozyme activity as a negative control

Long time exposure at a short distance from the bacteria will have greatest killing

The decolorizer for the Acid-fast staining is

Parfocal is

Mueller-Hinton agar is used for

Heat inhibits the growth of bacteria by damaging the DNA

What is the name of this test?

With ocular lense of 20x the total magnification of 80X can be achieved with the

What staining procedure was performed on this slide?

A false-positive Gram reaction might occur if

The distance between the objective and the slide is called working distance

It is not important to flame the loop between streaks

Why was heat used during the endospore staining procedure?

Which of the following is a quick way to obtain pure bacterial culture

What gram reaction would you expect if you used the acid-fast decolorizer by accident?

The wire loop should be flamed and immediately used to pick up bacteria.

The lid of Petri dish should be completely removed and immediately closed after inoculation

Peptidoglycan in the cell wall gives purple color in the Gram staining

In acid-fast staining procedure on bacteria that were +ve for endospores the endospore will

Which of the following bacteria can tolerate high salt concentrations

The amount of light on the specimen is controlled by

Which bacterium was able to tolerate a high salt concentration?

In Kirby-Bauer antibiotic testing, the zone sizes are looked up on a chart to give a result of

Which of the following technique is used to determine the cell wall composition

UV kills bacteria cells by denaturing enzymes

Flaming the loop after taking bacteria will not help prevent contamination of bacterial culture

Salt and sugar inhibit growth by drawing water of the bacteria

It is important to write your name when labeling a plate